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Handheld leukocyte counter and leukocyte counting method

A white blood cell and counting instrument technology, applied in instruments, scientific instruments, and material analysis through optical means, can solve the problems of not being suitable for emergency medical diagnosis, incomplete lysis of red blood cells, and short detection time window. Lightweight, accurate counting, small size effect

Pending Publication Date: 2020-11-10
苏州中科苏净生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the hemolysis counting method adopted by HemoCue has the following disadvantages: first, the method uses the transmission absorption method, which is easily interfered by impurities; second, the detection time window is short, and the detection must be strictly controlled within 2-10 minutes, and the red blood cell lysis is too short Incomplete, some white blood cells will be dissolved if the time is too long
The above shortcomings lead to low detection accuracy of the method
[0009] The above methods are all common white blood cell counting methods, but they are not suitable for primary medical care, mobile medical care, and emergency rescue medical diagnosis in special environments due to the professional requirements for operators, equipment volume, price, and maintenance during use. There are great limitations in the application process

Method used

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  • Handheld leukocyte counter and leukocyte counting method
  • Handheld leukocyte counter and leukocyte counting method
  • Handheld leukocyte counter and leukocyte counting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Figure 7A-Figure 7C It is an example of the structure of the detection card of the present invention, which is in the form of a slit microfluidic chip structure. Figure 7A - Figure 7CIn the first shown embodiment, the detection card includes a support substrate 21 and a sampling and detection cavity 22 arranged on the support substrate 21, wherein the support substrate 21 is the hand-held part of the microfluidic chamber, which is designed to be suitable for hand-held Shape, as in the first embodiment, the handle is rectangular, and the front end is extended with a sampling detection chamber 22 with arc-shaped edges; front end. The sampling detection chamber 22 is a semi-open cavity formed by two parallel chamber side walls 24 with a certain gap, including a sampling port 27, a sampling notch 29, a detection area 25, and a flow diversion connecting the sampling port 27 and the detection area 25 Tank area 26, wherein:

[0073] The sampling port 27 is located at th...

Embodiment 2

[0085] The detection card of this embodiment is the same as that of Embodiment 1, and the dyeing agent is SYTO-9.

[0086] The human-computer interaction module mainly includes a display screen and function keys to complete the input of user instructions and the output of detection results; the function keys include on-off key, detection key and return key. Start the detection program, the return key is used to return to the main page.

[0087] The main control unit 8 is responsible for the control of the working process of the instrument, processing and analysis of the collected images, and selects the 4412 core control board of the friendly arm, which is electrically connected with the function keys and the display screen on the human-computer interaction module, and connected with the photoelectric detection The modules are electrically connected, and after the human-computer interaction module sends instructions to the main control unit 8, the main control unit 8 controls ...

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Abstract

The invention discloses a handheld leukocyte counter and a leukocyte counting method. The handheld leukocyte counter comprises a handheld shell, a photoelectric detection module, a main control unit,a man-machine interaction module, a detection card cabin door, a bracket, a detection card and the like, wherein the photoelectric detection module, the main control unit, the man-machine interactionmodule, the detection card cabin door, the bracket, the detection card and the like are assembled together, and the detection card is of a slit micro-fluidic chip structure and counts leukocytes according to the fluorescence excitation principle. The counter is small in size, light in weight and convenient to carry and place; the counting method can avoid interference of impurities; counting is accurate, precision is high, detection operation is easy, detection efficiency is high, and the counter is particularly suitable for leukocyte detection in primary medical treatment, mobile medical treatment and emergency rescue medical diagnosis in special environments.

Description

technical field [0001] The invention relates to the technical field of blood analysis and detection, in particular to a hand-held white blood cell counting instrument and a white blood cell counting method. Background technique [0002] There are three types of blood cells in the human body: white blood cells, red blood cells, and platelets. White blood cells take on many important tasks in the human body. They can swallow foreign bodies and produce antibodies, heal the body's damage, resist the invasion of pathogens, and develop immune resistance to diseases. Under normal circumstances, the total number of white blood cells in the human body is relatively stable, but inflammation or other diseases will cause changes in the number of white blood cells, which are often manifested by significant changes in the number of white blood cells. In other words, inflammation or other diseases in the body can cause changes in the total number of white blood cells, so the change in val...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14G01N21/64
CPCG01N15/1434G01N15/1484G01N21/6428G01N21/6486G01N2015/1006G01N2015/144G01N2015/1486
Inventor 李悦琴
Owner 苏州中科苏净生物技术有限公司
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