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Double-layer osteochondral tissue repair scaffold and preparation method thereof

A tissue repair, osteochondral technology, used in tissue regeneration, medical science, prosthesis, etc.

Active Publication Date: 2020-07-07
SHAANXI GIANT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These materials usually consist of separate cartilage layers and bone repair layers, and these scaffolds do not really address the repair of multiple layers of osteochondral tissue
Additionally, improving the stability of bilayer scaffolds and achieving a good interface between different layers remains a major challenge.

Method used

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  • Double-layer osteochondral tissue repair scaffold and preparation method thereof
  • Double-layer osteochondral tissue repair scaffold and preparation method thereof
  • Double-layer osteochondral tissue repair scaffold and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0040] The invention relates to a preparation method of a double-layer osteochondral tissue repair scaffold, the method comprising:

[0041] preparing a first feed solution, which includes recombinant collagen, sodium hyaluronate and hydroxyapatite;

[0042] Prepare a second feed liquid, which includes recombinant collagen and sodium hyaluronate;

[0043] freeze-drying the first feed liquid and the second feed liquid to form a gel-like double-layer structure;

[0044] A crosslinking agent is added to the gel-like bilayer structure for crosslinking.

[0045] Among them, recombinant collagen is a new type of genetically engineered protein. The mRNA of human collagen can be reverse-transcribed into cDNA by recombinant Escherichia coli BL21, which is introduced into Escherichia coli for high-density fermentation after enzyme digestion, specific suture and connection , and then obtained by separation and purification. In this specification, recombinant collagen refers to the rec...

Embodiment 1

[0064] The double-layer osteochondral tissue repair scaffold of this embodiment simulates the physiological structure and composition of natural osteochondral tissue. The upper layer uses recombinant collagen and sodium hyaluronate to repair the cartilage layer; the lower layer uses recombinant collagen and sodium hyaluronate. It is an organic phase, and nano-hydroxyapatite is used as an inorganic phase to repair the subchondral bone layer. After it is freeze-dried, it is prepared by cross-linking under the action of a cross-linking agent, and the cross-linking agent is 95% of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride weak.

[0065] The preparation method of the double-layer osteochondral tissue repair scaffold of this embodiment specifically includes:

[0066] (1) Prepare the first feed liquid, the first feed liquid includes recombinant collagen, sodium hyaluronate and hydroxyapatite. Specifically, the recombinant collagen, sodium hyaluronate and hydroxyap...

Embodiment 2

[0071] The difference between Example 2 and Example 1 is that in the first feed liquid, the concentration of recombinant collagen is 95 mg / mL, the molecular weight of recombinant collagen is 100 kD, the concentration of sodium hyaluronate is 9 mg / mL, and the concentration of sodium hyaluronate is 9 mg / mL. The molecular weight is 90kD, the concentration of hydroxyapatite is 45mg / mL; in the second feed solution, the concentration of recombinant collagen is 85mg / mL, the molecular weight of recombinant collagen is 100kD, the concentration of sodium hyaluronate is 9mg / mL, hyaluronic acid Sodium acid has a molecular weight of 90kD. Other reaction conditions are the same as in Example 1, see Table 1 for details.

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Abstract

The invention relates to a preparation method for a double-layer osteochondral tissue repair scaffold. The preparation method comprises the following steps: preparing a first feed liquid which comprises recombinant collagen, sodium hyaluronate and hydroxyapatite; preparing a second feed liquid which comprises recombinant collagen and sodium hyaluronate; freezing and drying the first feed liquid and the second feed liquid so as to form a gel-like double-layer structure; and adding a cross-linking agent into the gel-like double-layer structure for cross-linking. The invention also relates to thedouble-layer osteochondral tissue repair scaffold. The double-layer osteochondral tissue repair scaffold comprises a first layer and a second layer, wherein the first layer is prepared from raw materials including the recombinant collagen, the sodium hyaluronate and the hydroxyapatite; and the second layer is prepared from raw materials including the recombinant collagen and the sodium hyaluronate. The double-layer osteochondral tissue repair scaffold prepared by using the preparation method provided by the invention has excellent mechanical properties, good biocompatibility and proper degradation rate; and the scaffold material can be reused as a raw material for new osteogenesis after being degraded, so the repair of osteochondral tissues is realized.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a double-layer osteochondral tissue repair bracket and a preparation method thereof. Background technique [0002] Osteochondral defects are common disorders of the joints, including defects in articular cartilage and subchondral bone. There are many causes of osteochondral damage, such as sports injuries, traffic accidents, and various degenerative diseases. In addition, with age, the natural wear and tear of cartilage tissue can easily cause osteoarthritis, which is also the main cause of osteochondral damage. Due to physiological properties, cells in cartilage tissue can hardly regenerate, therefore, cartilage reconstruction is a great challenge. As cartilage damage progresses, it often extends to the subchondral bone, making repair of the cartilage and subchondral tissue necessary. [0003] Articular cartilage and subchondral bone are closely conne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/46A61L27/48A61L27/52A61L27/58C08J9/28C08J9/36C08L89/00C08L5/08C08K3/32
CPCC08J9/28C08J9/36A61L27/46A61L27/48A61L27/52A61L27/58C08J2389/00C08J2405/08C08J2201/0484C08K2003/325A61L2430/06C08L89/00C08L5/08B29C39/38A61L27/56C08L89/06A61L2430/02B29K2089/00B29L2031/7534
Inventor 范代娣刘凯强段志广马晓轩
Owner SHAANXI GIANT BIOTECHNOLOGY CO LTD
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