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Antibacterial peptide LJ-2 and application thereof

An LJ-2, antimicrobial peptide technology, applied in the field of antimicrobial peptide research, can solve the problems of unsatisfactory safety or activity, large workload, long time consumption, etc., and achieve good heat resistance, strong antibacterial activity, and low hemolysis. Effect

Active Publication Date: 2020-10-30
HENAN INST OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The isolation and identification of organisms can obtain many antimicrobial peptides from natural sources, but there are disadvantages such as time-consuming, heavy workload, low yield, and the safety or activity of most peptides is not ideal; the artificial design and transformation of antimicrobial peptides is the key to obtaining antimicrobial peptides The rapid and effective way has become an important direction for the development and research of antimicrobial peptides

Method used

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  • Antibacterial peptide LJ-2 and application thereof
  • Antibacterial peptide LJ-2 and application thereof
  • Antibacterial peptide LJ-2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The synthesis of antimicrobial peptide LJ-2 of the present invention is as follows:

[0032] The antimicrobial peptide LJ-2 of the present invention is synthesized by an automatic polypeptide synthesizer according to the conventional solid-phase chemical synthesis method of polypeptides, and the synthesis direction is from the C-terminal to the N-terminal, and the detailed steps of its synthesis are as follows:

[0033] a. Swelling resin: add Fmoc-Leu-Wang resin resin into the reactor of automatic peptide synthesizer, and add dimethylformamide DMF to swell. The amount of DMF added to 1g Fmoc-Leu-Wang resinin resin is about 12mL (DMF should be completely immersed in the resin), the swelling time is 10-30 minutes, and the swelling can be repeated 1-2 times;

[0034] B, deprotection: after resin swelling, 20% piperidine (20% piperidine is that piperidine is dissolved in dimethylformamide DMF and is prepared by dissolving piperidine in dimethylformamide DMF) in the storage ...

Embodiment 2

[0044] A kind of antimicrobial peptide LJ-2, its amino acid sequence is as follows:

[0045] Arginine-Arginine-Valine-Leucine-Arginine-Isoleucine-Leucine-Lysine-Histidine-Histidine-Glutamine-Glycine-Leucine - Isoleucine - Arginine - Asparagine - Leucine - Leucine (SEQ ID NO. 1).

[0046] The antimicrobial peptide LJ-2 contains 18 amino acid residues, its molecular weight is 2235.78Da, and its isoelectric point is 12.6. The antimicrobial peptide LJ-2 is positively charged and can attract negative charges on the surface of bacteria.

[0047] The antimicrobial peptide LJ-2 in this example is synthesized by using an automatic peptide synthesizer according to the conventional solid-phase chemical synthesis method of peptides, and the finally synthesized antimicrobial peptide LJ-2 is purified and analyzed by a semi-preparative high-performance liquid chromatograph, and its purity is ≥ 95%. .

[0048] Identification of the gained antimicrobial peptide LJ-2 product of the present i...

Embodiment 3

[0054] This embodiment is the performance analysis of antimicrobial peptide LJ-2:

[0055] 3.1 Antibacterial activity analysis of antimicrobial peptide LJ-2

[0056] The antibacterial activity of antimicrobial peptide LJ-2 was determined by the agar plate diffusion method, and the tested microbial strains were: Escherichia coli, Salmonella, Staphylococcus aureus and Candida albicans, Escherichia coli CVCC1568 and Salmonella CVCC3776 were purchased from China Veterinary Microbiology Strain Preservation Management Center, Escherichia coli CVCC8099, Staphylococcus aureus ATCC25923 and Candida albicans ATCC10231 were purchased from Nanjing Bianyi Biotechnology Co., Ltd. Clinical drug-resistant strains of Escherichia coli were isolated and identified by themselves (Li Jie, Hang Bolin, Dong Mengmeng , et al. Isolation and identification of drug-resistant Escherichia coli from pigs [J]. China Pig Industry, 2018,13(12):42-46,50).

[0057] The above-mentioned test microorganisms (1×10...

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Abstract

The invention discloses an antibacterial peptide LJ-2 and an application thereof. The amino acid sequence of the antibacterial peptide LJ-2 is as follows: the amino acid sequence of the antibacterialpeptide LJ-2 is shown in the specification: arginine, arginine, valine, leucine, arginine, isoleucine, leucine, lysine, histidine, histidine, glutamine, glycine, leucine, isoleucine, arginine, asparagine, leucine and leucine; the antibacterial peptide LJ-2 has good antibacterial activity on gram-positive bacteria, gram-negative bacteria and fungi, and has the advantages of low hemolysis, heat resistance, salt resistance, acid and alkali resistance and repeated freeze thawing resistance. Therefore, the antibacterial peptide LJ-2 disclosed by the invention has a wide application prospect in thefields of anti-infective drug preparation, animal husbandry development, human disease prevention and treatment, food preservation, detergents, cosmetics and the like.

Description

technical field [0001] The invention belongs to the technical field of antimicrobial peptide research, and in particular relates to an antimicrobial peptide LJ-2 and its application. Background technique [0002] Antibiotics Antibacterial drugs are the most commonly used drugs for the treatment of infectious diseases in humans and animals. The application of this drug has significantly improved the quality of life of humans and saved the lives of countless people and animals. However, with the abuse of traditional antibiotics, the cycle of bacterial resistance to antibiotics has been continuously shortened, resulting in the proliferation of multiple drug-resistant bacteria in recent years. Due to the accelerating rate of bacterial resistance and mutation, the development of new antibiotics is very slow. At present, serious bacterial resistance has increased the difficulty of preventing and controlling human and animal diseases, and has become a major problem that endangers ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08A61K38/10A61P31/04A61P31/10
CPCC07K7/08A61P31/04A61P31/10A61K38/00Y02A50/30
Inventor 杭柏林李杰刘保国胡建和徐彦召王磊王秋霞张慧辉杨雪峰宁春妹
Owner HENAN INST OF SCI & TECH
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