Method for detecting content of isoorange flavones in blood plasma

A technology of isoartin and detection method, which is applied in the field of detection of isoarin content in plasma, and can solve problems such as inability to quantitatively detect isoarin

Active Publication Date: 2020-05-26
THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

According to research reports, methods such as liquid chromatography, ultraviolet spectroscopy, NMR, and liquid chromatography-mass spectrometry have been used for the detection of isoagarin in citrus extracts, but these methods cannot quantitatively detect isoaurin in biological samples

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  • Method for detecting content of isoorange flavones in blood plasma
  • Method for detecting content of isoorange flavones in blood plasma
  • Method for detecting content of isoorange flavones in blood plasma

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Embodiment 1

[0027] 1. Instruments and reagents

[0028] 1) Analytical instrument

[0029] The liquid mass spectrometer is an Agilent 1290UHPLC-6420 triple quadrupole mass spectrometer system, equipped with a 1290 quaternary pump, a 1290 autosampler, a 1260 column oven, an electrospray ion source and a MassHunter workstation. The chromatographic column is an Agilent ZORBAX Eclipse Plus C18 RRHD column.

[0030] 2) Reagent

[0031] Isoagarin and keratophyllin were provided by Chengdu Mansite Biotechnology Co., Ltd., and the content was greater than 99%. Methanol, acetonitrile and formic acid were chromatographically pure, other reagents were analytically pure, and water was prepared by a Millipore water purifier.

[0032] 2. Experimental methods and results

[0033] 1) Solution preparation

[0034] Preparation of standard solution: Accurately weigh about 1 mg of isoaurin standard product, and dissolve it in a certain amount of methanol to obtain 1 mg / mL isoaurin mother solution. Dilute...

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Abstract

The invention provides a method for detecting the content of isoorange flavone in blood plasma, and belongs to the field of pharmaceutical analysis. The method for detecting isoorange flavones in blood plasma based on a precipitation method and a liquid chromatography-mass spectrometry technology can be used for detecting the concentration of the isoorange flavone in a living body; a reliable analysis means is provided for clinical and basic research; according to the invention, a plasma sample containing isoorange flavones is treated by using a protein precipitation method; impurities and endogenous interferents are removed through a one-step precipitation reaction, and a certain amount of formic acid is added into a mobile phase in the separation process of high performance liquid chromatography, so that isoorange flavone forms more hydrogenated excimer ion peaks after ionization, the abundance of corresponding characteristic fragment ions is increased accordingly, and the detectionsensitivity is improved; in the quantification process, the lysionotin is used as an internal standard substance, and the isoorange flavone is quantitatively detected through specific and high-sensitivity monitoring of excimer ions from quasi-molecular ion peaks.

Description

technical field [0001] The invention relates to the technical field of drug analysis, in particular to a method for detecting the content of isoaurin in blood plasma. Background technique [0002] Isoatrienone is a polymethoxylated flavone, which widely exists in a variety of citrus plants and has a variety of biological activities. Molecular formula is C 20 h 20 o 7 , the molecular weight is 372.1. Isoaurinones have multiple pharmacological activities, including antitumor, antiproliferative, and antioxidant effects. In addition, there may be drug interactions between isoagarin and other drugs, and it can increase the plasma drug concentration of some drugs when taken at the same time. The research on polymethoxylated flavones represented by isoagarin is increasing day by day, and the determination of its plasma drug concentration in vivo plays an important role in evaluating drug efficacy and analyzing pharmacological effects. According to research reports, methods su...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/04G01N30/06G01N30/34G01N30/72
CPCG01N30/02G01N30/04G01N30/06G01N30/34G01N30/7266G01N2030/027G01N2030/045G01N2030/067
Inventor 叶炜剑牛超孙佳陈根黄晓忠
Owner THE SECOND HOSPITAL AFFILIATED TO WENZHOU MEDICAL COLLEGE
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