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Application of acetylacetone in inhibiting growth of blue-green algae

A technology of acetylacetone and cyanobacteria, applied in the direction of application, plant growth regulator, chemicals for biological control, etc., to achieve the effect of low cost

Active Publication Date: 2020-05-19
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above data show that the application of acetylacetone in natural water bodies will not cause adverse environmental impact; although acetylacetone is widely used as a raw material for synthetic insecticides and fungicides, so far there has been no report of using it as an algastatic agent

Method used

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  • Application of acetylacetone in inhibiting growth of blue-green algae
  • Application of acetylacetone in inhibiting growth of blue-green algae
  • Application of acetylacetone in inhibiting growth of blue-green algae

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] (1) Inhibitory effect of acetylacetone on high-yielding strain FACHB-905 Microcystis aeruginosa

[0044] Add 100ml of BG-11 medium to a 150ml Erlenmeyer flask under sterile conditions, and insert algae, so that the OD 680 0.2, and add different volumes of AA stock solution diluted 100 times to the Erlenmeyer flask, so that the dosage of AA is 5mg / L, 7mg / L, 10mg / L, 15mg / L and 20mg / L, and set different Containing the control experiment (Control) of AA; Algae liquid is put into light incubator and cultivated, considering that the most suitable growth light of existing report cyanobacteria is 2000-4000LX (referring to " Chinese Academy of Sciences freshwater algae pool ", website " http: / / algae.ihb.ac.cn / Products / ProductDetail.aspx?product=523" and the literature "Effects of light on the growth and chlorophyll fluorescence of Chlorella and Microcystis aeruginosa, Journal of Xi'an University of Arts and Science, 2019, 22, 73 -77"), so in the present embodiment, the light i...

Embodiment 2

[0049] Add 100ml of BG-11 medium to a 150ml Erlenmeyer flask under sterile conditions, and insert algae, and finally get OD 680 0.11, 0.27, 0.62 and 1.0 of the algae solution, and add a certain volume of AA stock solution diluted 100 times to the Erlenmeyer flask, so that the dosage of AA is 15mg / L, and at the same time, it does not contain AA under different algae densities. The control test (Control); put the algae liquid into the light incubator for cultivation, and take samples under aseptic conditions to detect the OD at the initial stage of cultivation and after cultivation for 1d, 2d, 3d, 4d, and 5d 680 .

[0050] image 3 is the change of algae optical density within 5 days under the action of different initial concentrations of algae under the action of 15mg / L AA concentration. It can be seen from the figure that the optical density value of algae is OD 680 When changing from 0.11 to 1.0, AA showed excellent inhibitory performance on Microcystis aeruginosa, which in...

Embodiment 3

[0053] Add 300ml of BG-11 medium to a 500ml Erlenmeyer flask under sterile conditions, and insert algae, so that the OD 680 0.2, and add the concentrated stock solution of AA, anthraquinone, lysine, ethyl 2-methylacetoacetate, gallic acid, pyrogallic acid and sanguinarine to the Erlenmeyer flask, so that pyrogallic acid and The dosage of sanguinarine is 5 and 0.5mg / L respectively, and the dosage of other algastatic agents is 15mg / L. At the same time, a control experiment (Control) without algastatic agent is set up; the algae liquid is placed in the light Cultivate in an incubator, take samples under aseptic conditions to detect the content of chlorophyll a at the initial stage of cultivation and after 1d, 2d, and 3d of cultivation, and evaluate the effects of various algastatic agents by the change of chlorophyll a content.

[0054] Figure 4 It is the comparison of the inhibition rate of various algastatic agents to Microcystis aeruginosa, by Figure 4 It can be seen that ...

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Abstract

The invention provides an application of acetylacetone in inhibiting growth of blue-green algae. Acetylacetone is added into a blue-green algae growth water area, and the adding amount is not less than 7mg / L, so that the growth of the blue-green algae is controlled; acetylacetone acts on a photosynthetic system to control the growth of algae, the effect of acetylacetone is superior to that of a common algistat (such as pyrogallic acid, gallic acid and anthraquinone), acetylacetone is harmless to bacteria including four common bacteria including escherichia coli, salmonella, bacillus subtilis and staphylococcus aureus, and acetylacetone has environmental friendliness and is easy to apply and popularize.

Description

technical field [0001] The invention relates to the field of algae inhibitors, in particular to the application of acetylacetone in inhibiting the growth of cyanobacteria in eutrophic water bodies. Background technique [0002] In recent years, cyanobacteria blooms caused by eutrophication of water bodies have seriously affected people's lives. Cyanobacteria blooms will not only cause the death of other aquatic organisms and change the aquatic ecological environment, but also directly affect the safety of human water supply. Cyanobacteria are generally divided into drug-producing strains (such as FACHB-905) and non-drug-producing strains (FACHB-469), wherein the drug-producing strains release microcystins that are toxic to the liver. Microcystis aeruginosa is the most typical algae during water blooms, and it is also the most common toxic cyanobacteria. It has strong viability and is difficult to remove. Therefore, it is of great significance to effectively control the grow...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N35/02A01P13/00
CPCA01N35/02C02F1/50C02F2103/007C02F2303/20Y02W10/37A01P13/00A01N25/02
Inventor 张淑娟金吉媛王晓萌王鑫张文涛
Owner NANJING UNIV
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