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A method for detecting the toxicity of nanoplastics

A technology of nanoplastics and detection methods, which is applied in material inspection products, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problem that the influence of nanoplastics on aquatic phytoplankton metabolites has not been well elucidated and understood, etc. problem, to achieve the effect of short experimental period

Active Publication Date: 2022-07-15
UNIV OF SCI & TECH OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As far as we know, there are currently studies on the effects of nano-metal materials and nano-carbon materials on the metabolism of aquatic organisms, but the effects of nano-plastics on aquatic phytoplankton metabolites have not been well elucidated and understood

Method used

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  • A method for detecting the toxicity of nanoplastics
  • A method for detecting the toxicity of nanoplastics
  • A method for detecting the toxicity of nanoplastics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0113] The test organism was Microcystis aeruginosa, cultivated in a lighted incubator.

[0114] The nano-polystyrene is prepared by an emulsion polymerization method, and the concentration of the mother liquor is 43 g / L. Dilute to obtain nano-polystyrene of 0mg / L~100mg / L respectively.

[0115] After exposure of Microcystis aeruginosa to the above-mentioned different concentrations of nano-polystyrene for 96h, the measurement indicators of Microcystis aeruginosa are as follows:

[0116] Growth inhibition rate: Microcystis aeruginosa was exposed to nano-polystyrene for 96h, samples were collected, and the growth inhibition rate of the test group was analyzed by detecting the cell density. The growth inhibition rate is calculated according to the difference percentage of the cell density between the test group and the control group; the determination of the cell density is as follows: Microcystis aeruginosa is exposed to nano-polystyrene for 96h, and the cell density is measure...

Embodiment 2

[0124] The test organism was Microcystis aeruginosa, cultivated in a lighted incubator.

[0125] The nano-polystyrene is prepared by an emulsion polymerization method, and the concentration of the mother liquor is 43 g / L. Dilute to obtain nano-polystyrene of 0mg / L~100mg / L respectively.

[0126] After exposure of Microcystis aeruginosa to different concentrations of nano-polystyrene for 96h, the measurement indicators of Microcystis aeruginosa are as follows:

[0127] Chlorophyll a: Microcystis aeruginosa was exposed to nano-polystyrene for 96h, the samples were collected, and chlorophyll a was extracted by alcohol extraction.

[0128] Chlorophyll a test

[0129] When the exposure experiment was carried out for 96 h, 1 mL of the sample was collected, centrifuged at 4°C, the rotation speed was 10000 g, and the time was 10 min. Discard the supernatant and resuspend in 1 mL of 95% methanol in water. After 5 min in a 60°C water bath, centrifuge again at 4°C at a speed of 3000 g...

Embodiment 3

[0137] The test organism was Microcystis aeruginosa, cultivated in a lighted incubator.

[0138] The nano-polystyrene is prepared by an emulsion polymerization method, and the concentration of the mother liquor is 43 g / L. Dilute to obtain nano-polystyrene of 0mg / L~100mg / L respectively.

[0139] After exposure of Microcystis aeruginosa to different concentrations of nano-polystyrene for 96h, the measurement indicators of Microcystis aeruginosa are as follows:

[0140] Phycocyanin: Microcystis aeruginosa was exposed to nano-polystyrene for 96h, samples were collected, and phycocyanin was measured by detecting the fluorescence intensity.

[0141] Phycocyanin Experiment

[0142] When the exposure experiment was carried out for 96 hours, 200 μL was transferred to a black microplate, and the autofluorescence of natural pigments (Ex: 590nm / Em: 650nm) was detected by a microplate reader.

[0143] The result is as Figure 4 said, by Figure 4 It can be seen that the fluorescence i...

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Abstract

The invention provides a method for detecting the toxicity of nano-plastics, which comprises the following steps: after the microcystis aeruginosa is pre-cultured in a medium, it is exposed to the nano-plastics to be tested; The concentration of nanoplastics was judged by protein fluorescence intensity, intracellular microcystin, extracellular microcystin and / or metabolites of Microcystis aeruginosa; control group included water, culture medium and Microcystis aeruginosa; test group Including culture medium, Microcystis aeruginosa and nanoplastics to be tested. In the present invention, the growth inhibition rate of Microcystis aeruginosa, the content of chlorophyll a, the fluorescence intensity of phycocyanin, the production and release of microcystins and / or the changes of metabolites of Microcystis aeruginosa are used to evaluate the effect of nanoplastics on Microcystis aeruginosa. growth and / or toxic effects of metabolites. By detecting physiological and biochemical indicators, it can be preliminarily judged whether the sample to be tested is contaminated by nanoplastics, which provides a new method for the toxicological detection of nanoplastics.

Description

technical field [0001] The invention relates to the technical field of toxicity detection methods, in particular to a detection method for the toxicity of nano-plastics. Background technique [0002] Due to the widespread use of plastics, substantial enrichment has occurred in coastal areas and in the oceans, leading to growing concerns about the hazards of aquatic life. Due to the degradation of additives and plastics in some consumer products, microplastics of ≤5mm will be formed, which will cause water pollution once released into the water body. In the environment, microplastics may degrade into nanoplastics. Therefore, the existence, fate and pollution of nanoplastics in the environment have also received extensive attention in recent years. Nanopolystyrene is a commonly used nanoplastic particle with a wide range of applications including biosensors, personal care products, drug delivery and bioimaging. At present, studies have shown that nano-polystyrene has entero...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02G01N33/68G01N30/02G01N27/62G01N21/64
CPCC12Q1/02G01N33/68G01N30/02G01N27/62G01N21/6428
Inventor 童中华马喜文
Owner UNIV OF SCI & TECH OF CHINA
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