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Probiotic composition beneficial to improvement of immunity and application thereof

A probiotics and immunity technology, applied in the field of microorganisms, can solve the problems of not evaluating the comparison of the effect of the composition and the single bacteria alone, not evaluating the effect of probiotics on the permeability of the intestinal mucosa, etc., so as to strengthen the intestinal mucosa. Barrier, improve NK cell killing rate, good synergistic effect

Active Publication Date: 2020-12-04
THANKCOME BIOLOGICAL SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these patents mainly use the allergic model to evaluate the effect of probiotic intervention, and use the immunosuppressive model to evaluate the effect of probiotic intervention, and hardly evaluate the effect of probiotics on the permeability of the intestinal mucosa
Moreover, when these patents formed the strain composition, they did not evaluate the comparison of the composition and the effect of a single bacterium alone

Method used

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  • Probiotic composition beneficial to improvement of immunity and application thereof
  • Probiotic composition beneficial to improvement of immunity and application thereof
  • Probiotic composition beneficial to improvement of immunity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Isolation and identification of Lactobacillus rhamnosus NJ551 and preparation of freeze-dried powder

[0054] 1. Test material:

[0055] Sourced from 3 parts of natural fermented cheese from Inner Mongolia and Laotan fermented liquid of naturally fermented kimchi from Sichuan farmers

[0056] 2. Separation method:

[0057] 2.1 Inoculate 3 portions of Inner Mongolian cheese into 10% skim milk medium at 37°C according to 1% inoculation amount, and culture them at 37°C. After curdling, perform 10-fold gradient dilution, and then dilute to 10% in turn. -8 gradient. Select 4 appropriate dilutions and take 1mL and mix with MRS solid medium according to the pouring method and pour it onto the plate. Cultivate in an anaerobic tank at 37°C for 48 hours, carefully observe the colony shape, select colonies of different shapes and separate them by streaking on the MRS solid medium again, repeat the streaking and separation for 3 generations, and obtain the purified str...

Embodiment 2

[0063] Physiological and biochemical characteristics of embodiment 2 Lactobacillus rhamnosus NJ551

[0064] 1. Gram-positive bacteria, short rods (0.5-1.2μm X 3.0-10.0μm), with different traits under different nutritional conditions, mainly chain-like and cross-arranged under microscope conditions ( figure 1 ).

[0065] like figure 1 Shown: Microscopic image of Lactobacillus rhamnosus NJ551.

[0066] 2. Tolerance in artificial gastric acid and artificial small intestine fluid

[0067] Lactobacillus rhamnosus NJ551 has good resistance to artificial gastric acid and artificial small intestinal juice, the 2h survival rate is greater than 75% in 37°C pH 3.0 artificial gastric juice, and the 6h survival rate is greater than 50%; in 37°C pH 7.0 artificial gastric juice The 1h survival rate in the artificial small intestine fluid is greater than 26%, and the 2h survival rate is greater than 13% (see figure 2 ). Demonstrates a strong potential to reach the colon alive.

[0068]...

Embodiment 3

[0071] Example 3 Bacillus coagulans BC01

[0072] Preparation of live bacterial preparation of Bacillus coagulans BC01

[0073] 1. Collect samples that may contain Bacillus coagulans. The samples come from soil, fermented food, plants, human intestines, etc., and then place the samples in sterilized sterile bottles, and then adopt the following two methods to obtain pure strains :

[0074] 1.1 Obtaining pure strains Method 1: Add bacteria-containing samples and 100mL sterile saline to a sterile bottle to prepare a bacteria-containing stock solution, and then serially dilute the stock solution by 10 1 times, 10 2 times, 10 3 times, 10 4 times, 10 5 times, 10 6 times, 10 7 times, 10 8 times, 10 9 times. Spread the above stock solution and the dilutions of each dilution factor on the solid medium for enriching Bacillus coagulans, incubate in an incubator at 40°C for 48 hours, and select a single colony with sufficient separation and good growth and inoculate it again in...

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Abstract

The invention provides a probiotic composition beneficial to improvement of immunity. The composition comprises lactobacillus rhamnosus NJ551 and bacillus coagulans BC01, wherein the lactobacillus rhamnosus NJ551 strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M2016157; and the bacillus coagulans BC01 strain is preserved in China center fortype culture Collection, and the preservation number is CCTCC NO: M2017813. The probiotic composition provided by the invention can realize or exceed the effects of restoring or improving the intestinal mucosa permeability, NK cell killing rate and serum gamma-interferon (IFN-gamma) and TNF-alpha content of mice, which can be realized by single bacteria at a relatively large intake amount. The probiotic composition provided by the invention is beneficial to improvement of immunity.

Description

technical field [0001] The invention belongs to microorganisms, in particular to a probiotic composition. Background technique [0002] Immunity is the body's ability to resist disease and maintain its own health. It is the immune system that performs this function in the human body. The immune system includes immune organs, immune tissues, and immune molecules, which interact to form a huge body defense network. Although humans have not yet fully understood the immune system, existing knowledge has proved that 70% of the immune cells in the human body gather in the intestines, and the development of the immune system is closely related to the intestinal flora. [0003] On the intestinal mucosa of the human body, there are thousands of intestinal flora with the number reaching 100 billion. These intestinal flora and the mucosal immune system of the human body have reached a dynamic balance. On the one hand, the mucosal system of the human body adjusts and maintains the qu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23L33/135A61K35/742A61K35/747A61P37/04C12R1/225C12R1/07
CPCC12N1/20A23L33/135A61K35/742A61K35/747A61P37/04A23V2002/00C12R2001/225C12N1/205A23V2400/175A23V2200/324A61K2300/00
Inventor 喻扬郁雪平吴婷婷
Owner THANKCOME BIOLOGICAL SCI & TECH CO LTD
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