Apparatus and method for analyzing interactions between pollutants and single particles

Abstract

A device and method for analyzing the interaction between pollutants and single particles. The device includes a light source unit for providing a light source; a microscope unit with an objective lens on it; a sensing unit set above the objective lens; On the unit, it is used to hold the single particle solution; the electric field unit provides the electric field for the single particle solution to generate vibration; and the signal acquisition unit collects the outgoing light signal passing through the microscope unit. The invention utilizes the imaging advantages of single particles, which can collect and quickly analyze the interaction with pollutants in real time; does not require rivets for single particles to prevent particle escape, and is easy to operate; does not require background modification, and the ITO-loaded glass sheet is used as a sensor chip It can effectively shield background signals and reduce background interference; alternating current can suppress the disordered Brownian motion of particles to achieve high-sensitivity detection.

Description

technical field [0001] The invention belongs to the field of environmental science and technology, and relates to a device and method for analyzing the interaction between pollutants and single particles. Background technique [0002] With the continuous development of nanotechnology, nanomaterials are widely used, and the nanoparticles in them are inevitably discharged into the environment and accumulate continuously. Nanoparticles can be the source and sink of pollutants, and can interact with pollutants in the environment (such as heavy metal ions, sulfide, bisphenol A, etc.), which will largely affect the migration and transformation process of nanoparticles, and then affect the its potential ecotoxicity. Therefore, the development of a new method for analyzing the interaction between nanoparticles and pollutants in the water environment is not only of great significance for understanding the interaction process and mechanism of the two, but also has a certain theory fo...

AI Technical Summary

Problems solved by technology

[0003] In the study of the interaction between nanomaterials and pollutants, traditional experimental methods include analyzing the ability of nanomaterials to directly adsorb pollutants, and using quartz crystal microbalances or surface plasmon resonance spectrometers to study the interaction between particles and pollutants. These methods not only Time-consuming and labor-intensive and only focus on the overall performance of nanomaterials, ignoring the information of nanomaterial heterogeneity

Due to the lack of spatial resolution using traditional methods, most of these studies focus on the nanoparticle population level, and it is impossible to accurately obtain information on the behavioral differences between individual nanoparticles, so it is difficult to understand the interaction and morphology of nanoparticles and surface molecules. process of difference

In recent years, the development of optical microscopy techniques such as fluorescence microscopy and atomic force microscopy (AFM) can achieve single-particle imaging at the nanometer scale, but fluorescence microscopy can only test molecules with fluorescent signals or fluorescently label molecules, which may affect The measurement of molecular binding processes greatly limits the scope of application of this technique

AFM needs to immobilize a single nanoparticle on the tip of the probe, and scan the probe to obtain information on molecular interactions, but this technology cannot achieve high-throughput measurement, and the operation is time-consuming and expensive.

[0004] Due to the size effect of single particles, difficulty in detection, and active Brownian motion in aqueous solution, there are still great challenges in the study of the interaction between single particles and pollutants with existing technologies and methods.

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