Freezing and unfreezing control system for human embryo cryopreservation

Abstract

The invention discloses a freezing and unfreezing control system for human embryo cryopreservation. The system comprises a box body, a computer main control system, a display system, a pipeline systemand a micro camera system are arranged in the box body, the computer main control system controls the whole process of embryo freezing and unfreezing, the pipeline system comprises a liquid nitrogenconveying pipe and a plurality of pipelines, one end of the liquid nitrogen conveying pipe is connected with a liquid nitrogen controller, one end of each one of the pipelines are connected with a refrigerating fluid storage bottle, an unfreezing fluid storage bottle and a waste liquid storage bottle, and the liquid nitrogen conveying pipe and the other ends of the pipelines are connected with a first needle type probe, a plurality of drawer type units are arranged on the inner wall of the box body, a micro cryopreservation device is arranged in the drawer type unit, a movable track is arranged in the box body, the micro camera system is arranged on the track and moves and rotates along the track at regular time so as to shoot embryo images in a micro cryopreservation device, and the shotembryo images are displayed through a display system. Integrated operation can be carried out, repeated transfer is not needed, and multiple embryos can be frozen at the same time.

Description

technical field [0001] The invention belongs to medical equipment, in particular to a control system for vitrification and thawing of human embryos. Background technique [0002] The birth of human embryo freezing and thawing technology is a milestone in the development of assisted reproduction. As early as 1983, Australian scholars reported the first case of pregnancy after frozen embryo transfer in history. Since then, embryo freezing and thawing technology has developed rapidly. In the treatment cycle of assisted reproduction, there are often redundant embryos that need to be preserved urgently, or due to the occurrence of complications after oocyte retrieval, fresh cycle transplantation cannot be carried out and whole embryos are urgently needed to be preserved. Embryo freezing and thawing technology solves the problem of embryo preservation , helps to reduce the multiple pregnancy rate and the incidence of ovarian hyperstimulation syndrome (OHSS), and makes each oocyte...

AI Technical Summary

Problems solved by technology

However, so far, there is no integrated operating system for vitrification technology. The freezing process is all done manually, which requires high technical personnel, and the cost of time and energy is too high because it cannot be completed in batches.

The two freezing methods require multiple transfers of embryos during the freezing process, which increases the risk of embryo loss; and both methods have the problem of cryopreservation carriers. At present, there are mainly closed carriers and open carriers. Open carriers are used in clinical practice. It is rarely used in the world, and the closed carrier is mainly the Crytop carrier. The closed carrier has the problems of long operation time, high technical requirements, and high price. optimal

[0004] The consumables used in the embryo vitrification process are ordinary petri dishes. Before freezing, the freezing liquid must be pre-warmed, and then sucked into the marked petri dish. The embryos to be frozen are removed from the culture system step by step according to the required It takes time to transfer to different freezing liquids and then to liquid nitrogen. For the safety of samples, only the embryos of the same patient can be frozen at a time. Even the embryos of the same patient can only be frozen at the same time due to time constraints. 3 embryos, for patients with multiple frozen embryos, it is often necessary to freeze them in batches, so the whole freezing process is time-consuming and labor-intensive

When thawing, it is necessary to take the embryo out of the liquid nitrogen storage tank, balance it in different gradients of thawing solution, and perform the reverse process of the freezing process, which also has the problem of time-consuming and labor-intensive

[0005] In summary, the current vitrification technology lacks an integrated operating system, and the embryos need to be transferred multiple times during the freezing process, which increases the risk of embryo loss. Multiple embryos need to be frozen in batches, which is time-consuming and labor-intensive. Operators are also prone to frostbite by liquid nitrogen during the process

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