Probiotic feed additive for improving immunity of aquatic animals
A feed additive, aquatic animal technology, applied in animal feed, animal feed, microorganism-based methods, etc., can solve the problems of poor product effect, slow spore germination, low germination rate, etc., to maintain intestinal flora and eliminate odors. , the effect of improving digestion and absorption rate
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Embodiment 1
[0041] A probiotic feed additive for improving the immunity of aquatic animals, the specific preparation process steps include:
[0042] (1) Preparation of fermentation broth: according to the mass percentage, 2% bran, 2% soybean meal, 1% peptone, 1% corn steep liquor, 0.02% potassium dihydrogen phosphate, 0.01% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, calcium chloride 0.01%, the balance ratio is the proportion of water to prepare a liquid fermentation broth, and adjust the pH to 6.5-7.0;
[0043] (2) Aerobic fermentation: Inoculate the seed liquid of Saccharomyces cerevisiae and Bacillus, the inoculum amounts are 2% and 3% respectively, the fermentation temperature is controlled at 28-37°C, and the rotation speed is 150-300rpm; ferment for 24 hours under these fermentation conditions; the spores Bacillus is a mixed bacterial agent of Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus, and its inoculation ratio is 1:2:1;
[0044] (3) Anaerobic ferm...
Embodiment 2
[0052] (1) Preparation of fermentation broth: according to the mass percentage, 2% bran, 2% soybean meal, 1% peptone, 1% corn steep liquor, 0.02% potassium dihydrogen phosphate, 0.01% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, calcium chloride 0.01%, the balance ratio is the proportion of water to prepare a liquid fermentation broth, and adjust the pH to 6.5-7.0;
[0053] (2) Aerobic fermentation: Inoculate the seed liquid of Saccharomyces cerevisiae and Bacillus, the inoculum amounts are 2% and 3% respectively, the fermentation temperature is controlled at 28-37°C, and the rotation speed is 150-300rpm; ferment for 24 hours under these fermentation conditions; the spores Bacillus is a mixed bacterial agent of Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus, and its inoculation ratio is 1:2:1;
[0054] (3) Anaerobic fermentation: After the aerobic fermentation, inoculate Lactobacillus powder, the inoculation amount is 3%, the fermentation temperatur...
Embodiment 3
[0062] A probiotic feed additive for improving the immunity of aquatic animals, the specific preparation process steps include:
[0063] (1) Preparation of fermentation broth: according to the mass percentage, 2% bran, 2% soybean meal, 1% peptone, 1% corn steep liquor, 0.02% potassium dihydrogen phosphate, 0.01% dipotassium hydrogen phosphate, 0.02% magnesium sulfate, calcium chloride 0.01%, the balance ratio is the proportion of water to prepare a liquid fermentation broth, and adjust the pH to 6.5-7.0;
[0064] (2) Aerobic fermentation: Inoculate the seed liquid of Saccharomyces cerevisiae and Bacillus, the inoculum amounts are 2% and 3% respectively, the fermentation temperature is controlled at 28-37°C, and the rotation speed is 150-300rpm; ferment for 24 hours under these fermentation conditions; the spores Bacillus is a mixed bacterial agent of Bacillus subtilis, Bacillus licheniformis and Bacillus pumilus, and its inoculation ratio is 1:2:1;
[0065] (3) Anaerobic ferm...
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