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Two-dimensional hyperbranched polyanion nanosheet modified nanofiber scaffold and preparation method and application thereof

A nanofiber, fiber technology, applied in the direction of fiber type, fiber treatment, pharmaceutical formulation, etc., to achieve broad application prospects, efficient dryness, and the effect of maintaining dryness

Active Publication Date: 2020-11-06
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there have been many reports on the combined culture of stem cells and nanofibrous materials, but these nanofibrous materials are still insufficient in promoting the adhesion and proliferation of stem cells, inhibiting premature undirected differentiation of stem cells, and maintaining the stemness of stem cells.

Method used

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  • Two-dimensional hyperbranched polyanion nanosheet modified nanofiber scaffold and preparation method and application thereof
  • Two-dimensional hyperbranched polyanion nanosheet modified nanofiber scaffold and preparation method and application thereof
  • Two-dimensional hyperbranched polyanion nanosheet modified nanofiber scaffold and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1: Preparation of polycaprolactone fibers (PCL-GO-HPGS) coated with GO-HPGS nanosheets

[0055] 1. Preparation and characterization of GO-HPGS 2D nanosheets (1.1) Azidated hyperbranched polyglycerol (HPG-N 3 )Synthesis

[0056] First, referring to the literature (Advanced Materials 2014, 26 (17), 2688-2693; Angewandte Chemie International Edition 2014, 53 (43), 11650-11655) to synthesize hyperbranched polyglycerol (HPG) by one-step ring-opening anionic polymerization (ROAP) ) crude product, then the crude HPG product was dissolved in methanol and dialyzed for 3 days for further purification. Centrifuge to remove insoluble precipitates, take the supernatant and concentrate, and dry at 60°C overnight under high vacuum to remove residual methanol to obtain hyperbranched polyglycerol (HPG), molecular weight = 7200g.mol -1 , PDI<1.2, branching degree 50%.

[0057] HPG (3.5 g) was dried overnight at 60° C. under high vacuum (HV). The dried HPG was then dissolved i...

Embodiment 2

[0073] Example 2: Preparation of polycaprolactone fibers (PCL-GO-HPGS / 2) coated with GO-HPGS nanosheets

[0074] Using the same method as in Example 1 to prepare PCL fibers and GO-HPGS nanosheets, and then use O 2 The PCL fibers obtained in the previous step were treated with plasma for 0.5 min to generate hydrophilic groups and convert the surface of the hydrophobic polymer to hydrophilic, which facilitated covalent bonding with GO-HPGS nanosheets. then O 2 After plasma treatment, the PCL fibers were soaked in the GO-HPGS nanosheet dispersion (the concentration was 0.5 mg / mL, the solvent was H 2 O), soaked for 0.5min, and then removed to obtain coated GO-HPGS nanosheets, dried under vacuum overnight to obtain GO-HPGS nanosheets coated PCL fiber, named PCL-GO-HPGS / 2.

Embodiment 3

[0075] Example 3: Preparation of polycaprolactone fibers (PCL-GO-HPGS / 3) coated with GO-HPGS nanosheets

[0076] Using the same method as in Example 1 to prepare PCL fibers and GO-HPGS nanosheets, and then use O 2 The PCL fibers obtained in the previous step were treated with plasma for 3 min to generate hydrophilic groups and convert the surface of the hydrophobic polymer to hydrophilic, which facilitated covalent bonding with GO-HPGS nanosheets. then O 2 After plasma treatment, the PCL fibers were soaked in the GO-HPGS nanosheet dispersion (the concentration was 3 mg / mL, the solvent was H 2 (0), soak for 5min, and then take it out to obtain the coated GO-HPGS nanosheets, and dry them overnight under vacuum to obtain PCL fibers coated with GO-HPGS nanosheets, named as PCL-GO-HPGS / 3.

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Abstract

The invention discloses a two-dimensional hyperbranched polyanion nanosheet modified nanofiber scaffold and a preparation method and application thereof. A nanofiber composite (PCL-GO-HPGS) is specifically provided, and is a material obtained after the surfaces of polycaprolactone fibers are coated with hyperbranched polyglycerol surface modified graphene oxide. By using the nanofiber composite material as the scaffold, the adhesion and proliferation performance of IPS cells can be improved, the dryness of the IPS cells can be efficiently maintained, and premature non-directional differentiation is avoided. In the neural differentiation phase, the PCL-GO-HPGS scaffold is capable of promoting directional differentiation of embryoids formed by the IPS cells to neuronal cells and simultaneously inhibiting differentiation of the IPS cells to astrocytes. The PCL-GO-HPGS nanofiber composite material can be used as the novel nanofiber scaffold with hyperbranched molecular multivalent bonds and capable of promoting bio-adhesive performance, and has a wide application prospect in treatment of diseases such as stem cell differentiation and nerve tissue regeneration.

Description

technical field [0001] The invention belongs to the field of tissue engineering scaffolds, and in particular relates to a nanofiber scaffold modified by two-dimensional hyperbranched polyanion nanosheets, a preparation method and application thereof. Background technique [0002] Neurodegenerative diseases, including Parkinson's disease, Alzheimer's disease, and amyotrophic lateral sclerosis, are characterized by idiopathic neuronal loss in different parts of the central nervous system. A series of corresponding dysfunctions. The use of human embryonic stem cells for cell replacement therapy has aroused great interest, but some ethical issues have hindered the development of this research. Studies have found that by introducing specific transcription factors, somatic cells can be induced into cells with the characteristics of embryonic stem cells, that is, induced pluripotent stem cells (IPS cells), and obtaining human IPS cells does not involve obvious ethical issues. And...

Claims

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Application Information

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IPC IPC(8): D06M11/74D06M10/06A61L27/38A61L27/34A61L27/08D06M101/32
CPCA61L27/08A61L27/34A61L27/383A61L27/3834A61L2400/12D06M10/06D06M11/74D06M2101/32
Inventor 邱逦程冲夏懿曹素娇吴熙政李爽马田周密马朗
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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