Diatomite-beet armyworm nuclear polyhedrosis virus composition and preparation thereof
A technology of karyotype polyhedrosis and beet armyworm, applied in the field of agrochemical, can solve the problems of being easily affected by weather factors, difficult to form uniform dispersion, affecting the use effect, etc., so as to shorten the onset time, improve the binding force, and achieve targeted strong effect
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Embodiment 1
[0050] Co-toxicity coefficient method (CTC) screening: combined toxicity determination of diatomite and beet armyworm nuclear polyhedrosis virus to Spodoptera litura; the diatomaceous earth has a sawtooth structure (French Iridite, Celite610).
[0051] Referring to the method in the NY / T1154.14-2008 standard, the method of soaking leaf discs is adopted. Use a hole puncher with a diameter of 18mm to punch the cabbage leaves into leaf discs, immerse the leaf discs in the test drug solution for 10 seconds, and put them into a petri dish (90mm in diameter) with a moisture-retaining filter paper, and put 6 discs in each dish. After the leaf discs are dried naturally, select the 3rd instar larvae of Spodoptera litura that have been hungry for 4 hours and put them in the petri dish, with no less than 10 larvae in each dish, and repeat 4 times. After the treatment is completed, cover the dish lid and place it in the observation room to cultivate.
[0052] After 7 days, the death of t...
Embodiment 2
[0061] Co-toxicity coefficient method (CTC) screening: the combined toxicity of diatomite and beet armyworm nuclear polyhedrosis virus to the tea geometrid; the diatomite has a sawtooth structure (French imeryt, Celite610).
[0062] Referring to the method in the NY / T1154.14-2008 standard, the leaf soaking method is adopted. Select 3 buds of fresh tea trees, soak them in the test drug solution for 30 seconds, take them out to dry naturally, wrap the base of the buds with wet absorbent cotton to keep moisture, then seal the absorbent cotton balls with plastic wrap, put them in canned bottles, and The concentration was repeated 4 times. Then use a writing brush to insert lively and healthy 2nd instar nymphs of tea geometrids of the same size, 15 ± 2 heads per can, and seal them with white gauze. After processing, put it in the observation room.
[0063] After 7 days, the death of the test insects was checked and recorded. The standard for judging the death of the test insects ...
Embodiment 3
[0072] Embodiment 3 provides a kind of diatomite-beet armyworm nucleopolyhedrosis virus wettable powder, and its preparation raw material comprises: dispersant 6wt%, wetting agent 4wt%, synergist 5wt%, filler 10wt%, remaining Quantities are diatomaceous earth-beet armyworm nuclear polyhedrosis virus composition.
[0073] The diatomaceous earth-beet armyworm nuclear polyhedrosis virus composition comprises diatomaceous earth and beet armyworm nuclear polyhedrosis virus former medicine; The beet armyworm nuclear polyhedrosis virus former medicine is 200 billion PIB / gram The former powder of beet armyworm nuclear polyhedrosis virus was purchased from Baiyun Industrial Co., Ltd., Jiyuan, Henan Province; the diatomaceous earth has a sawtooth structure, produced by the French Imeryste Company, and the brand is Celite610; the diatomite and beet nightworm The weight ratio of the original drug of moth nuclear polyhedrosis virus is 400:1.
[0074] The dispersant is a mixture of Morwet ...
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