Oyster peptide and cordyceps mycelium composition with immunity enhancing function and application thereof
A technology of Cordyceps mycelium and immunity enhancement, applied in the field of food processing, can solve the problem of restricting the development and utilization of functional food of oyster peptide and fermented Cordyceps mycelium, limiting the high-value development and utilization of Cordyceps mycelium and economic benefits, pure bacteria The problem of single function of powder products is to achieve excellent immune enhancement function, excellent sensory flavor and easy development.
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Embodiment 1
[0031] Example 1: Oyster Peptide Cordyceps Mycelia Composition and Product Development
[0032] The parts by weight of the components in the oyster peptide Cordyceps mycelium composition with the function of enhancing immunity provided by the present invention are: 25-48 parts of oyster peptide, 30-45 parts of Cordyceps mycelium, 5 parts of Astragalus extract servings - 20 servings, wolfberry extract 2 servings - 10 servings.
[0033] Oyster peptide is a small molecular oligopeptide with a molecular weight of less than 1000Da and a protein content greater than 75%. The preparation method of oyster peptide is as follows:
[0034] (1) Add 200kg of water to 100kg of fresh oysters, soak for 1 hour, and then drain the water; repeat soaking in water twice to obtain desalted oysters; shell the desalted oysters, remove the viscera to obtain desalted oyster meat, and use a tissue masher Homogenizing desalted oyster meat to obtain oyster slurry.
[0035] (2) Add 20L water to 10kg oyst...
Embodiment 2
[0048] Example 2: Oyster Peptide Mycelia Composition Enhances Immunity Function Animal Experiment
[0049] 1 Experimental materials and methods
[0050] 1.1 Experimental materials
[0051] Oyster peptide, oyster peptide Cordyceps mycelium composition sample 1;
[0052] Positive control drug: levamisole hydrochloride.
[0053] 1.2 Experimental animals and grouping
[0054] 135 female BALB / C mice (SPF grade), 6-8 weeks old, weighing 18-22 g, were randomly divided into 9 groups, 15 in each group.
[0055] 1.3 Experimental method
[0056] After 3 days of adaptive feeding, 135 healthy female BALB / C mice were randomly divided into 9 groups: blank control group (NC), model control group (MC), positive control group (PC), oyster peptide low-dose group ( S1-L), oyster peptide medium dose group (S1-M), oyster peptide high dose group (S1-H), oyster peptide Cordyceps mycelium composition low dose group (S2-L), oyster peptide Cordyceps mycelium Composition medium dose group (S2-M), o...
Embodiment 3
[0084] Example 3: Oyster peptide mycelium composition enhances immune function cell experiment
[0085] 1 Experimental materials and methods
[0086] 1.1 Experimental materials
[0087] Oyster peptide solution, oyster peptide Cordyceps mycelium composition solution, macrophage RAW264.7, cultured with Gibco Eagle supplemented with 10% fetal bovine serum (FBS) at 37°C, 5% CO 2 Cultured in an incubator, the cells were cultured for 36-48 hours to reach the logarithmic growth phase, and used for subsequent experiments.
[0088] 1.2 Experimental grouping:
[0089]The macrophages RAW264.7 were divided into blank control group (NC), positive control group (PC), low-dose oyster peptide group (S1-L), middle-dose oyster peptide group (S1-M), high-dose oyster peptide group (S1-H), oyster peptide Cordyceps mycelium composition low dose group (S2-L), oyster peptide Cordyceps mycelium composition medium dose group (S2-M), oyster peptide Cordyceps mycelium high dose group (S2 -H).
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