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Leukocyte counter and leukocyte counting method

A white blood cell and counting instrument technology, applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of being unsuitable for grassroots medical and health institutions, incomplete lysis of red blood cells, and short detection time window, etc., to achieve high detection efficiency, accurate counting, light weight effect

Pending Publication Date: 2020-11-10
苏州中科苏净生物技术有限公司
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  • Claims
  • Application Information

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Problems solved by technology

However, the hemolysis counting method adopted by HemoCue has the following disadvantages: first, the method uses the transmission absorption method, which is easily interfered by impurities; second, the detection time window is short, and the detection must be strictly controlled within 2-10 minutes, and the red blood cell lysis is too short Incomplete, some white blood cells will be dissolved if the time is too long
The above shortcomings lead to low detection accuracy of the method
[0009] The above methods are common methods for counting the total number of white blood cells, but they are not suitable for the needs of grassroots medical and health institutions and mobile medical care in terms of professional requirements for operators, equipment volume, price, maintenance, etc., and there are many problems in the application process. big limitations

Method used

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  • Leukocyte counter and leukocyte counting method
  • Leukocyte counter and leukocyte counting method
  • Leukocyte counter and leukocyte counting method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Figure 7A-Figure 7C It is an example of the structure of the detection card of the present invention, which is in the form of a slit microfluidic chip structure. Figure 7A - Figure 7C In the first shown embodiment, the detection card includes a support substrate 21 and a sampling and detection cavity 22 arranged on the support substrate 21, wherein the support substrate 21 is the hand-held part of the sandwich microfluidic chip, which is designed to be suitable for hand-held Shape, as in the first embodiment, the handle is rectangular, and the front end is extended with a sampling detection cavity 22 with arc-shaped edges; the sampling detection cavity 22 can be integrally formed with the support substrate 21, or the sampling detection cavity 22 can be bonded to front end. The sampling detection chamber 22 is a semi-open cavity formed by two parallel chamber side walls 24 with a certain gap, including a sampling port 27, a sampling notch 29, a detection area 25, an...

Embodiment 2

[0083] The detection card of this embodiment is the same as that of Embodiment 1, and the dyeing agent is SYTO-9.

[0084] Human-computer interaction module II mainly includes a display screen and function keys to complete the input of user instructions and the output of test results; the function keys include on-off key, test key and return key. to start the detection program, the return key is used to return to the main page.

[0085] The main control unit 8 is responsible for the control of the working process of the instrument, processing and analysis of the collected images, and selects the 4412 core control board of the friendly arm, which is electrically connected with the function keys and the display screen on the human-computer interaction module, and connected with the photoelectric detection The modules are electrically connected, and after the human-computer interaction module sends instructions to the main control unit 8, the main control unit 8 controls the on o...

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Abstract

The invention discloses a leukocyte counter and a leukocyte counting method. The leukocyte counter comprises a human-computer interaction module, a photoelectric detection module, a main control unitand a detection card, wherein the detection card is arranged in the photoelectric detection module, and the main control unit is electrically connected with the man-machine interaction module and thephotoelectric detection module. The leukocyte counter has the characteristics that (1) the leukocyte counter is small in size, light in weight and convenient to carry and place; (2) a fluorescence excitation method is adopted as a working principle, so the interference of impurities is avoided, counting is accurate, and precision is high; (3) the operation is simple during detection, and the detection efficiency is high; and the deficiency of leukocyte detection instruments in existing primary medical treatment, mobile medical treatment and emergency rescue medical diagnosis in special environments can be made up.

Description

technical field [0001] The invention relates to the technical field of blood analysis and detection, in particular to a white blood cell counting instrument and a white blood cell counting method. Background technique [0002] There are three types of blood cells in the human body: white blood cells, red blood cells, and platelets. White blood cells take on many important tasks in the human body. They can swallow foreign bodies and produce antibodies, heal the body's damage, resist the invasion of pathogens, and develop immune resistance to diseases. Under normal circumstances, the total number of white blood cells in the human body is relatively stable, but inflammation or other diseases will cause changes in the number of white blood cells, which are often manifested by significant changes in the number of white blood cells. In other words, inflammation or other diseases in the body can cause changes in the total number of white blood cells, so the change in value can be ...

Claims

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Application Information

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IPC IPC(8): G01N15/14
CPCG01N15/1436G01N2015/1006
Inventor 李悦琴
Owner 苏州中科苏净生物技术有限公司
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