A method for measuring the heart rate of zebrafish by using the difference of fluorescence signal

A fluorescent signal, zebrafish technology, applied in the fields of biological measurement and optics, can solve problems such as difficulty in improving measurement accuracy, achieve the advantages of measurement accuracy, reduce photobleaching, and display rich details.

Active Publication Date: 2022-07-29
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current existing methods are difficult to improve the measurement accuracy, the present invention improves the algorithm to obtain more details of the heartbeat signal

Method used

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  • A method for measuring the heart rate of zebrafish by using the difference of fluorescence signal
  • A method for measuring the heart rate of zebrafish by using the difference of fluorescence signal
  • A method for measuring the heart rate of zebrafish by using the difference of fluorescence signal

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Experimental program
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Effect test

Embodiment Construction

[0025] Measuring the effect of different concentrations of cardiac drugs on heart rate in zebrafish:

[0026] 1. Cultivate green fluorescent protein (GFP)-labeled heart zebrafish.

[0027] 2. Build as figure 1 As shown in the experimental system, connect the power supply to check whether it is working properly.

[0028] 3. Five groups of zebrafish were administered (the drug was bisphenol fluorene), and the first four groups were administered at concentrations of 1 μM, 2 μM, 5 μM, and 10 μM, respectively, and the fifth group was not administered.

[0029] 4. Anesthetize a zebrafish with a certain concentration and place it in a square capillary, and adjust the posture of the zebrafish. Use the appropriate magnification microscope objective to observe clear zebrafish hearts.

[0030] 5. Use high-speed CCD to collect images of heart beating.

[0031] 6. Use the written program to process the video to find the heart rate of the heart.

[0032] 7. The other four zebrafish at ...

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Abstract

A non-contact measurement of zebrafish heart rate that is less damaging to the organism, more stable and more precise. The GFP fluorescent protein in the genetically labeled zebrafish heart emits a fluorescent signal when illuminated by a laser corresponding to the excitation wavelength. The fluorescence signal (507 nm) was collected by using an inverted microscope equipped with a filter to filter out the excitation light signal (488 nm), and a high-speed CCD camera was used to collect heartbeat images over a period of time to form a video. The obtained video is extracted frame by frame and the area of ​​the difference area between frames is statistically drawn to obtain the variation curve of the difference over time. The heart beat cycle is obtained by counting the average of the average interval frames of peaks and the average interval of troughs, and the heartbeat frequency can be obtained by using the known video frame rate. This method has important application potential in biological measurement, biological in vivo microscopic imaging and so on.

Description

technical field [0001] The invention belongs to the technical fields of optics and biological measurement, and relates to the processing of micro-optical devices, the design of a microscopic imaging system and the imaging of living organisms. Its characteristic is to use the frame-by-frame method to extract the signal of the heart beat, which can restore the heart beat more completely. Background technique [0002] Zebrafish is widely used in biological experiments due to its rapid development and high transparency, and the measurement of its heart rate plays an important role in biological experiments and related drug research. At present, there are several methods to measure the heart rate of zebrafish. [0003] 1. Acupuncture method to measure the electrocardiogram of zebrafish: This method is to pierce two electrodes into the heart of zebrafish to detect electrical signals, and obtain the electrocardiogram of zebrafish after amplification. This method is more damaging ...

Claims

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Application Information

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IPC IPC(8): A61B5/024A61B5/00
CPCA61B5/02444A61B5/0071A61B2503/40
Inventor 匡登峰黄曾鑫
Owner NANKAI UNIV
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