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Lactobacillus plantarum with weight reducing function and application thereof

A technology of Lactobacillus plantarum and function, applied in the field of microorganisms, can solve the problems of obvious differences in individual intestinal flora and physiological state, and achieve the effects of reducing fat accumulation, inhibiting fat synthesis, and lowering blood lipids

Active Publication Date: 2020-06-09
HANGZHOU WAHAHA TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effects of these different probiotics are strain-specific, and individual intestinal flora and physiological state are significantly different, and the same probiotic may not be applicable to all individuals
Therefore, there is still a need to develop more new strains of probiotics that can control body weight and reduce fat accumulation to fill the gaps of existing strains (although most new strains themselves also have specificity, there may be such a situation: existing strains have specific effects on An individual does not work and the new strain happens to work)

Method used

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  • Lactobacillus plantarum with weight reducing function and application thereof
  • Lactobacillus plantarum with weight reducing function and application thereof
  • Lactobacillus plantarum with weight reducing function and application thereof

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Experimental program
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Embodiment 1

[0042] The bacterial strain provided by the present invention is identified as belonging to Lactobacillus plantarum (Lactobacillus plantarum), named 1701, and was preserved in the General Microorganism Culture Collection Center of China Microbiology Culture Collection Management Committee on October 23, 2019, and the microorganism preservation number is CGMCC NO. 18728.

[0043] The bacterial strain provided by the present invention is isolated by the inventor from yogurt powder sample collected in the village of Shigatse City, Tibet Autonomous Region of my country.

[0044] The biological properties of bacterial strain Lactobacillus plantarum 1701 of the present invention are as follows:

[0045] Morphological characteristics: In MRS agar medium, the growth form is milky white, opaque, round, smooth and moist surface, neat edges, and centrally raised colonies. Gram staining was typically positive, and the cells were observed under a microscope to be long rod-shaped, without ...

Embodiment 2

[0053] Lactobacillus plantarum 1701 of the present invention, reference commercial bacterial strain Lactobacillus rhamnosus GG (LGG) and Lactobacillus casei Daita strain (LcS) after second-generation activation, get the bacterial liquid at the end of logarithmic growth, centrifuge at 4000rpm for 10min, discard the supernatant, To obtain the bacteria sludge, perform the following operations respectively: ①Add the same volume of MRS solution with pH 2.5, blow and mix well, incubate at 37°C, and measure the number of bacteria after incubation for 0h, 1h, 2h, and 4h by the dilution coating counting method Change; ② Add the same volume of MRS solution containing 0.3% bile salts, pipette and mix well, incubate at 37°C, and measure the changes in the number of bacteria after incubation for 0h, 4h, and 8h by the dilution coating counting method. Strain survival rate calculation formula:

[0054] Strain survival rate (%)=N1 / N0×100%.

[0055] N1 is the number of viable bacteria after t...

Embodiment 3

[0061] The HT-29 cell culture system was established, and the cells were grown in DMEM medium containing 10% fetal bovine serum (containing penicillin 100 U / mL, streptomycin 100 mg / mL). After the cells were transferred to the third passage, they were digested with 0.25% trypsin (containing EDTA) to obtain a single cell suspension, and the cells were divided into 1×10 6 The density of cells / well was seeded in a 12-well cell culture plate with cell slides placed, at 37°C, 5% CO 2 Cultured in the incubator for 2 days.

[0062] After the bacterial strain Lactobacillus plantarum 1701 of the present invention, the contrast commercial bacterial strain Lactobacillus rhamnosus GG (LGG) and Lactobacillus casei Daita strain (LcS) are activated by the second generation, take the bacterial liquid at the end of logarithmic growth, centrifuge at 4000rpm for 10min, discard the supernatant , to obtain the bacteria sludge, resuspended in DMEM complete medium containing 10% fetal bovine serum (...

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Abstract

The invention relates to the field of microorganisms and discloses lactobacillus plantarum with a weight reducing function and application thereof. The lactobacillus plantarum 1701 strain is separatedfrom a yogurt powder sample collected from rural areas of Shigatse in the Tibet Autonomous Region. The strain is preserved in the China General Microbiological Culture Collection Center on October 23, 2019, and the microorganism has the preservation number of CGMCC NO.18728. The lactobacillus plantarum 1701 has excellent tolerance and adhesion, can remarkably reduce the weight of a rat, reduce fat accumulation in a body, reduce the viscera ratio and body fat ratio, reduce the leptin level and reduce blood lipid and can be used in medicines, health-protecting products and health foods for preventing and treating obesity. Furthermore, the strain still has a weight reducing function after inactivation, thus having rich product mode, wide application range, high product stability and long preservation period.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a strain of Lactobacillus plantarum with weight loss function and its application. Background technique [0002] Obesity is mainly due to the disorder of the body's energy metabolism balance, resulting in energy intake exceeding energy consumption, resulting in excessive accumulation of body fat, resulting in metabolic diseases. Obesity is not only affected by multiple factors such as genetics, environment, metabolism, and physiology, but is also associated with diseases such as hyperlipidemia, hypertension, diabetes, insulin resistance, hyperuricemia, non-alcoholic fatty liver, and coronary atherosclerotic heart disease. Closely related. At present, obesity and its complications have become a major public health problem worldwide, which not only affects the appearance of individuals, but also poses serious hazards to human health. [0003] At present, a large number of studies ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23C9/123A23C19/032A23C19/06A23L33/135A61K35/747A61P3/04C12R1/25
CPCC12N1/20A23C9/1234A23C19/0323A23C19/062A23L33/135A61K35/747A61P3/04A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2200/3262A23V2200/332
Inventor 孙盛陈苏陈丽娥俞赟霞李言郡陈彩玲郑志瑶陈作国任学良朱珺
Owner HANGZHOU WAHAHA TECH
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