Complex enzyme preparation for extracting maize yellow pigment and extraction method

A technology of zeaxanthin and compound enzyme preparation, which is applied in the direction of chemical instruments and methods, biochemical equipment and methods, enzymes, etc., can solve the problems of low purity, complicated process of zeaxanthin, and low extraction rate, and achieve high purity, Increased freeness and easy operation

Inactive Publication Date: 2020-08-25
WUHAN SUNHY BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the complex process of extracting zeaxanthin in the existing corn gluten powder, and the problems of low extraction rate and low purity

Method used

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  • Complex enzyme preparation for extracting maize yellow pigment and extraction method
  • Complex enzyme preparation for extracting maize yellow pigment and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] According to mass percentage, cellulase 5%, β-mannanase 5%, xylanase 8%, glucosidase 2.5%, protease 15%, lipase 2%, α-amylase 10%, glucose 5% amylase and 1% pectinase, weigh the required quality biological enzyme preparation, make up 100% with water, and mix evenly to obtain the compound biological enzyme preparation.

[0023] Crush corn gluten powder to 80 mesh, add water to soak according to the ratio of material to liquid 1:6, adjust the pH value to 4.5 with dilute hydrochloric acid, add 5% of the above-mentioned compound biological enzyme preparation, keep warm at 45°C for enzymolysis reaction for 1 hour, and then centrifuge After removing the supernatant, add 75% ethanol solution with a volume ratio of 1:5 to the precipitate, ultrasonically extract for 30 minutes, and concentrate under low-temperature vacuum distillation; then add absolute ethanol to the concentrated solution at a volume ratio of 1:5, After stirring and dissolving for 15 minutes, the supernatant wa...

Embodiment 2

[0025] According to mass percentage, cellulase 2.5%, β-mannanase 8%, xylanase 2%, glucosidase 2%, protease 10%, lipase 5%, α-amylase 15%, glucose 7% amylase and 2% pectinase, weigh the required quality of the biological enzyme preparation, make up 100% with water, and mix evenly to obtain the compound biological enzyme preparation.

[0026] Crush corn gluten powder to 80 mesh, add water to soak according to the ratio of material to liquid at 1:10, adjust the pH value to 5.0 with dilute hydrochloric acid, add 1% of the above-mentioned compound biological enzyme preparation, keep warm at 50°C for enzymolysis reaction for 2 hours, and then centrifuge After removing the supernatant, add 80% ethanol solution with a volume ratio of 1:6 to the precipitate, ultrasonically extract for 30 minutes, and concentrate under low-temperature vacuum distillation; then add absolute ethanol to the concentrated solution at a volume ratio of 1:6, After stirring and dissolving for 15 minutes, the su...

Embodiment 3

[0028] According to mass percentage, cellulase 4%, β-mannanase 6%, xylanase 5%, glucosidase 2%, protease 12%, lipase 5%, α-amylase 12%, glucose 5% amylase and 2.5% pectinase, weigh the required quality biological enzyme preparation, make up 100% with water, and mix evenly to obtain the compound biological enzyme preparation.

[0029] Crush the corn gluten powder to 60 mesh, add water to soak according to the ratio of material to liquid at 1:7, adjust the pH value to 6.5 with dilute hydrochloric acid, add 4% of the above-mentioned compound biological enzyme preparation, keep warm at 30°C for enzymolysis reaction for 4 hours, and then centrifuge After removing the supernatant, add 70% ethanol solution with a volume ratio of 1:7 to the precipitate, ultrasonically extract for 25 minutes, and concentrate under low-temperature vacuum distillation; then add absolute ethanol to the concentrated solution at a volume ratio of 1:5, After stirring and dissolving for 15 minutes, the supern...

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Abstract

The invention provides a complex enzyme preparation for extracting a maize yellow pigment and an extraction method. The complex enzyme preparation comprises the following components in percentage by mass: 1 to 5% of cellulase, 5 to 10% of beta-mannase, 2 to 8% of xylanase, 10 to 20% of protease, 2 to 8% of lipase, 10 to 20% of alpha-amylase, 5 to 10% of glucoamylase, 1 to 5% of pectinase, 1 to 5%of glucosaccharase and the balance of water. The complex enzyme preparation for extracting the maize yellow pigment and the extraction method are simple in process and convenient to operate; enzymolysis preprocessing is carried out on corn gluten meal by adopting a biological enzyme method, macromolecular substances such as polysaccharides, proteins, pectins and the like which are connected with the maize yellow pigment are degraded, simultaneously, network structures of the proteins are hydrolyzed so as to improve freeness of the maize yellow pigment and make the maize yellow pigment easier to extract, and moreover, after enzymolysis processing, the maize yellow pigment and zein are separated by adopting two ethanol solutions with different concentration so as to obtain the high-purity maize yellow pigment; and an extraction rate of the maize yellow pigment can be up to 7.39%.

Description

technical field [0001] The invention belongs to the technical field of natural pigment extraction, and in particular relates to a compound enzyme preparation and an extraction method for extracting maize yellow pigment. Background technique [0002] Zeaxanthin is a natural food coloring, which belongs to isoprenoid pigments, and is a mixture of carotenoids such as β-carotene, cryptoxanthin, lutein and zeaxanthin. It is mainly obtained from corn gluten powder by solvent method extract. [0003] Corn gluten meal is a by-product of corn starch wet milling, which is the product left after corn starch, germ, and outer skin are removed. Corn gluten powder contains a large amount of protein (about 50%-60%) and natural pigments, a small amount of starch (about 15-20%) and cellulose (about 0.5%-2.5%) and other impurities, of which the protein is mainly: corn Glamins (68%), gluten (22%), globulins (1.2%) and a small amount of albumin. Corn gluten meal is usually used as a feed raw ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/42C12N9/50C12N9/24C12N9/20C12N9/26C12N9/34C12N9/18C12N9/88C09B61/00
CPCC09B61/00C12N9/18C12N9/20C12N9/2408C12N9/2414C12N9/2428C12N9/2437C12N9/2445C12N9/248C12N9/2491C12N9/50C12N9/88C12Y301/01003C12Y301/01011C12Y302/01001C12Y302/01003C12Y302/01015C12Y302/0102C12Y302/01021C12Y302/01025C12Y402/02002
Inventor 刘营付大波詹志春周樱
Owner WUHAN SUNHY BIOLOGICAL
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