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Fertile medium for boletus mycelium, and preparation method and application purification method of fertile medium

A purification method, the technology of boletus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fungi, etc., can solve the aging growth rate of mycelium mother species, mycelium not extending into the medium, slowness, etc. problems, to achieve the effect of promoting cultivation and artificial cultivation, and improving the survival rate

Active Publication Date: 2020-06-19
秦小波
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Zhang Chunxia (Analysis of the nutritional components of the fruiting body of Dictyostelium obliquus [J]. Journal of Yunnan University (Natural Science Edition), 2010, 32(6).) once screened the best M1 medium, the formula is glucose 20g; MgSO 4 1g; KH 2 PO 4 1g; Potato 200g; Agar 20g; Water 1L; Yeast extract 2g, but the mycelium of the mother species of mycelium is still aging and the growth rate is slow, and the mycelium only grows in the tissue block and does not extend into the medium

Method used

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  • Fertile medium for boletus mycelium, and preparation method and application purification method of fertile medium
  • Fertile medium for boletus mycelium, and preparation method and application purification method of fertile medium
  • Fertile medium for boletus mycelium, and preparation method and application purification method of fertile medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) prepare several groups of boletus mycelia fertile medium for subsequent use:

[0042]Weigh 150g of peeled potatoes, add water and boil for 20 minutes, filter the potato extract with gauze, then add 15.0g of glucose, 5mL of white radish juice, 1.0g of ammonium chloride or ammonium nitrate, MgCl 2 1.0g, KCl 1.0g, sodium butyrate 0.5g and agar 20.0g, and finally add deionized water to make the volume to 1000mL to prepare a mixed solution;

[0043] Sterilize the mixture at 121°C for 20 minutes, then cool it to 65°C, add ampicillin and streptomycin so that the final concentration of both ampicillin and streptomycin is 20 μg / mL, mix well and immediately pour the plate to prepare Obtain boletus mycelia fertile culture medium;

[0044] 2) Mycelia isolation and cultivation: adopt the tissue separation method to isolate aseptic fruiting body tissue pieces or filaments from the fruiting bodies of Dictyostelium phalaensis, and quickly inoculate them on the fertile medium for Bo...

Embodiment 2

[0052] 1) prepare several groups of boletus mycelia fertile medium for subsequent use:

[0053] Weigh 200g of peeled potatoes, add water and boil for 30 minutes, filter the potato extract with gauze, then add 20.0g of glucose, 10mL of white radish juice, 2.0g of ammonium chloride or ammonium nitrate, MgCl 2 2.0g, KCl 2.0g, sodium butyrate 1.5g and agar 30.0g, and finally add deionized water to make the volume to 1000mL to prepare a mixed solution;

[0054] Sterilize the mixture at 121°C for 20 minutes, then cool it to 50°C, add ampicillin and streptomycin to make the final concentration of both ampicillin and streptomycin 150 μg / mL, mix well and immediately pour the plate to prepare Obtain boletus mycelia fertile culture medium;

[0055] 2) Mycelia isolation and cultivation: adopt the tissue separation method to isolate aseptic fruiting body tissue pieces or filaments from the fruiting bodies of Dictyostelium phalaensis, and quickly inoculate them on the fertile medium for Bo...

Embodiment 3

[0063] 1) prepare several groups of boletus mycelia fertile medium for subsequent use:

[0064] Weigh 175g of peeled potatoes, add water and boil for 25 minutes, filter the potato extract with gauze, then add 17.5g of glucose, 7.5mL of white radish juice, 1.5g of ammonium chloride or ammonium nitrate, MgCl 2 1.5g, 1.5g KCl, 1g sodium butyrate and 25.0g agar, and finally add deionized water to make the volume to 1000mL to prepare a mixed solution;

[0065] Sterilize the mixture at 121°C for 20 minutes, then cool it to 60°C, add ampicillin and streptomycin to make the final concentration of both ampicillin and streptomycin are 50 μg / mL, mix well and immediately pour the plate to prepare Obtain boletus mycelia fertile culture medium;

[0066] 2) Mycelia isolation and cultivation: adopt the tissue separation method to isolate aseptic fruiting body tissue pieces or filaments from the fruiting bodies of Dictyostelium phalaensis, and quickly inoculate them on the fertile medium for ...

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Abstract

The invention relates to the technical field of tissue culture of strains, and discloses a fertile medium for boletus mycelium. The fertile medium includes a potato extracting solution, glucose, whiteradish juice, ammonium chloride or ammonium nitrate, MgCl2, KCl, sodium butyrate, agar and water. A preparation method and an application purification method of the medium are also disclosed. Thus, various wild boletus cells can be easily screened and grow on the medium, so that the dependence on wild environment and conditions can be avoided, the culture and artificial cultivation of boletus strains can be promoted, and the survival rates of the boletus strains can be enhanced.

Description

technical field [0001] The invention relates to the technical field of bacterial strain tissue culture, in particular to a boletus hyphae fertile culture medium, a preparation method and an application purification method thereof. Background technique [0002] Phlebopus portentosus belongs to the genus Phlebopus in the family Boletaceae of the order Boletales, and is mainly distributed in tropical regions. It is mainly distributed in Thailand, Vietnam, Indonesia, Brazil, Mexico, Australia, New Zealand and other regions abroad, and mainly distributed in Yunnan, Guangxi, Hainan and other places in China. Dictyostelium boletus has rich nutritional value, rich in protein, crude fat, total sugar, crude fiber, amino acid and various mineral elements such as potassium, calcium, magnesium, iron, zinc and so on. [0003] The carbon sources that can be used by the mycelia of Dictyostelium phelicus include glucose, fructose, lactose, sucrose, maltose, mannitol, malt extract, soluble s...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02C12R1/645
CPCC12N1/14C12N1/02
Inventor 秦小波
Owner 秦小波
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