Method for treating inner surface of microchannel

A micro-channel, inner surface technology, applied in chemical instruments and methods, laboratory containers, instruments, etc., can solve the problems of wrong detection results, sample residues, transportation, etc., to reduce residues and improve accuracy.

Active Publication Date: 2022-05-24
TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Residual blood samples can coagulate and then fall off and remain on top of the separation medium, making it difficult to interpret agglutination results
[0005] When using microfluidic chips for other immunological assays, it is also difficult to ensure that all samples to be tested are delivered to a specific reaction channel or chamber due to similar sample carryover
For micro-reactions, slight differences in sample volume may lead to errors in the interpretation of test results

Method used

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  • Method for treating inner surface of microchannel
  • Method for treating inner surface of microchannel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The microfluidic chip used in this example is as figure 1 shown. It includes a sample inlet 1 , a plurality of liquid separation channels 2 , a quantitative zone 3 , a stop valve 4 , a reaction zone 5 , a detection zone 6 and a gas hole 7 . The injection port 1 is used for sample loading, with a diameter of 3 mm. The dispensing channel 2 is used to deliver the sample to its respective downstream chambers, with an inner diameter of 0.1 mm. The quantitative area 3 is used to control the amount of sample entering each reaction area 5, and the total volume is 3 μL. The stop valve 4 is used to prevent the sample from entering the reaction zone 5 before centrifugation or before reaching a certain centrifugation speed. The reaction zone 5 is the zone where the sample reacts with the detection reagent, and the separation zone 6 can be used to observe the reaction result. The air hole 7 is used to eliminate the gas pressure generated by the liquid entering the dosing zone 3 ...

Embodiment 2

[0041] Using the same microfluidic chip as in Example 1 and a similar paraffin oil treatment method, the effect of temperature on sample residue was studied. The test process is as follows:

[0042] 1) Take 30 chips, 3 chips in each group, add 5 μL of paraffin oil into the injection port, fill the liquid separation channel and quantitative area, and place them for 5 hours under 10 different temperature conditions;

[0043] 2) Place absorbent paper with a diameter of 3 mm on the sample inlet, and reverse centrifugation at 200g for 3 min to suck out excess paraffin oil, repeat three times, and replace the absorbent paper each time;

[0044] 3) Weigh the processed microfluidic chip, record the weight, add 3 mg of whole blood sample from the injection port, place 3mm diameter absorbent paper on the injection port after filling the quantitative area and liquid separation channel, and reverse centrifuge at 200g After 3 minutes, the sample was fully sucked out, and then the microflu...

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Abstract

This paper provides a method for processing the inner surface of the microchannel, including 1) paraffin oil is delivered to the microchannel through a sample inlet communicated with the microchannel, so that the paraffin oil and the microchannel surface contact, and 2) the paraffin oil was removed from the injection port by reverse centrifugation. The microfluidic chip processed by the method can greatly reduce the residue of samples in the chip, so as to improve the accuracy of experimental results.

Description

technical field [0001] This paper relates to a method for treating the inner surface of a microchannel, especially a method for treating the inner surface of a microfluidic chip with paraffin oil. Background technique [0002] In recent years, microfluidic chip technology has developed rapidly, and its applications in medicine, life sciences, environmental monitoring and other fields have been gradually expanded. Numerous chambers and microchannels can be integrated on a microfluidic chip of several square centimeters to achieve various functions such as injection, dilution, mixing, separation, reaction, and detection. Microfluidic chips have significant advantages such as miniaturization, integration, fast detection, and low consumption of samples and reagents. [0003] As one of the most routine clinical analysis methods, blood analysis plays a very important role in disease diagnosis and treatment. An important aspect of blood analysis is the determination of transfusio...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00G01N33/53G01N33/80
CPCB01L3/502707B01L3/502753G01N33/80G01N33/5304B01L2200/10B01L2300/12
Inventor 王秀柱王丽王伟权黄志刚王亚强
Owner TIANJIN DEXIANG BIOTECHNOLOGY CO LTD
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