A kind of microbial composite bacterial agent and its application in the preparation of fermented feed
A technology of compound bacterial agent and fermented feed, which is applied in the fields of fermentation and biology, can solve the problems of food safety, feed corruption, and low return, and achieve the effect of rich nutrition, non-corruption safety, and high safety
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Embodiment 1-1
[0066] Embodiment 1-1: Screening, identification, cultivation and observation of Bifidobacterium longum (Bifidobacterium longum)
[0067] 1. Screening
[0068] Take 1g of feces samples from healthy adults in Wuxi area, dilute them with normal saline, spread them on mMRS solid medium, culture them in an anaerobic environment at 37°C for 72 hours, observe and record the colony morphology; pick colonies in mMRS Streak on the solid medium, carry out purification culture at 37°C in an anaerobic environment, and obtain a purified single colony; pick a single colony and streak on the mMRS solid medium, culture anaerobically at 37°C for 48 hours, and test the obtained colony Gram staining (Gram staining method refers to the textbook "Industrial Microbial Breeding" author: Zhuge Jian), record the colony morphology, and investigate the physiological and biochemical characteristics of the strain according to the textbook "Common Bacterial System Identification Manual" (Author: Dong Xiuzh...
Embodiment 1-2
[0083] Example 1-2: Effect of Bifidobacterium longum CCFM1109 and its fermentation supernatant on the germination rate of filamentous fungal spores
[0084] 1. Effect of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 on the germination rate of filamentous fungal spores (double-layer plate-growth inhibition method)
[0085] Pick the single bacterium colony of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 screened in Example 1 and streak it on the mMRS solid medium, cultivate it at 37° C. for 48 hours in an anaerobic environment to obtain a single bacterium colony; pick a single bacterium colony and inoculate it in the mMRS liquid In the culture medium, culture was carried out at 37°C for 48 hours under anaerobic environment, and this operation was repeated 3 times to obtain the bacterial liquid cultivated to the third generation.
[0086] Use an inoculation loop to inoculate the Penicillium extensa liquid in the ampoule tube on the PDA medium, and incubate ...
Embodiment 1-3
[0102] Example 1-3: Effect of Bifidobacterium longum (Bifidobacterium longum) CCFM1109 fermentation supernatant on the growth of filamentous fungal mycelium
[0103] Mix the mMRS liquid medium with the PDA medium at a volume ratio of 1:9, 1.5:8.5, 2:8, 2.5:7.5, 3:7 (mMRS liquid medium: PDA medium) to obtain mMRS liquid medium Concentration is respectively the control group mixed solution of 10,15,20,25,30% (v / v); The fermentation supernatant that embodiment 2 obtains is respectively 1:9,1.5:8.5,2:8,2.5: The volume ratio of 7.5, 3:7 (fermentation supernatant: PDA medium) was mixed with PDA medium to obtain experimental groups with fermentation supernatant concentrations of 10, 15, 20, 25, 30% (v / v) respectively Mixed solution; Pour the mixed solution of the control group and the experimental group into the plate respectively; add 10 μL of the Penicillium expanse spore suspension obtained in Example 2 dropwise to the center of the plate, and cultivate it at 28°C for 6 days. The...
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