Method for accurately preparing acetic acid standard curve solution and detecting protected amino acid acetic acid

Abstract

The invention discloses a method for accurately preparing an acetic acid standard curve solution and detecting protected amino acid acetic acid, and the method comprises the following steps: S1, preparing an acetic acid stock solution, precisely weighing a designed amount of anhydrous potassium acetate, and adding ultrapure water to a constant volume according to a detection concentration demand to obtain the acetic acid stock solution with a designed concentration; S2, preparing an acetic acid standard curve solution, adding ultrapure water to dilute the stock solution to obtain an acetic acid standard curve solution with a designed concentration, and adding trifluoroethanol or methanol with a designed amount during preparation. By improving the formula of the standard curve, the preparation process of the standard curve is simpler, more convenient and more accurate, the use of an organic solvent is avoided, the volatilization of the solvent is reduced, and the detection cost is reduced; meanwhile, the interference peak and the target peak are effectively separated by adopting ion chromatography for detection and designing reasonable gradient conditions, and finally, the target peak is obvious in performance and easy to judge and calculate, so the problems of misjudgment and misjudgment in the prior art are solved.

Description

technical field [0001] The invention relates to the technical field of acetic acid detection of protected amino acids, in particular to a method for accurately preparing a standard curve solution of acetic acid and a detection method of acetic acid of protected amino acids. Background technique [0002] With the development of peptide synthesis, the market demand for protected amino acids is increasing, and the quality requirements for protected amino acids are becoming more and more stringent. Through a lot of practice, the acetic acid impurity contained in the protected amino acid has a great negative impact on the quality control of the final product, so the customer is very strict in the control of the acetic acid in the protected amino acid, usually controlled below 200ppm, and even less than 50ppm , and how to precisely control the acetic acid content, first of all, an efficient detection method with high accuracy and high detection precision is needed to detect the ac...

AI Technical Summary

Problems solved by technology

[0007] The first object of the invention of the present invention is: when preparing the acetic acid standard curve solution in the prior art, the concentration that exists is unstable, inaccurate, and the problem that the standard curve error is large provides a kind of method for accurately preparing the acetic acid standard curve solution, To solve the problems existing in the existing technology

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