Algae and bacterium mixed preparation capable of improving water and manure holding effect and application

A technology of mixing bacterial agents and algae bacteria, applied in the agricultural field, can solve the problems of less research and application, and achieve the effects of broad application prospects, enhanced stability, and improved lasting effect.

Inactive Publication Date: 2020-06-02
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the applications of artificial biological crusts are aimed at single algal crusts or single fungal crusts, and there are few researches and applications on bacterial crusts, especially the mixed biological crusts of bacteria and algae

Method used

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  • Algae and bacterium mixed preparation capable of improving water and manure holding effect and application
  • Algae and bacterium mixed preparation capable of improving water and manure holding effect and application
  • Algae and bacterium mixed preparation capable of improving water and manure holding effect and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] The strain Sphingomonas TJA 3-1 (disclosed in the patent publication CN109988731A, Sphingomonas TJA3-1, the name is TJA 3-1, the classification name is Sphingomonas sp., and the preservation number is: CGMCC No.15057, date of preservation: December 13, 2017, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, preservation unit: General Microbiology Center of China Committee for the Collection of Microbial Cultures.) and Bacillus coli LB culture medium for activation on the slant, take a freshly activated slant strain, add 5mL of sterile water, mix evenly, and insert them into 250mL shake flasks containing 100mL of fermentation medium respectively, the composition of the fermentation medium (g / L ): Sucrose 30.0, Sodium Nitrate 2.0, K 2 HPO 4 0.5, MgSO 4 0.2, FeSO 4 0.01, pH 7.0. Place in a shaker at 30°C, shake at 150r / min for 60h, add sterile water, and dilute the cell concentration of the fermentation liquid of Sphingomonas TJA 3-1 and Bacillus colloid...

Embodiment 2

[0057] Insert Chlorococcus and Nostoc into the fermentation culture at 5% inoculum size (g / L): Sodium Nitrate 1.5, K 2 HPO 4 0.03, MgSO 4 0.05, CaCl 2 0.01, ferric ammonium citrate 0.005, pH 7.0, placed in a light incubator, constant temperature 20°C, sunlight light intensity 4000Lux, light cycle 16h:8h, cultured for 20 days, collected algae cells and freeze-dried, mixed at 1:1 to obtain Mix the algae agent and dilute to a concentration of 0.5% before use.

[0058] The detection method of water retention effect is with embodiment 1, and result is as follows figure 1 As shown, it can be seen that after spraying the mixed algae agent, the water retention effect of the soil was significantly improved, and the water in the control soil sample was completely evaporated in 10 days. At this time, the moisture content of the soil sample sprayed with the mixed agent was 0.49%.

[0059] The detection method of fertilizer-retaining effect is with embodiment 1, and result is as foll...

Embodiment 3

[0062] Mix the mixed bacterial agent of Example 1 with the mixed algal agent of Example 2 at a ratio of 1:1 to obtain a mixed algae-bacteria preparation. The detection method of water retention effect is with embodiment 1, and result is as follows figure 1 As shown, it can be seen that after spraying the mixed algae agent, the water retention effect of the soil is significantly improved, and the water in the control soil sample is completely evaporated in 10 days. The water retention effect is 1.35 and 9.08 times that of Examples 1 and 2.

[0063] The detection method of fertilizer-retaining effect is with embodiment 1, and result is as follows figure 2 and 3 As shown, after the application of mixed algal agents, the soil's fertilizer retention capacity is improved, and in the experiment of leaching simulated rainfall, the leaching of nitrogen, phosphorus and potassium fertilizers is reduced, and the NH in the first soil leaching solution 4 + 、PO 4 3- 、K + The content ...

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Abstract

The invention relates to an algae and bacterium mixed preparation capable of improving water and manure holding effect. The algae and bacterium mixed preparation consists of the following raw materials in the following compounding ratio of components of a mixed bacterium agent containing sphingomonas xenophaga TJA 3-1 and bacillus mucilaginosus, and a mixed algae agent containing chlorococcum sp.and nostoc punctiforme, wherein the mixed bacterium agent and the mixed algae agent are mixed in the volume ratio of being ( 3 to 1) to (1 to 2), so that the algae and bacterium mixed preparation is obtained. The algae and bacterium mixed preparation is applied to soil, so that compact biology breadcrust can be formed, the water and manure holding effect of the soil can be increased, and growth ofseedlings of cotton and the like can be promoted. Compared with other artificial biology breadcrust, algae and bacteria form a microorganism syntaxial system, through interaction of the algae and thebacteria, the persistent bioactivity of the preparation is improved, the problems that the algae breadcrust is slow in shaping, low in persistent bioactivity and the like, are solved, and the algae and bacterium mixed preparation is suitable for large-scale production and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of agriculture, in particular to a mixed preparation of algae and bacteria for improving the water and fertilizer retention effect and its application. Background technique [0002] Soil desertification is a very serious ecological and environmental problem. Desertification leads to changes in soil properties and structures, and a decrease in water and fertilizer retention capacity, which in turn affects the growth of plants. The types and quantities of soil microorganisms will also be significantly reduced, gradually changing the soil redox state, the relationship between soil-plant-microbes enters a vicious circle, and the process of anti-soil formation begins. With wind erosion, the loss of surface soil is exacerbated, and the soil begins to desertify. Left unchecked, it will eventually lead to the disappearance of fertile soil and desertification. area increases. Methods to prevent and control soil dese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/12C09K17/14C12R1/07C12R1/89C12R1/01C09K101/00
CPCC09K17/14C09K2101/00C12N1/12C12N1/20
Inventor 黄海东杨海霞李晓雁任梦楠杨红澎权桂芝
Owner TIANJIN AGRICULTURE COLLEGE
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