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Application of gene TaPT13 in improving resistance of plants to powdery mildew

A gene and plant technology, applied in botany equipment and methods, applications, plant peptides, etc., to achieve the effects of improving plant disease resistance, improving powdery mildew resistance, and promoting efficient absorption

Active Publication Date: 2020-07-17
HENAN INST OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Previous research on phosphorus transporters has mainly focused on the role of plants in the uptake and redistribution of inorganic phosphate, and the role of phosphorus transporters in plant defense has rarely been reported.

Method used

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  • Application of gene TaPT13 in improving resistance of plants to powdery mildew
  • Application of gene TaPT13 in improving resistance of plants to powdery mildew
  • Application of gene TaPT13 in improving resistance of plants to powdery mildew

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1.1 Mycorrhiza and low phosphorus treatment

[0025] The three-leaf stage wheat was transplanted into a culture medium with a 1:1:1 ratio of fine sand, vermiculite and perlite with two mycorrhizas of G. moses and G. terrestrial. The mycorrhizal inoculation amount was 200 Spores / 15g. Grow in a light incubator with a photoperiod of 16h / 8h and a temperature of 18℃ / 15℃. All inoculated and control plants are watered twice a week with 1 / 2 Hoagland nutrient solution, 5μM KH 2 PO 4 Low phosphorus treatment, 500μM KH 2 PO 4 After high-phosphorus treatment, take the wheat roots after 6 weeks, wash them with water, harvest root samples, and store them at -80°C after quick freezing in liquid nitrogen.

[0026] The mycorrhiza, low-phosphorus and high-phosphorus samples were used to extract total RNA using TRIzol Reagent (invitrogen).

[0027] Using 1 µg total RNA as a template, the first strand cDNA was synthesized using a cDNA synthesis kit, and the extracted RNA was reverse transcribed ...

Embodiment 2

[0034] Example 2 TRV virus-mediated functional analysis of TaPT13-VIGS plant

[0035] Using the full-length TaPT13 gene as a template, according to the characteristics of the TRV virus vector pYL156, the EcoR I and BamH I sites were selected to construct pYL156, and the recombinant vector pYL156-TaPT13 and empty pYL156 were transformed into Agrobacterium GV3101 (negative control) , Use the newly germinated wheat seeds to extract and transform TRV vector to realize the silence of the whole wheat.

[0036] Take the leaves of TaPT13-VIGS and control plants grown for 16 days and place them on a 1% agar plate supplemented with 85μM benzimidazole. Place the plates in a climate incubator at 20°C and incubate for 4 hours, and then use the shake-off method to obtain an appropriate density (About 250 conidia / cm 2 ) Gently shake the fresh conidia of powdery mildew to inoculate the leaves, and sample for 60h and 7d. After the leaves of TaPT13-VIGS and control plants were cultured in a climate...

Embodiment 3

[0048] Example 3 Functional analysis of overexpression of TaPT13 in Arabidopsis

[0049] In order to further analyze the function of TaPT13, the full length of TaPT13 was cloned and constructed into the plant vector CTAPi-GW-3HA which was started by 35S promoter, and the overexpression recombinant vector CTAPi-GW-3HA-TaPT13 was obtained. The recombinant vector and the empty vector were passed through The heat shock method was used to transform Agrobacterium GV3101, and the recombinant vector and empty vector were transformed into Colombian ecotype (Col-0) Arabidopsis thaliana by the method of dipping flowers. After screening, 7 single-copy homozygous transgenic lines were obtained. The roots of Arabidopsis and transgenic plants were simultaneously inoculated with Arabidopsis powdery mildew.

[0050] The results showed that, compared with the wild-type and the transgenic plants transformed with the empty vector, the seven transgenic lines overexpressing TaPT13 were significantly mor...

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Abstract

The invention belongs to the field of agricultural biology, and particularly relates to an application of a gene TaPT13 in improving resistance of plants to powdery mildew. The specific phosphorus transporter gene TaPT13 of arbuscular mycorrhiza can be used for improving disease resistance of plants, and the nucleotide sequence of the gene TaPT13 is shown in SEQ ID NO.1. According to the invention, the TaPT13 gene is determined to be a specific phosphate transporter gene of arbuscular mycorrhiza. After plants are inoculated with powdery mildew, the expression amount of the TaPT13 gene is obviously improved; and after the TaPT13 gene is silenced in the plants, susceptibility of the plants is increased, and the expression amount of a disease-resistant marker gene is reduced. Therefore, the TaPT13 gene can be used for improving resistance of wheat to powdery mildew, and has wide application space in plant breeding and cultivation.

Description

Technical field [0001] The invention belongs to the field of agricultural biology, and specifically relates to the application of the gene TaPT13 in improving the resistance of plants to powdery mildew. Background technique [0002] Phosphorus is the second largest element necessary for plant growth and development. It is widely involved in energy transfer, signal transduction, macromolecular biosynthesis, photosynthesis, and regulation of respiration and other metabolic processes, and plays an important role in plant growth. Phosphorus mainly exists in the soil in complex, insoluble and organic forms, while plants mainly obtain phosphorus in the form of inorganic phosphorus, so the phosphorus in the soil is difficult to absorb and utilize by plants. Plants have evolved a series of effective strategies to cope with the stress of low-phosphorus environment to promote the absorption and utilization of phosphorus, including changing root structure, root secretion of organic acids an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00A01H6/46C07K14/415C12N15/29
CPCC12N15/8282C07K14/415Y02A40/146
Inventor 李成伟胡海燕张怡卜瑞方魏琦超胡平
Owner HENAN INST OF SCI & TECH
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