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Tangerine product having functions of improving intestinal microflora structure and alleviating atherosclerosis as well as preparation method and application thereof

A technology for atherosclerosis and intestinal flora, applied in the food field, can solve the problems of low extraction rate and narrow application scope, and achieve the effect of increasing the extraction rate, expanding the application scope and improving the structure of intestinal flora.

Inactive Publication Date: 2020-06-05
INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction of functional flavonoids from fruit peel residues currently has the problems of low extraction rate and narrow application range.

Method used

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  • Tangerine product having functions of improving intestinal microflora structure and alleviating atherosclerosis as well as preparation method and application thereof
  • Tangerine product having functions of improving intestinal microflora structure and alleviating atherosclerosis as well as preparation method and application thereof
  • Tangerine product having functions of improving intestinal microflora structure and alleviating atherosclerosis as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Embodiment 1, preparation and purification of citrus products

[0055] 1) After cleaning the pomelo peel, cut it into small pieces, freeze at -20°C for 48 hours, grind and crush it into powder under liquid nitrogen conditions, and store it in a -20°C refrigerator.

[0056] 2), using a mixed aqueous solution of ethanol and acetic acid to heat and ultrasonically assist leaching method to extract naringin, weigh the powder in step 1), and the ratio of solid to liquid is 1:30g / mL. In the mixed aqueous solution, the concentration of ethanol is 60%, adding acetic acid to make the final concentration 5%, constant temperature at 75°C, leaching with ultrasonic assistance for 3h, filtering with a 200-mesh filter cloth to remove the residue, and obtaining a flavonoid liquid.

[0057] 3) Evaporate and concentrate the flavonoid liquid obtained in step 2), and obtain a certain concentration of naringin mixture after removing ethanol, and obtain a naringin extraction rate of 6.2%.

[0...

Embodiment 2

[0063] Embodiment 2, the preparation of citrus flavone fruit juice

[0064] Juice refers to freshly squeezed fresh fruits, compound juice drinks or compound drinks.

[0065] The fruit juice described in this embodiment refers to the juice mainly freshly squeezed from watermelon.

[0066] 1) Take fresh watermelon, peel, remove seeds, cut into pieces, and squeeze the juice.

[0067] 2) Filter the juice obtained in 1) with a 200-mesh filter cloth to remove residue and collect the juice.

[0068] 3) Divide the juice obtained in 2) into several parts, add a certain amount of citrus flavonoids (naringin) respectively, and prepare citrus flavonoids+ watermelon juice,

[0069] 4) Since some citrus flavonoids have a bitter taste, add 2-3g of white sugar per 100mL of fruit juice to adjust the taste.

[0070] 5) The juice obtained in 4) is homogenized under high pressure, and the pressure is 25MPa.

Embodiment 3

[0071] Example 3, the application of citrus flavonoid products in regulating intestinal flora and protecting cardiovascular food

[0072] The fruit juices containing 0%, 0.125%, 0.25% and 0.5% of the citrus flavonoid naringin mass fraction prepared in Example 2 were used for experiments.

[0073] Select 32 male C57Bl / 6J mice with similar body weight at the age of 8 weeks, and divide them into 4 groups / 8 at random according to body weight, respectively control group (0%), naringin (0.125%), naringin ( 0.25%) and naringin (0.5%). The rats were given a normal diet and fed fruit juices with different concentrations of naringin by oral gavage, once a day, for 4 weeks. At the end of the experiment, feces were taken in a sterile environment, quickly put into liquid nitrogen for storage, and serum and other tissues were collected and stored.

[0074] Further, 20 male C57Bl / 6J mice with similar body weight at the age of 8 weeks were randomly divided into 2 groups / 10, blank group (0%)...

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Abstract

The invention discloses a tangerine product having functions of improving intestinal microflora structure and alleviating atherosclerosis as well as a preparation method and application thereof. The preparation method of the tangerine product comprises the following steps: making pomelo peels into pomelo powder; extracting the pomelo powder by adopting a mixed aqueous solution of ethanol and acetic acid, filtering the pomelo powder and removing residues to obtain flavone liquid; concentrating the flavone liquid to obtain rough naringin mixture, and performing separation and purification to obtain tangeritin; and mixing the tangeritin with auxiliaries of food or medicines to obtain a final product. The tangerine product disclosed by the invention can regulate intestinal microflora or alleviate atherosclerosis; an experiment proves that naringin fruit juice with different concentration can change proportions of the flora bacteroidetes and thick-walled bacteroidetes, and the concentrationis increased dependently. According to the tangerine product disclosed by the invention, fine and further processing of the tangerine is promoted, the biological activity of functional flavone is fully utilized, the intestinal microflora structure is improved, the healthy metabolism of food and drink containing cholesterol is promoted, occurrence of the atherosclerosis is effectively alleviated,and the healthy state of a body is improved.

Description

technical field [0001] The invention relates to a citrus product with the functions of improving intestinal flora structure and slowing atherosclerosis, a preparation method and application thereof, and belongs to the field of food. Background technique [0002] Intestinal flora is a general term for the large number and variety of microorganisms that reside in the human gastrointestinal tract. The distribution of intestinal bacteria in the gastrointestinal tract increases sequentially from top to bottom, and more than 90% of them are distributed in the colon, and the number reaches 10 11 ~10 12 . The human intestinal flora is huge. It not only participates in the metabolism of nutrients, but also participates in the human intestinal immunity, the growth and differentiation of intestinal epithelial cells, and the formation of chronic diseases such as atherosclerosis and other cardiovascular and cerebrovascular diseases. The missing micro-ecosystem is closely related to hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L33/105A23L2/06A23L2/60A61K9/20A61K9/48A61K31/352A61P1/00A61P9/10
CPCA23L33/105A23L2/06A23L2/60A61K31/352A61K9/2059A61K9/4866A61P1/00A61P9/10A23V2002/00A23V2200/32A23V2200/326A23V2200/30A23V2250/21162
Inventor 郑金铠王凤王凤忠赵成英范蓓田桂芳陆畅
Owner INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
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