Squalene engineering strain, squalene synthetic plasmid, cell membrane space expansion plasmid and preparation methods

Abstract

The invention relates to a squalene engineering strain, a squalene synthetic plasmid, a cell membrane space expansion plasmid and preparation methods. By constructing an engineering strain of a squalene synthetic pathway, developing a microbial cell factory and constructing a squalene synthetic unit of a gene related to membrane area expansion, the biosynthesis and industrial production process ofsqualene is promoted. According to the squalene engineering strain, the squalene synthetic plasmid and the preparation methods thereof, a green synthetic method for synthesizing the squalene in escherichia coli can reduce dependence on natural resources and overdevelopment. Moreover, the method is relatively low in cost, can realize large-scale squalene production, and has huge economic benefits.

Description

technical field [0001] The invention relates to a squalene engineering strain, a squalene synthesis plasmid, a cell membrane space expansion plasmid and a preparation method, belonging to the field of biological genetic engineering. Background technique [0002] Squalene is an unsaturated triterpenoid compound formed by linking 6 isoprenes, and it is a key intermediate product in the human cholesterol metabolism pathway. Squalene widely exists in animals, plants or microorganisms. In animals, the squalene content in the liver of deep-sea sharks is the most abundant. In addition, the content of squalene in yak meat is also relatively rich. The distribution of squalene in plants is also very wide, such as olive oil, pumpkin seeds, etc. are rich in squalene. Squalene has important physiological activities such as strong oxygen-carrying capacity, anti-oxidation, anti-radiation, detoxification, inhibition of microbial growth, regulation of cholesterol metabolism in animals, and...

AI Technical Summary

Problems solved by technology

[0004] However, the existing squalene production methods mainly include plant extraction, chemical synthesis and biosynthesis, etc. Plant extraction is limited by the plant cycle and the yield is low; chemical synthesis requires harsh conditions and the production process is low in safety. The cost of industrial synthesis is high; while the method of biosynthesis uses cheap culture medium and microorganisms that are easy to edit and have a short growth cycle. Compared with the harsh reaction conditions and high cost of chemical synthesis, it is cheaper and more efficient.

[0005] At present, foreign scholars have done some pioneering work on the biosynthesis of squalene, but because these methods are not ideal in terms of the yield of strains and target objects, and there are certain limitations, large-scale production cannot be carried out.

Images