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Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof

A compound microbial inoculum and soil-borne disease technology, applied in the field of microorganisms, can solve the problems of single disease control, poor comprehensive effect, unstable effect, etc., and achieve the effect of simple process, maintaining ecological balance, and improving control effect.

Inactive Publication Date: 2020-07-10
SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the inventors have found that using a single bio-control bacterium to prevent and control diseases and insect pests not only prevents a single disease, but also has a poor overall effect, and requires a large amount of bacteria, resulting in an unstable effect.

Method used

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  • Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof
  • Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof
  • Composite microbial agent for preventing and treating soil-borne diseases and preparation method and application thereof

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preparation example Construction

[0053] In yet another specific embodiment of the present invention, the preparation method of the above-mentioned composite microbial bacterial agent is provided, and the preparation method includes:

[0054] Mix the original bacteria powder of the Paecilomyces lilacinus and Paenibacillus polymyxa evenly to obtain the composite original bacteria powder;

[0055] Mix and stir the adsorption mixture and the composite original bacteria powder evenly to obtain a mixture of microorganisms;

[0056] The above-mentioned mixture for adsorbing microorganisms and the synergistic mixture are uniformly mixed to obtain the composite microbial bacterial agent.

[0057] In yet another specific embodiment of the present invention, the original bacterial powder of Paecilomyces lilacinus and Paenibacillus polymyxa is obtained by fermentation.

[0058] In yet another specific embodiment of the present invention, the application of the above-mentioned composite microbial agent in preventing and ...

Embodiment 1

[0063] The preparation method of the original bacterial powder of embodiment 1 Paecilomyces lilacinus and Paenibacillus polymyxa

[0064] The preparation method of described Paecilomyces lilacinus comprises the following steps:

[0065] Step 1, culturing the slant strains, inoculating the original strains on the PDA slant, and putting the inoculated slant culture medium into a 28°C incubator for 14 days to obtain the first batch of slant strains.

[0066] Step 2. Prepare secondary liquid strains, each 1L of liquid medium contains: 3g NaNO 3 ,1g K 2 HPO 4 , 0.5gMgSO 4 ·7H 2 O, 0.5g KCl, 0.01g FeSO 4 , 30g sucrose and distilled water. Put the sterilized liquid medium into a 3L Erlenmeyer flask, fill each bottle with 0.7-0.8L liquid medium, and inoculate each bottle with a slant strain prepared in step 1 under aseptic conditions. After inoculation, The liquid culture medium is placed on a shaker and shaken for 24-48 hours at a temperature of 28° C. and a rotation speed of ...

Embodiment 2

[0074] The composite microbial bacterial agent for preventing and treating soil-borne diseases in the present embodiment is composed of the following components by weight: 1.5 parts of Paecilomyces lilacinus, 1.0 parts of Paenibacillus polymyxa, 4.2 parts of calcium lignosulfonate, carboxymethyl 4.2 parts of sodium cellulose, 10.4 parts of smoke foam, 8.4 parts of Fumei powder, 6.0 parts of starch, 8.4 parts of diatomaceous earth, 14.5 parts of compound synergist, 11.2 parts of attapulgite, and 30.2 parts of water.

[0075] The compound synergist is formed by mixing alginic acid, humic acid and chitin, and the mass ratio of the three is 1:1.5:1.

[0076] The preparation method of the composite microbial bacterial agent in the present embodiment, its steps are as follows:

[0077] 1, the preparation of former bacterial powder is with embodiment 1;

[0078] 2. Mix and stir the original bacteria powders of Paecilomyces lilacinus and Paenibacillus polymyxa to obtain a composite o...

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Abstract

The invention provides a composite microbial agent and a preparation method and application thereof, and belongs to the technical field of microorganisms. The composite microbial agent is prepared from the following components in parts by weight: 1-2 parts of paecilomyces lilacinus, 0.5-1.5 parts of paenibacillus polymyxa, 3-10 parts of calcium lignosulphonate, 2-8 parts of sodium carboxymethyl cellulose, 7-17 parts of tobacco powder, 8-15 parts of auxiliary beauty powder, 3-7 parts of starch, 8-16 parts of a composite synergist, 5-15 parts of diatomite, 7-14 parts of attapulgite and 23.5-35.5parts of water. Paecilomyces lilacinus and paenibacillus polymyxa are combined for use, soil-borne diseases and insect pests such as root-knot nematode, Chinese cabbage soft rot, pepper root rot, tomato seedling blight and pepper phytophthora blight can be effectively prevented and treated, and the product is non-toxic, pollution-free, safe and efficient and is beneficial to maintaining ecological balance and is an effective substitute for part of pesticides abd meets the requirements of agricultural sustainable development and has good practical application value.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a compound microbial bacterial agent for preventing and controlling soil-borne diseases, a preparation method and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Soil-borne diseases refer to diseases caused by pathogens such as fungi, bacteria, nematodes and viruses that live in the soil with diseased residues and attack crops from roots or stems when conditions are suitable. Chinese cabbage soft rot, pepper root rot, tomato blight and pepper blight are all soil-borne diseases, which have the characteristics of short disease cycle and hi...

Claims

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Application Information

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IPC IPC(8): A01N63/30A01N63/22A01N43/16A01N61/00A01P5/00A01P3/00C12N1/20C12N1/14C12R1/01C12R1/79
CPCA01N43/16A01N61/00A01N63/00C12N1/14C12N1/20
Inventor 单宝龙胡著然王丽荣张文娟齐素敏陈丹丹冉新炎韩广泉陶宁李圆圆黄悦谷巍程福亮徐海燕申小冉徐曙光李敏
Owner SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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