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Cross-linking protective agent of cornea, and preparation method and application of cross-linking protective agent of cornea

A technology of protective agent and cross-linking agent, which is applied in medical science, prosthetics, eye implants, etc., and can solve problems such as poor stability, calcification, and low mechanical properties

Inactive Publication Date: 2020-05-19
SHANDONG EYE INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, there were also problems of low mechanical properties, poor stability and calcification in the application of decellularized heart valves, great saphenous veins, and aortic tissues. These problems were mostly solved by introducing exogenous cross-linking agents.

Method used

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  • Cross-linking protective agent of cornea, and preparation method and application of cross-linking protective agent of cornea
  • Cross-linking protective agent of cornea, and preparation method and application of cross-linking protective agent of cornea

Examples

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preparation example Construction

[0019] The present invention also provides a preparation method of the cross-linking protective agent, comprising the following steps: (1) dissolving the cross-linking agent in PBS buffer solution to obtain a cross-linking agent solution; The mass fraction of the crosslinking agent is 0.1 to 1%;

[0020] (2) adding the protecting agent into the crosslinking agent solution to obtain a crosslinking protecting agent, and the mass fraction of the protecting agent in the crosslinking protecting agent is 5-20%.

[0021] The present invention preferably prepares solutions with different mass fractions for different types of cross-linking agents. When the cross-linking agent is proanthocyanidin, it is preferably prepared as a solution with a mass fraction of 0.5%; when the cross-linking agent is epigalloc When catechin gallate is used, it is preferably prepared as a solution with a mass fraction of 0.8%; when the crosslinking agent is genipin, it is preferably prepared as a solution w...

Embodiment 1

[0030] Proanthocyanidin (PA) and BSA were added to PBS in sequence to prepare a cross-linking protective solution containing 0.5% PA and 10% BSA in mass fraction, respectively, to carry out corneal cross-linking;

[0031] The results are: after crosslinking, visual observation of the transparency of the cornea shows that the cornea obviously becomes light brown, and the thickness of the cornea is measured with a micro-thickness gauge 1 and 2 hours after crosslinking. After cross-linking for 2 hours, the change in corneal thickness was 106.9 μm, and the corneal thickening was less. The elastic modulus of the cornea measured by an electronic stretching machine was 4.86 MPa, and the mechanical properties were significantly improved (see attached figure 1 ).

[0032] Control 1

[0033] Corneal cross-linking is carried out with a PBS solution of 0.5% proanthocyanidins by mass fraction;

[0034] The results are: After crosslinking, visual observation of the transparency of the cor...

Embodiment 2

[0036]Adding genipin (GNP) and BSA to PBS in sequence to prepare a cross-linking protective solution containing 0.5% GNP and 10% BSA in mass fractions respectively, to carry out corneal cross-linking;

[0037] The result is: after crosslinking, visual observation of the transparency of the cornea shows that the cornea obviously turns light blue, and 1 and 2 hours after crosslinking, the thickness of the cornea is measured using a micro-thickness gauge. The change in corneal thickness is 95.8 μm after 1 hour After 2 hours of cross-linking, the corneal thickness change was 144.7 μm, and the corneal thickening was less. The elastic modulus of the cornea measured by an electronic stretching machine was 5.66 MPa, and the mechanical properties were significantly improved (see appendix figure 1 ).

[0038] Control 2

[0039] Using a 0.5% genipin PBS solution to carry out corneal cross-linking;

[0040] The results are: after crosslinking, visual observation of the transparency of t...

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Abstract

The invention provides a cross-linking protective agent of a cornea, and a preparation method and application of the cross-linking protective agent of a cornea, and relates to the technical field of cross-linking protective agents. The cross-linking protective agent is prepared through adding a protective agent namely bovine serum albumin (BSA) to a natural cross-linking agent solution. The cross-linking protective agent disclosed by the invention can effectively increase the transparency of a cross-linking cornea, can protect cornea thickness changes and can effectively improve mechanical properties of the cornea.

Description

technical field [0001] The invention belongs to the technical field of cross-linking protective agents, and in particular relates to a corneal cross-linking protective agent and its preparation method and application. Background technique [0002] Corneal disease is the second most common blind eye disease in my country, and corneal transplantation is still an effective treatment. Corneal transplantation is divided into penetrating keratoplasty, lamellar keratoplasty and corneal endothelial transplantation, of which lamellar keratoplasty accounts for about 100% of the total corneal transplantation. 50%. But the current global survey shows that there is a serious shortage of corneal transplant donors, and only one cornea can be used for 70 people. To alleviate the shortage of corneal tissue, several alternatives to human corneal donors have been developed, such as artificial corneas, collagen biosynthetic implants, and cell-based replacements. However, some complications hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/00A61L27/50A61F2/14
CPCA61F2/142A61L27/00A61L27/227A61L27/50A61L2430/16C08L89/00
Inventor 谢立信周庆军史伟云李华
Owner SHANDONG EYE INST
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