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Construction method of traumatic brain injury animal model

An animal model and construction method technology, applied in animal husbandry and other directions, can solve the problems of different emphases, difficult to control and grasp the amount of injury, and increase the difficulty of animal models of brain injury, so as to achieve good repeatability and little influence of individual differences. Effect

Inactive Publication Date: 2020-10-27
重庆市第七人民医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] It has become the goal of most basic and clinical workers to recognize and understand the mechanism of brain injury and the pathophysiological changes of brain tissue after injury, and to formulate reasonable and effective treatment measures on this basis. The establishment of experimental animal models has become an important research method. One of the components, the existing brain trauma models mainly include: free fall impact model (single damage mechanism, simple method, easy to control conditions, consistent injury degree, used to study the molecular mechanism of brain damage), hydraulic impact injury model ( The hydraulic impact injury model is used to study the pathology and physiology and pharmacology of brain injury), and the negative pressure brain injury model (simply studying accelerated injury, ignoring the effects of other factors, does not fully conform to the actual situation, and there are large differences between individuals) , compressed air injury model, acceleration injury and deceleration injury model, and mechanical oscillation model; but the occurrence of brain injury is not single, and often multiple types of injury coexist, and a single type of brain injury is rare, so The injury mechanism of some injury models is still not completely clear, and the amount of injury is difficult to control, which increases the difficulty of establishing animal models of brain injury
At present, the animal models established by various methods have different application emphases, but there is still no generally recognized and relatively complete method that can produce various types of brain injury animal models

Method used

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  • Construction method of traumatic brain injury animal model

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A construction method of an animal model of traumatic brain injury, the construction method comprising the following steps:

[0027] 1) Select normal rats or mice, fast for 12 hours before the operation, drink water freely, and anesthetize the experimental animals with 1.5g / L pentobarbital sodium at 50ml / kg before the operation;

[0028] 2) Inject protective agents into experimental animals and animal models of traumatic brain injury before operation;

[0029] 3) Fix the head of the rat on the stereotaxic head frame in the prone position;

[0030] 4) Prepare the head skin, perform routine disinfection, cut the scalp about 2 cm along the midline, and use a 1.5 mm drill bit of a dental desktop electric drill to drill the skull between the anterior and posterior fontanelles, 2.5 cm away from the left and right side of the midline, and use this as the center Use mosquito forceps to open a circular bone window with a diameter of 5mm, keep the dura mater intact, and aim at t...

Embodiment 2

[0052] A method for constructing an animal model of traumatic brain injury, using a protective agent as a variable, comprising the following steps:

[0053] Select normal rats or mice, fast for 12 hours before the operation, drink water freely, and use 1.5g / L pentobarbital sodium to anesthetize the experimental animal by intraperitoneal injection of 50ml / kg before the operation;

[0054] Inject protective agents into experimental animals and animal models of traumatic brain injury before operation;

[0055] The step 2) protective agent is butylphthalide, glycine, dexmedetomidine, α-linolenic acid, ulinastatin, curcumin, puerarin, vitamin D, breviscapine, ginsenoside, stilbene glycoside , Muscone in one or more compounds, through intravenous injection to experimental animals.

[0056] The head of the rat was fixed on the stereotaxic head frame in the prone position, and the animal model was prepared with reference to the above-mentioned examples;

[0057] The bone window of t...

Embodiment 3

[0067] A construction method of an animal model of traumatic brain injury, the construction method comprising the following steps:

[0068] Select normal rats or mice, fast for 12 hours before the operation, drink water freely, and use 1.5g / L pentobarbital sodium to anesthetize the experimental animal by intraperitoneal injection of 50ml / kg before the operation;

[0069] Inject protective agents into experimental animals and animal models of traumatic brain injury before operation;

[0070] The head of the rat was fixed on the stereotaxic head frame in the prone position;

[0071] Use a rubber hammer, a standard pendulum, or a percussion hammer to directly hit the skull of the experimental animal fixed on the stereotaxic head frame to form a closed brain injury.

[0072] Quickly inject a certain amount of normal saline or the above-mentioned protective agents into the cranial cavity of the experimental animal fixed on the stereotaxic head frame.

[0073] Injectable protectiv...

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Abstract

The invention discloses a construction method of a traumatic brain injury animal model, and relates to the technical field of brain injury animal models. The construction method comprises the following steps: selecting a normal rat or mouse, and performing intraperitoneal injection anesthesia on the selected normal rat or mouse; injecting a protective agent into an experimental animal and the traumatic brain injury animal model before operation; and constructing the traumatic brain injury animal model under different conditions and methods. The protective agent is injected into the experimental animal while the brain injury animal model is constructed, the brain injury animal model is more similar to a clinically generated brain injury mechanism, the application range is relatively wide, the influence of individual difference is small, the repeatability is good, and a basis can be provided for clinically searching corresponding treatment means and measures.

Description

technical field [0001] The invention relates to the technical field of brain injury animal models, in particular to a method for constructing traumatic brain injury animal models. Background technique [0002] Traumatic brain injury (traumatic brain injury, TBI) is one of the major diseases that threaten human life today, and the mortality rate of patients with severe traumatic brain injury is very high, about 30%-50%. In my country, traumatic brain injury has become an important disease that threatens people's lives due to the obvious increase in the incidence of traffic accident injuries and fall injuries in recent years. [0003] Brain injury mainly refers to primary injuries such as concussion, diffuse axonal injury, brain contusion, and brain contusion, as well as secondary changes such as intracerebral hematoma, cerebral edema, encephalomalacia, and brain herniation. Craniocerebral injury is mainly related to the location, size, speed and degree of extrusion of violen...

Claims

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Application Information

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IPC IPC(8): A01K67/027
CPCA01K67/027A01K2207/20A01K2207/30A01K2227/105A01K2267/0356
Inventor 鲁宏陈建强战跃福
Owner 重庆市第七人民医院
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