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Fluorescent probe for detecting Cu<2+> and detecting HPO4<2-> by utilizing Cu<2+> as well as preparation method and application of fluorescent probe

A technology of fluorescent probes and probes, applied in the direction of fluorescence/phosphorescence, chemical instruments and methods, luminescent materials, etc., can solve problems such as interference and fewer fluorescent probes, and achieve simple methods, high sensitivity, strong sensitivity and resistance The effect of interference ability

Active Publication Date: 2020-10-16
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The present invention is to solve the existing detection HPO42– fewer fluorescent probes, and is subject to H2PO4- interference caused technical problems, but provide a method to detect Cu2+ and can Fluorescent probe for detecting HPO42– using Cu2+ and its preparation method and application

Method used

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  • Fluorescent probe for detecting Cu&lt;2+&gt; and detecting HPO4&lt;2-&gt; by utilizing Cu&lt;2+&gt; as well as preparation method and application of fluorescent probe
  • Fluorescent probe for detecting Cu&lt;2+&gt; and detecting HPO4&lt;2-&gt; by utilizing Cu&lt;2+&gt; as well as preparation method and application of fluorescent probe
  • Fluorescent probe for detecting Cu&lt;2+&gt; and detecting HPO4&lt;2-&gt; by utilizing Cu&lt;2+&gt; as well as preparation method and application of fluorescent probe

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specific Embodiment approach 1

[0037] Specific embodiment one: the detection of Cu in this embodiment 2+ and can utilize Cu 2+ Detection of HPO 4 2– The fluorescent probe, its structural formula is:

[0038]

specific Embodiment approach 2

[0039] Specific embodiment two: the detection Cu of specific embodiment one 2+ and can utilize Cu 2+ Detection of HPO 4 2– The preparation method of the fluorescent probe is carried out according to the following steps:

[0040] The ratio of the amount of substance added to the alcohol reagent is 1: (1-5) of 2-(3-aminophenyl)-1-H-phenanthrene[9,10-d]imidazole and pyrrole-2-carbaldehyde, Then add acid as a catalyst, and stir at room temperature for 2 to 8 hours; add distilled water to the reaction system after the reaction, and solids are precipitated; filter the filter cake repeatedly with organic solvent I, collect the filter cake and dry the product as crude product. The crude product was recrystallized from organic solvent II to obtain the detected Cu 2+ and can utilize Cu 2+ Detection of HPO 4 2– fluorescent probes.

specific Embodiment approach 3

[0041] Embodiment 3: The difference between this embodiment and Embodiment 2 is that the alcohol solvent is methanol, ethanol, propanol, isopropanol or butanol; others are the same as Embodiment 2.

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Abstract

The invention discloses a fluorescence probe for detecting Cu<2+> and detecting HPO4<2-> by utilizing Cu<2+> as well as a preparation method and application of the fluorescence probe, and relates to the fluorescence probe for Cu<2+> as well as the preparation method and application of the fluorescence probe. The invention aims to solve the technical problems that the existing fluorescent probes for detecting HPO4<2-> are few and are interfered by H2PO4 <->. The structural formula of the fluorescent probe disclosed by the invention is shown in the specification. The fluorescent probe is obtained by reacting 2-(3-aminophenyl)-1-H-phenanthro [9, 10-d] imidazole with pyrrole-2-formaldehyde. The fluorescence intensity of the fluorescence probe solution and the fluorescence intensity of a mixture obtained after the fluorescence probe solution is added into a sample I to be tested by using a fluorescence spectrophotometer, and if the fluorescence intensity is reduced, it is judged that Cu<2+>exists in the sample I to be tested; and a sample II to be detected is continuously added into the Cu<2+>-containing solution, if the fluorescence intensity is enhanced, it is judged that HPO4<2-> exists in the sample II to be detected, and detecting HPO4<2-> with naked eyes. The method can be used in the field of Cu<2+> and HPO4<2-> detection, especially HPO4<2-> in feed.

Description

technical field [0001] The invention relates to a method for detecting Cu 2+ Fluorescent probes and their preparation methods and applications. Background technique [0002] Copper is a trace element that exists in the human body and in nature. It is the earliest metal used by humans and belongs to heavy metal ions. Excessive copper ions will not only cause environmental pollution, but also affect the normal growth of animals and plants. Phosphate at physiological pH as H 2 PO 4 – and HPO 4 2– It is the basic structural unit of biological systems and plays a key role in many biochemical processes. Hydrogen phosphate can be used as a phosphorus supplement in feed processing, providing phosphorus nutrition for livestock and poultry feed. Phosphorus substances can accelerate the growth and development of livestock and poultry; improve the mating rate and survival rate of livestock and poultry; at the same time enhance the disease resistance and cold resistance of livest...

Claims

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Application Information

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IPC IPC(8): C07D403/12C09K11/06G01N21/64
CPCC07D403/12C09K11/06G01N21/643C09K2211/1029C09K2211/1044G01N2021/6432
Inventor 阚伟杜佳慧李安然赵冰尹浩纯王丽艳孙立李硕
Owner QIQIHAR UNIVERSITY
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