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Tricholoma bakamatsutake culture medium and application thereof

A technology of culture medium and liquid culture medium, which is applied in the field of microbial cultivation, and can solve problems such as difficult bionic cultivation and slow artificial cultivation

Active Publication Date: 2020-06-09
SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the biomimetic cultivation method needs a large amount of mycelium propagated on the artificial medium, and the slow artificial cultivation of Quercus matsutake mycelium restricts the large-scale biomimetic cultivation of this fungus.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0028] Based on the mass parts of the potato powder, the liquid medium of the present invention further preferably includes 0.6 to 1.4 parts of dried fruit powder, more preferably 0.7 to 1.3 parts. The dried fruit powder preferably includes kiwi fruit powder, Acanthopanax senticosus powder and rose petal powder, and the mass ratio of the kiwi fruit powder, Acanthopanax senticosus powder and rose petal powder is preferably 70-80:15-28:2~ 5. It is more preferably 70-80:20:2-5; the particle size of the dried fruit powder is preferably less than 0.85 mm. In the present invention, the preparation method of the dried fruit powder preferably includes: drying, pulverizing and sieving the dried fruit kiwi fruit, Acanthopanax senticosus and dried rose petals in sequence to obtain the dried fruit powder; and the drying temperature It is preferably 40-60°C, and the time is preferably 48-72h; the sieve for sieving is preferably a 20-mesh sieve. The function of dried fruit powder is to prov...

Embodiment 1

[0041] Take the soft jujube kiwi fruit, acanthopanax senticosus fruit and rose petals and dry them in an oven at 60°C for 72 hours, crush them and mix them, pass through a 20-mesh sieve to obtain dried fruit powder;

[0042] Take 32g of dry potato powder, 4g of malt extract, 2g of yeast powder, 2g of peptone, 1g of dried fruit powder mixture (containing 0.8g of jujube kiwi powder, 0.15g of acanthopanax powder, 0.05g of dried rose petal powder), 1g of ammonium tartrate, lemon Acid iron 5mg, add 1000ml of water, pH value 4.5, stir evenly, boil to fully dissolve the nutrients of each component to obtain a liquid medium;

[0043] Take 100ml liquid culture medium into 250ml Erlenmeyer flask, seal it with cotton stopper, sterilize it at 121℃ for 20 minutes, take it out and cool to room temperature, according to the ratio of hypha mass to liquid culture volume ratio of 2g:100ml, The mycelium of Matsutake quercus was inoculated in a liquid medium, and the culture was shaken at 120 rpm for ...

Embodiment 2

[0047] Take the soft jujube kiwi fruit, acanthopanax senticosus fruit, and rose petals, and dry them in an oven at 60°C for 72 hours, and crush them through a 20-mesh sieve to obtain various dried fruit powders;

[0048] Take 200g of peeled fresh potatoes and chop them, add 1000ml of water, boil until there are no solids, filter with 3 layers of gauze, add 4g of malt extract, 2g of yeast powder, 2g of peptone, 1g of ammonium tartrate, 5mg of ferric citrate, and 1g of dried fruit powder ( 0.7g of soft jujube kiwi powder, 0.28g of acanthopanax powder, 0.02g of dried rose petal powder), stir evenly, pH value of 4.5, to obtain a liquid medium;

[0049] Take 120ml of the obtained liquid culture medium into a 250ml Erlenmeyer flask, seal it with cotton stopper, sterilize at 121℃ for 20 minutes, cool to room temperature, according to the ratio of the hypha mass to the volume ratio of the liquid medium of 3g: 120ml, The mycelium of Matsutake kucherii was inoculated into a liquid medium, an...

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Abstract

The invention belongs to the technical field of microorganism culture, and in particular relates to a tricholoma bakamatsutake culture medium and a preparation and an application thereof. The tricholoma bakamatsutake culture medium provided by the invention comprises a liquid culture medium, wherein the liquid culture medium comprises the following components in parts by mass of 30-35 parts of potato powder, 3-5 parts of malt extract powder, 1-3 parts of yeast powder, 1-3 parts of peptone, 0.5-1.5 parts of ammonium tartrate, 0.004-0.006 parts of ferric citrate, 0.5-1.5 parts of dried fruit powder, and 1000 parts of water. The tricholoma bakamatsutake culture medium enables the growth vitality of the tricholoma bakamatsutake mycelium to be increased in the artificial culture condition, themycelium biomass is increased, and the mycelium culture cycle can be shortened, thereby obtaining a large amount of tricholoma bakamatsutake mycelium to be applied to large-scale culture study and field bionic cultivation inoculation, so that the more efficient fast culture is realized.

Description

Technical field [0001] The invention belongs to the technical field of microbial culture, and specifically relates to a culture medium for Quercus matsutake and its application. Background technique [0002] Quercus matsutake is the fruiting body of a large fungus of the genus Tricholoma, the Latin scientific name: Tricholoma bakamatsutake. Quercus matsutake is a precious edible and medicinal fungus. It contains 18 amino acids, 14 essential trace elements for the human body, 49 active nutrients, and 5 There are two kinds of unsaturated fatty acids, 8 kinds of vitamins, rich dietary fiber and a variety of active enzymes. It also contains double-stranded matsutake polysaccharides, matsutake polypeptides and matsutake alcohol. It is a world-renowned natural edible and medicinal fungus. Matsutake is not only balanced and adequate in nutrition, but also has multiple effects such as improving immunity, anti-cancer and anti-tumor, curing diabetes and cardiovascular diseases, and anti-ag...

Claims

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Application Information

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IPC IPC(8): A01G18/20A01G18/40A01G18/00
CPCA01G18/20A01G18/40A01G18/00
Inventor 袁海生魏玉莲曹婷俞嘉瑞
Owner SHENYANG INST OF APPLIED ECOLOGY - CHINESE ACAD OF SCI
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