Recombinant escherichia coli for producing N-acetyl-5-hydroxytryptamine, and construction method and application of recombinant escherichia coli

Abstract

The invention discloses recombinant escherichia coli for producing N-acetyl-5-hydroxytryptamine, and a construction method and application of the recombinant escherichia coli. The invention firstly discloses the recombinant escherichia coli, and compared with a receptor escherichia coli, the recombinant escherichia coli has the advantages that the expression level of a gene of a tryptophan derivative synthesis related protein is increased and / or the content of the tryptophan derivative synthesis related protein is increased and / or the activity of the tryptophan derivative synthesis related protein is increased, wherein the tryptophan derivative synthesis related protein is selected from at least one of phenylalanine hydroxylase, dihydromonophosphate reductase, 4[alpha]-hydroxytetrahydrobiopterin dehydratase, aspartate transaminase family proteins and N-acetyl transferase. The invention further discloses the construction method and application of the recombinant escherichia coli. The N-acetyl-5-hydroxytryptamine synthesis efficiency of the recombinant escherichia coli for producing the N-acetyl-5-hydroxytryptamine is obviously higher than synthesis efficiency of other existing strains, and the recombinant escherichia coli has great significance for industrial production and large-scale application of N-acetyl-5-hydroxytryptamine.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a recombinant Escherichia coli producing N-acetyl-5-serotonin and its construction method and application. Background technique [0002] N-acetyl-5-hydroxytryptamine (N-Acetyl-serotonin, C 12 h 14 N 2 o 2 ) is a derivative of tryptophan and is the precursor of melatonin synthesis. Its synthesis pathway widely exists in natural organisms. The classic synthesis pathway in cells is: tryptophan—5-hydroxytryptophan—5-hydroxytryptamine — N-acetyl-5-hydroxytryptamine. N-acetyl-5-hydroxytryptamine plays many important roles in organisms. As a precursor of melatonin, N-acetyl-5-hydroxytryptamine also has good antioxidant capacity, such as effective protection against photocarcinogenesis caused by ultraviolet light and DNA damage, while N-acetyl-5-hydroxytryptamine plays an important role in the recovery of brain injury / stroke / nerve damage, and has the function of a neuroprotective ...

AI Technical Summary

Problems solved by technology

Although the synthesis pathway of tryptophan derivatives is very clear, the proteins involved in it have low specific activity, and most of them are difficult to express in the existing mature expression system, basically forming inclusion bodies or the expression level is extremely low. Therefore, So far, there are no engineering strains with high production of N-acetyl-5-hydroxytryptamine

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