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Bionic composite bone scaffold and preparation method thereof

A bone scaffold and composite solution technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve problems such as poor mechanical properties, difficulty in meeting the requirements for replacement of hard tissues such as bones and teeth, and large damage to macromolecular structures.

Active Publication Date: 2020-09-18
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, the above-mentioned regenerated silk (silk fibroin, sericin) protein material also has poor mechanical properties, and it is difficult to meet the requirements of hard tissue replacement such as bones and teeth. The macromolecular structure of protein and sericin is greatly damaged, and the degree of polymerization (molecular weight) of the macromolecule is an extremely important internal factor determining the performance of the macromolecule

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0026] Aiming at the problems in the prior art that the silk fibroin macromolecules are severely damaged or the silk fibroin sericin composite solution is prepared separately, which affects the strength and modulus of the silk fibroin sericin composite skeleton, this application provides a bionic composite skeleton, which The composite skeleton is prepared from a silk fibroin sericin composite solution. During the preparation process of the silk fibroin sericin biomimetic composite skeleton, a large molecular weight and high viscosity silk fibroin sericin protein composite solution was prepared by using deionized water temperature, degumming, and one-step dissolution method. The method can avoid the destruction of silk fiber macromolecules, and finally make the silk fibroin in the prepared silk fibroin sericin composite solution have higher viscosity and large molecular weight, and then the silk fibroin sericin biomimetic composite skeleton prepared therefrom has Good compressi...

Embodiment 1

[0041] 1. Using boiled cocoon and reeled silkworm raw silk as raw material, take 1 kg of silkworm raw silk, take 50 L of deionized silk at a bath ratio of 1:50 (g / mL), preheat it to 60°C, put it into the raw silk and stir it at a constant temperature of 60°C 1 to 10 minutes, then take it out and wash the silk with deionization at 25 to 30°C, dehydrate it, and dry it with hot air at 40 to 60°C;

[0042] 2. Put the dried silk in 90°C preheated deionized water according to the bath ratio of 1:50 (g / mL) and cook at a controlled constant temperature for 3 hours, then take out the silk containing sericin and wash it thoroughly with deionized water at 25-30°C , after dehydration, dry with hot air at 40-60°C;

[0043] 3. The measured degumming rate of silk is 14%;

[0044] 4. Weigh the dried silk and dissolve it in a 9.3M lithium bromide aqueous solution at a bath ratio of 1:25 (g / mL), and treat it at 65°C until the silk is completely dissolved to obtain a silkworm silk fibroin seric...

Embodiment 2

[0049] 1. Using boiled cocoon and reeled silkworm raw silk as raw material, take 1 kg of silkworm raw silk, take 50 L of deionized silk at a bath ratio of 1:50 (g / mL), preheat it to 60°C, put it into the raw silk and stir it at a constant temperature of 60°C 1 to 10 minutes, then take it out and wash the silk with deionization at 25 to 30°C, dehydrate it, and dry it with hot air at 40 to 60°C;

[0050] 2. The dried silk is placed in 90°C preheated deionized water and boiled at a controlled temperature for 6 hours according to the bath ratio of 1:50 (g / mL), and the silk containing sericin is taken out and fully cleaned with deionized water at 25-30°C , after dehydration, dry with hot air at 40-60°C;

[0051] 3. The measured degumming rate of silk is 21.8%;

[0052] 4. Weigh the dried silk and dissolve it in a 9.3M lithium bromide aqueous solution at a bath ratio of 1:25 (g / mL), and treat it at 65°C until the silk is completely dissolved to obtain a silkworm silk fibroin serici...

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Abstract

The invention provides a preparation method for a bionic composite bone scaffold. The preparation method comprises the following steps: A) placing silk into warm water for pretreatment, then placing the silk into water with a temperature of 65-95 DEG C for treatment, and carrying out drying so as to obtain primarily treated silk; B) treating the primarily treated silk into a neutral salt solution,and re-purifying an obtained silk fibroin and sericin composite dissolved solution so as to obtain a silk fibroin and sericin composite solution; and C) concentrating the silk fibroin and sericin composite solution, carrying out freeze-drying, placing an obtained initial skeleton into micromolecular monohydric alcohol for treatment, and carrying out freeze-drying so as to obtain the bionic composite bone scaffold. According to the invention, the silk fibroin and sericin composite solution is prepared by adopting a mild degumming and one-step dissolving method, and the bionic composite bone scaffold prepared by utilizing the silk fibroin and sericin composite solution has high strength, high modulus and high toughness; and the bionic composite bone scaffold solves the neck-clamping technical problem of insufficient mechanical properties of a natural biopolymer tissue engineering scaffold for bone regeneration and repair.

Description

technical field [0001] The invention relates to the technical field of biomedical materials, in particular to a bionic composite bone scaffold and a preparation method thereof. Background technique [0002] Bone or teeth are the hardest connective tissue in the human body. Taking bone as an example, with the aging of the world population and the increase in accidents, there are countless patients with bone diseases, and the clinical medical treatment related to bones is facing more and more problems. pressure, especially bone repair and bone grafting. As early as the 19th century, bone graft surgery was used to repair large-scale bone defects. At present, common bone repair methods include traditional autologous bone grafting, allogeneic bone grafting, and artificial bone grafting. Although autologous bone graft repair has the best therapeutic effect, there are many factors such as damage to the donor site, insufficient bone graft, and complications. Allograft bone transp...

Claims

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Application Information

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IPC IPC(8): A61L27/48A61L27/50
CPCA61L27/48A61L27/50A61L2430/02C08L89/00
Inventor 王建南裔洪根殷音
Owner SUZHOU UNIV
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