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Culture medium capable of improving chitinase activity of hirsutella sinensis mycelia

A technology of Chitinase and Chitinase in China, which is applied in the direction of enzymes, hydrolytic enzymes, fungi, etc., can solve the problems of low infection rate of bat moth larvae and difficulties in artificial cultivation of Cordyceps sinensis, and improve the activity of chitinase Effect

Pending Publication Date: 2020-09-22
YUNNAN UNIV
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Problems solved by technology

[0005] The object of the present invention is to provide a kind of culture medium that can improve the chitinase activity of Mortierella chinensis mycelium, solve the low infection rate of Mortierella chinensis mycelium to bat moth larvae, thereby causing difficulties in the artificial cultivation of Cordyceps sinensis The problem

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  • Culture medium capable of improving chitinase activity of hirsutella sinensis mycelia
  • Culture medium capable of improving chitinase activity of hirsutella sinensis mycelia

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[0026] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0027] 1. Preparation of chitin suspension

[0028] Take chitin in small pieces, grind it into powder with a juicer, weigh 60.0g of powdered chitin, slowly add 150mL of 36.6% hydrochloric acid, stir vigorously on a magnetic stirrer for 10min, and then transfer to 37 Keep the water bath at ℃ for 1 hour, shake and mix 2-3 times during this period, then stir vigorously with a magnetic stirrer for 10 minutes, move to a water bath at 37℃ and stand still for 3 hours, shake and mix 5-8 times during this period, until the solution is dark brown and free of chitin After powdering the residue, neutralize to...

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Abstract

The invention discloses a culture medium capable of improving chitinase activity of hirsutella sinensis mycelia. The culture medium comprises chitin. Chitinase plays a key role in degrading chitin when entomogenous fungi invade insect epidermis, and the enzyme activity of chitinase directly influences the efficacy of the entomogenous fungi infecting the insect epidermis. How to improve the enzymeactivity of hirsutella sinensis chitinase is a key step of ophiocordyceps sinensis formation. By improving the enzyme activity of hirsutella sinensis mycelia, the infection capacity of the hirsutellasinensis mycelia to hepialus armoricanus larvae can be effectively improved, and therefore a theoretical basis is laid for artificial cultivation of ophiocordyceps sinensis.

Description

technical field [0001] The invention relates to microorganism culture technology, in particular to a culture medium capable of improving the chitinase activity of Mortierella sinensis mycelia. Background technique [0002] Cordyceps ( Ophiocordyceps sinensis ), belonging to the kingdom Fungi ( Fungi ), Ascomycota ( Ascomycota ), Ascomycetes ( Pezizomycotina ), Hypocreales ( Sordariomycetes ), Nematodeceae ( Ophiocordycipitaceae ), Nematodes ( Ophiocordyceps ), which is a complex of insects and bacteria formed after the larvae of Lepidoptera bat moths are infected by Cordyceps sinensis anamorphic trichospores. Cordyceps sinensis is mainly distributed in Tibet, Gansu, Qinghai, Yunnan, Sichuan and other alpine meadows at an altitude of 3000m-5000m. It is a unique insect-growing fungus in the Qinghai-Tibet Plateau. Its main active ingredients include Cordyceps polysaccharides, cordycepic acid, polypeptides, various amino acids, fatty acids, nucleosides, ergosterol, ...

Claims

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Application Information

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IPC IPC(8): C12N1/38C12N1/14C12N9/42C12R1/645
CPCC12N1/38C12N1/14C12N9/2442C12Y302/01014
Inventor 沙涛何志红张亚平乐瑞
Owner YUNNAN UNIV
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