Sulindac and naproxen immunoassay card with marker guiding signal amplification effect

Abstract

The invention relates to a sulindac and naproxen immunoassay card with a marker guiding signal amplification effect. The sulindac and naproxen immunoassay card comprises a lining plate, and a difunctional antigen release pad, a universal fluorescent probe release pad, a chromatographic membrane and a water absorption pad which are sequentially adhered to the lining plate and are partially overlapped with one another, wherein the bifunctional antigen release pad comprises a specific marker coupled on the basis of a detection antigen formed by coupling ovalbumin with sulindac and naproxen respectively; the universal fluorescent probe release pad comprises a universal fluorescent probe labeled with an anti-specific marker antibody; the chromatographic membrane is provided with a detection line A, a detection line B and a quality control line which are respectively used for fixing an anti-sulindac antibody, an anti-naproxen antibody and an anti-specific marker antibody. According to the invention, the production of all detection reagents can be solved by using the universal fluorescent probe, the cost is reduced, and the quality control reliability is improved; and the signal intensityand the stability of detection are improved, and the detection sensitivity and the quantitative precision are improved.

Description

technical field [0001] The invention relates to the field of food and drug safety detection, in particular to an immunological detection card for sulindac and naproxen with a marker-guided signal amplification effect. Background technique [0002] Both sulindac and naproxen are non-steroidal anti-inflammatory drugs with anti-inflammatory, analgesic and antipyretic effects. Because these two kinds of analgesic effects are quick and cheap, unscrupulous manufacturers illegally add sulindac and naproxen to health food (mainly health wine) for rheumatic diseases to enhance the curative effect and seek illegitimate benefits. These two non-steroidal anti-inflammatory drugs are prescription drugs. Improper use may cause damage to the digestive tract, liver and kidneys, and even lead to anaphylactic shock and acute renal failure in severe cases. The long-term use of health food containing sulindac and naproxen without the knowledge of the patient is very harmful. It is necessary to ...

AI Technical Summary

Problems solved by technology

However, this method has the following disadvantages: 1. To detect different target substances, it is necessary to use corresponding detection antibodies to connect quantum dot microspheres to prepare fluorescent probes, which requires a large workload, low yield, and unstable quality; The reaction to prepare fluorescent probes may affect the structure and immunoaffinity of antibodies; 3. Lack of signal amplification mechanism, the detection signal intensity is weak and the sensitivity is low

However, the immunogenicity of the "detection antigen carrier protein" is unstable, and slight changes in protein conformation will lead to a decrease in the affinity between the "universal antibody" and the "detection antigen", weaken the formation of "sandwich immune complexes", and affect the detection signal. produce

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