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Method for extracting fish scale polysaccharide by adopting dynamic high-pressure microjet pretreatment

A high-pressure micro-jet, pre-treatment technology, applied in the direction of antitoxins, drug combinations, etc., to achieve the effect of improving the extraction yield and in vitro antioxidant capacity

Inactive Publication Date: 2020-09-04
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the application of dynamic high-pressure microfluidic technology to the extraction of polysaccharides from fish scales has not been reported.

Method used

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  • Method for extracting fish scale polysaccharide by adopting dynamic high-pressure microjet pretreatment
  • Method for extracting fish scale polysaccharide by adopting dynamic high-pressure microjet pretreatment
  • Method for extracting fish scale polysaccharide by adopting dynamic high-pressure microjet pretreatment

Examples

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Effect test

Embodiment 1

[0047] A method for extracting polysaccharides from fish scales using dynamic high-pressure micro-fluidic pretreatment, comprising the following steps:

[0048] (1) Clean the fresh carp scales, decalcify them, add the scales into the phosphoric acid solution, the mass volume ratio of the scales to the phosphoric acid solution is 1g: 30mL, the concentration of the phosphoric acid solution is 0.6mol / L, at a speed of 60r / min Stir at lower temperature for 40 minutes, filter through a 100-mesh filter cloth to obtain decalcified fish scales, then dry at 48°C for 2 hours, and pulverize until the particle size is 80 mesh to obtain fish scale powder, which is set aside;

[0049] (2) Mix the fish scale powder obtained in step (1) with water, the mass volume ratio of fish scale powder and water is 1g:20ml, stir evenly, the stirring speed is 200r / min, and the stirring time is 1min, and then use a dynamic high-pressure nano homogenizer Treat 2 times, each treatment pressure is 50MPa, treat...

Embodiment 2

[0052] A method for extracting polysaccharides from fish scales using dynamic high-pressure micro-fluidic pretreatment, comprising the following steps:

[0053] (1) Fresh grass carp fish scales are washed, decalcified, fish scales are added in phosphoric acid solution, the mass volume ratio of fish scales and phosphoric acid solution is 1g: 40mL, the concentration of described phosphoric acid solution is 0.8mol / L, at 80r / min Stir for 50 minutes at a rotating speed of 150 mesh, filter through a 150-mesh filter cloth to obtain decalcified fish scales, then dry at a temperature of 50°C for 2.2 hours, and pulverize until the particle size is 100 mesh to obtain fish scale powder. spare;

[0054] (2) Mix the fish scale powder obtained in step (1) with water, the mass volume ratio of fish scale powder and water is 1g: 30ml, stir evenly, the stirring speed is 250r / min, the stirring time is 1.5min, and then use dynamic high pressure nano homogenizer The instrument was treated 3 times,...

Embodiment 3

[0057] A method for extracting polysaccharides from fish scales using dynamic high-pressure micro-fluidic pretreatment, comprising the following steps:

[0058] (1) Fresh carp fish scales are washed, decalcified, and fish scales are added in the phosphoric acid solution, the mass volume ratio of fish scales and phosphoric acid solution is 1g: 50mL, the concentration of described phosphoric acid solution is 1.2mol / L, at 100r / min Stir for 60 minutes at a rotating speed of 200 mesh, filter through a 200-mesh filter cloth to obtain decalcified fish scales, then dry at a temperature of 53°C for 2.5 hours, and pulverize until the particle size is 120 mesh to obtain fish scale powder. spare;

[0059] (2) Mix the fish scale powder obtained in step (1) with water, the mass volume ratio of fish scale powder and water is 1g:40ml, stir evenly, the stirring speed is 300r / min, and the stirring time is 2min, and then use a dynamic high-pressure nano homogenizer Treat 4 times, each treatment...

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Abstract

The invention discloses a method for extracting fish scale polysaccharide by adopting dynamic high-pressure microjet pretreatment. The method comprises the following steps: cleaning fresh fish scales,and sequentially decalcifying, drying and crushing to obtain fish scale powder; uniformly mixing the fish scale powder with water, and treating by using a dynamic high-pressure nano homogenizer to obtain a mixture of the fish scale powder and the water; and carrying out water bath extraction on the obtained mixture of the fish scale powder and water, centrifuging, and collecting supernatant to obtain a product containing fish scale polysaccharide. The average particle size of the fish scale powder can be effectively reduced through dynamic high-pressure micro-jet pretreatment; the extractionyield of the fish scale polysaccharide is improved; along with the increase of processing pressure, the removal rate of the polysaccharide extracting solution on DPPH.,. O2., ABTS. + and. OH and the reduction capacity of Fe < 3 + > are gradually enhanced and are significantly higher than the blank control group. According to the method for extracting the fish scale polysaccharide by adopting the dynamic high-pressure micro-jet pretreatment, the extraction yield and the in-vitro oxidation resistance of the fish scale polysaccharide can be effectively improved.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide extraction, and more specifically relates to a method for extracting polysaccharides from fish scales through dynamic high-pressure micro-jet pretreatment. Background technique [0002] Carp is widely distributed in Eurasia and is one of the main aquaculture species in the world. my country is the largest carp breeding country with an annual output of 3.02 million tons, accounting for 80% of the world's total output. Every 100g carp meat contains 17.6g of protein, 4.1g of fat, 204mg of phosphorus, 50mg of calcium and multivitamins; moreover, the fat in carp is mostly unsaturated fatty acid, which has the functions of lowering human cholesterol, preventing arteriosclerosis and coronary heart disease, etc. Carp is considered a healthy food. [0003] Carp processing produces about 30% by-products, of which fish scales account for about 5%, and fish scales are usually treated as waste, causing...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P39/06
CPCC08B37/0003A61P39/06
Inventor 赵岩岩张浩崔震昆王书彦莫海珍赵圣明梁新红周威
Owner HENAN INST OF SCI & TECH
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