Method for detecting influence of dietary fiber on activity of human intestinal bacteria

Abstract

The invention discloses a method for detecting influence of dietary fiber on activity of human intestinal bacteria in the field of bacterial activity detection. The method comprises the detection of bacteroides, and comprises the following specific steps: 1, preparing a bacteroides culture medium; 2, culturing the bacteroides until the bacteroides reaches a logarithmic phase, collecting the bacteroides, washing the bacteroides by using the bacteroides culture medium, and diluting bacteroides bacterial liquid by using the bacteroides culture medium until the OD600 value is 0.2 to 0.23; 3, in a96-pore plate, adding 100 microliters of diluted bacteroides bacterial liquid and 100 microliters of dietary fiber solution into each pore correspondingly, so that the bacterial concentration OD600 ineach pore is 0.09 to 0.11; culturing in a 37-DEG C constant-temperature anaerobic incubator for 72 hours, and recording the OD600 value every 12 hours; and 4, drawing a growth curve of the bacteroides under the action of the dietary fiber according to the OD600 value of the bacteroides recorded at each time point. According to the scheme, the bacteroides culture medium is optimized, so that the growth state of the bacteroides in the culture medium is more fit with the growth state of the bacteroides in a human intestinal tract, and the accuracy of a detection result is improved.

Description

technical field [0001] The invention belongs to the field of bacterial activity detection, in particular to a method for detecting the influence of dietary fiber on the activity of human intestinal bacteria. Background technique [0002] Gut flora refers to the microorganisms in the intestinal tract. There are about 10 trillion microorganisms in the human intestinal tract. These microorganisms play a very important role in regulating nutrient absorption, maintaining epithelial cell development, and regulating innate immunity. Firmicutes and Bacteroides occupied the largest number. [0003] Dietary fiber is an edible carbohydrate polymer containing three or more monomeric units that is resistant to endogenous digestive enzymes and is therefore neither hydrolyzed nor absorbed in the small intestine. Since dietary fiber cannot be digested and degraded by the human body itself, and the intestinal flora colonized in the human intestinal cavity is rich in polysaccharide hydrolyti...

AI Technical Summary

Problems solved by technology

[0005] The present invention intends to provide a method for detecting the influence of dietary fiber on the activity of human intestinal bacteria, so as to solve the problem in the prior art that the culture medium with unreasonable components is used to cultivate the microorganisms to be detected, thereby affecting the detection results

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