Method for identifying apis cerana honey and apis mellifera honey
A technology of honey and honey bees, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems that do not conform to the development trend of efficient and rapid identification, restrict the healthy development of the Chinese bee industry, and reduce the content, so as to achieve health and safety The effects of sustainable development, promotion of industrialization development, and ease of operation and promotion
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0047] (1) Construction of discriminant model:
[0048] Weigh 3g of honey samples (respectively Chinese honey samples (see Table 1 for specific information) and Italian honey samples (see Table 1 for specific information)) and add 5ml of 10% methanol aqueous solution to dilute, ultrasonically dissolve and extract honey for 10 minutes, 12000rpm , centrifuged at 4°C for 10 min, and separated by liquid chromatography tandem high-resolution mass spectrometry. The liquid chromatography instrument conditions are: Thermo Scientific Hypersil GOLD C18 (100×2.1mm, 1.9μm) chromatographic column, column temperature 40°C, injection volume 5μL, 0.1% formic acid water as mobile phase A, acetonitrile as mobile phase B, Flow rate 0.3mL / min, using gradient elution program: 0-2min, 5% mobile phase B; 7min, 30% mobile phase B; 13-18min, 95% mobile phase B; 18.1-20min, 5% mobile phase B. The conditions of the high-resolution mass spectrometer are: spray voltage 3.5kV(+), 3.2kV(-); spray temperat...
Embodiment 2
[0052] (1) Construction of discriminant model:
[0053] Weigh 2g of honey samples (respectively Chinese honey samples (see Table 1 for specific information) and Italian honey samples (see Table 1 for specific information)) and add 8ml of 8% methanol aqueous solution to dilute, ultrasonically dissolve and extract honey for 10 minutes, 10000rpm , centrifuged at 4°C for 10 min, and separated by liquid chromatography tandem high-resolution mass spectrometry. The liquid chromatography instrument conditions are: Thermo Scientific Hypersil GOLD C18 (100×2.1mm, 1.9μm) chromatographic column, column temperature 40°C, injection volume 5μL, 0.1% formic acid water as mobile phase A, acetonitrile as mobile phase B, Flow rate 0.3mL / min, using gradient elution program: 0-2min, 5% mobile phase B; 7min, 30% mobile phase B; 13-18min, 95% mobile phase B; 18.1-20min, 5% mobile phase B. The conditions of the high-resolution mass spectrometer are: spray voltage 3.5kV(+), 3.2kV(-); spray temperatu...
Embodiment 3
[0057] (1) Model construction:
[0058] Weigh 5g of honey samples (respectively Chinese honey samples (see Table 1 for specific information) and Italian honey samples (see Table 1 for specific information)) and add 30ml of 12% methanol aqueous solution to dilute, ultrasonically dissolve and extract honey for 10 minutes, 14000rpm , centrifuged at 4°C for 10 min, and separated by liquid chromatography tandem high-resolution mass spectrometry. The liquid chromatography instrument conditions are: Thermo Scientific Hypersil GOLD C18 (100×2.1mm, 1.9μm) chromatographic column, column temperature 40°C, injection volume 5μL, 0.1% formic acid water as mobile phase A, acetonitrile as mobile phase B, Flow rate 0.3mL / min, using gradient elution program: 0-2min, 5% mobile phase B; 7min, 30% mobile phase B; 13-18min, 95% mobile phase B; 18.1-20min, 5% mobile phase B. The conditions of the high-resolution mass spectrometer are: spray voltage 3.5kV(+), 3.2kV(-); spray temperature 350°C, shea...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com