Pseudomonas aeruginosa and screening method and application thereof

Abstract

The invention relates to pseudomonas aeruginosa and a screening method and application thereof. The strain number of the pseudomonas aeruginosa is LYT-4, and the pseudomonas aeruginosa is preserved inthe China General Microbiological Culture Collection Center on March 26, 2018, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No. 15519;and the screening method comprises the following steps: (1) adding water into naturally stacked and fermented plant meal, performing uniform stirring, and standing; (2) performing gradient dilution, coating inoculation and constant-temperature culture; and (3) picking out grown bacterial colonies, repeatedly performing streak inoculation, screening strains with high content of amino nitrogen, andidentifying 16SrDNA as pseudomonas aeruginosa. The pseudomonas aeruginosa is applied to plant meal protein fermentation or antagonism of plant pathogenic fungi. The method is convenient and rapid, andthe screened strain is high in purity. The application is simple and convenient to operate, low in cost and suitable for agricultural production.

Description

technical field [0001] The invention specifically relates to a strain of Pseudomonas aeruginosa and its screening method and application. Background technique [0002] Cake is the plant residue left after extracting vegetable oil. Although its protein content is high, it is mostly discarded because it is difficult to use. For example, tung cake (tung meal) is the main by-product left after tung oil is extracted from tung oil. Its protein content can reach 36-45%, and the amino acid content and essential amino acid content extracted from it can reach 93.8% and 45.4% respectively. , is an excellent plant protein resource available for development and utilization. In addition to the high content of protein, tung meal also contains 1-3% phosphorus and 1-2% potassium and other trace elements needed by plants. It is an ideal plant fertilizer substrate that can be developed and utilized. Although my country's tung oil output ranks first in the world, and the annual output of tung...

AI Technical Summary

Problems solved by technology

Although my country's tung oil output ranks first in the world, and the annual output of tung meal reaches 500,000 tons, the current utilization rate of tung meal resources is less than 20%, and most of them are discarded. pollution source

[0004] CN109439561A discloses a strain of Pseudomonas aeruginosa and its application. The Pseudomonas aeruginosa shows signs of infection including cabbage black spot fungus, sugarcane pineapple fungus, kumquat fungus, rice flax leaf spot fungus and linseed blight fungus. showed strong antagonistic properties, however, and did not have a strong antagonistic effect on the flax anthracnose bacteria ( Colletotrichum lini ) and Fusarium oxysporum ( Fusarium oxysporum ) and other antagonistic properties, and there is no research on the application of the bacteria to the fermentation of plant meal

[0005] CN110616179 A discloses a strain of Pseudomonas aeruginosa DGNK-JL2 and its application. The Pseudomonas aeruginosa has shown a better phosphorus-solubilizing effect, but its ability to ferment plant meal has not been studied; the aeruginosa Pseudomonas has a certain antagonistic effect on the pathogenic fungi pepper anthracnose and bitter gourd wilt, but it does not mention the bacteriostatic rate, and has no effect on flax anthracnose ( Colletotrichum lini ), flax anthracnose ( Colletotrichum lini ) and Fusarium oxysporum ( Fusarium oxysporum ) for antagonistic performance studies

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