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Bovine sperm cryoprotectant and preparation method thereof

A technology for cryopreservation and sperm, applied in the field of bovine sperm cryopreservation and its preparation, can solve the problems of stability, sperm oxidative stress, etc., and achieve the effects of enhanced stability, improved quality and antioxidant capacity

Inactive Publication Date: 2020-09-18
太东(镇江)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to overcome the defects of the prior art. The present invention proposes a bovine sperm cryopreservation solution and its preparation method, which solves the stability problem caused by the addition of egg yolk substances in the existing products, and the cryopreservation Oxidative Stress Response of Sperm During

Method used

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  • Bovine sperm cryoprotectant and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of liquid A:

[0032] Weigh 35g Tris, 17g citric acid and 12.5g fructose, dissolve the above components in 500mL water for injection; weigh 30g modified lecithin, dissolve it in 300mL 55°C water for injection and mix with the above mixture; Add 0.2g sesamol, 1g lycopene, 0.04g baicalein, 20g β-lactoglobulin and 1000000IU gentamicin to the solution; dilute the above solution to 1L, adjust the pH of the solution to 6.8 with HCl or NaOH After filtering through a 0.22μm filter membrane, the prepared solution was aseptically dispensed into 100mL bottles and stored at 2-8°C in the dark.

[0033] Preparation of liquid B:

[0034] Weigh 35g Tris, 17g citric acid and 12.5g fructose, dissolve the above components in 500mL water for injection; weigh 30g modified lecithin, dissolve it in 300mL 55°C water for injection and mix with the above mixture; Add 120mL glycerin and 1000000IU gentamycin to the solution; dilute the above solution to 1L, adjust the pH of the solut...

Embodiment 2

[0036] Preparation of liquid A:

[0037] Weigh 34.5g Tris, 16.5g citric acid and 12g fructose, and dissolve the above components in 500mL water for injection; weigh 29.5g modified lecithin, dissolve it in 300mL 55°C water for injection and mix it with the above mixture; Add 0.15g of sesamol, 0.9g of lycopene, 0.03g of baicalein, 19.5g of β-lactoglobulin and 1000000IU of gentamycin to the above mixed solution; dilute the above solution to 1L, adjust the pH of the solution with HCl or NaOH After adjusting to 6.8, filter through a 0.22 μm filter membrane, then aseptically dispense the prepared solution into 100 mL bottles, and store in the dark at 2-8°C.

[0038] Preparation of liquid B:

[0039] Weigh 34.5g Tris, 16.5g citric acid and 12g fructose, dissolve the above components in 500mL water for injection; weigh 32.5g modified lecithin, dissolve it in 300mL 55°C water for injection and mix it with the above mixture; Add 120mL glycerin and 1000000IU gentamicin to the above mix...

Embodiment 3

[0041] Weigh 35.5g Tris, 17.5g citric acid and 13g fructose, and dissolve the above components in 500mL water for injection; weigh 30.5g modified lecithin, dissolve it in 300mL 55°C water for injection and mix it with the above mixture; Add 0.25g of sesamol, 1.1g of lycopene, 0.05g of baicalin, 20.5g of β-lactoglobulin, and 1,000,000 IU of gentamycin to the above mixed solution; dilute the above solution to 1L, and adjust the pH of the solution with HCl or NaOH After adjusting to 6.8, filter through a 0.22 μm filter membrane, then aseptically dispense the prepared solution into 100 mL bottles, and store in the dark at 2-8°C.

[0042] Preparation of liquid B:

[0043] Weigh 35.5g Tris, 17.5g citric acid and 13g fructose, and dissolve the above components in 500mL water for injection; weigh 33.5g modified lecithin, dissolve it in 300mL 55°C water for injection and mix it with the above mixture; Add 120mL glycerin and 1000000IU gentamicin to the above mixed solution; dilute the ...

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Abstract

The invention provides a bovine sperm cryoprotectant. The cryoprotectant comprises a solution A and a solution B. The solution A comprises the following components by mass: 35.0 + / - 0.5 g of Tris, 17.0 + / - 0.5 g of citric acid, 12.5 + / - 0.5 g of fructose, 30 + / - 0.5 g of modified lecithin, and 1000000 IU of gentamicin, and is dissolved in water for injection. The solution B comprises the followingcomponents by mass: 35.0 + / - 0.5 g of Tris, 17.0 + / - 0.5 g of citric acid, 12.5 + / - 0.5 g of fructose, 30 + / - 0.5 g of modified lecithin, 12% of glycerol and 1000000 IU of gentamicin, and is dissolved by water for injection. Egg yolk is replaced with modified lecithin, so that the stability of the product is enhanced, and by adding sesamol, lycopene, baicalein and beta-lactoglobulin, the qualityand oxidation resistance of unfrozen sperms are improved.

Description

technical field [0001] The invention relates to a bovine sperm cryopreservation solution and a preparation method thereof. Background technique [0002] Semen cryopreservation technology is an advanced animal reproduction technology. Its basic principle is to prolong the survival time of sperm in vitro by reducing or inhibiting the metabolic intensity of sperm. With the limitation of the life of the breeding stock, the utilization rate of the good male stock is greatly improved, and the economic benefit is improved. [0003] At present, most of the existing sperm cryopreservation solutions on the market have added egg yolk, but the egg yolk comes from eggs, and the content of ingredients in different batches of eggs is not the same, which makes the product quality unstable, and the biologically active protein substances in the egg yolk may cause Potential allergic reaction, difficult to remove in post-cleaning. [0004] In the process of step-by-step cryopreservation and r...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226
Inventor 刘年
Owner 太东(镇江)生物科技有限公司
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