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Monascus strain, fermented soybean meal fermented by monascus strain and aquatic functional biological feed containing monascus strain

A technology of fermenting soybean meal and monascus, applied in animal feed, animal feed, microorganisms, etc., to achieve the effects of promoting growth, enhancing immunity, and bright body color

Active Publication Date: 2020-05-12
福建大北农华有水产科技集团有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of monascus fermented products in livestock and poultry is mainly used to improve the quality of poultry eggs, increase the color of egg yolk, improve the color of muscles, and reduce cholesterol, etc., but there is no relevant report in the field of aquatic products

Method used

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  • Monascus strain, fermented soybean meal fermented by monascus strain and aquatic functional biological feed containing monascus strain
  • Monascus strain, fermented soybean meal fermented by monascus strain and aquatic functional biological feed containing monascus strain
  • Monascus strain, fermented soybean meal fermented by monascus strain and aquatic functional biological feed containing monascus strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The screening of embodiment 1 monascus

[0018] Source of the strain: It is taken from the Gutian area of ​​Fujian Province, and it is the starter (five kinds) used by the residents to make red distilled acid.

[0019] Isolation of Monascus:

[0020] Take two grains of different red yeast rice and crush them into powders, spread them on PDA medium with a tripod, incubate them at 30°C for 3-4 days, pick and shake the growing Monascus in sterile water test tubes, and further purify them by dilution plate method . The purified spore liquid of Monascus was added into a centrifuge tube containing sterilized glycerin and stored at -80°C.

[0021] Liquid fermented Monascus:

[0022] Add 10% Monascus spore liquid into 100mL liquid medium and cultivate at 30°C and 180r / min for 10 days. The liquid medium formula is: glucose 10%, peptone 1%, sodium nitrate 0.2%, potassium dihydrogen phosphate 0.2%, seven Magnesium sulfate water 0.05%, calcium chloride 0.01%.

[0023] The five...

Embodiment 2

[0039] Embodiment 2 monascus seed liquid culture

[0040] Optimization of seed liquid culture temperature:

[0041] Take the slant of the preserved Monascus test tube and put it into 5 500mL Erlenmeyer flasks containing 100mL liquid seed medium, and place them in culture at 26°C, 28°C, 30°C, 32°C and 34°C at a speed of 250r / min. Cultivate for 3 days in the shaker of the same method, measure the influence of different culture temperatures on the output of monascus pigment, the formula of liquid seed culture medium is: cornstarch 3.0%, cottonseed protein 1.0%, K 2 HPO 4 ·3H 2 O 1.0%, ZnSO 4 ·7H 2 O 1.0%, MgSO 4 ·7H 2 O 0.5%, pH 7.0.

[0042] Determination method of seed liquid color value:

[0043] Take 1 mL of the seed liquid and add it to a test tube containing 9 mL of 75% ethanol solution (pH 6.0-7.0) and shake well, centrifuge after 3 hours to take the supernatant, use 75% ethanol solution as a blank control, and measure light at 510nm and 410nm wavelengths. Absorpt...

Embodiment 3

[0050] The cultivation of embodiment 3 soybean meal monascus fermented product

[0051] Inoculate the Monascus seed solution prepared in Example 1 on the sterilized soybean meal medium with a water content of 35% to 40% according to the inoculum amount of 20%, mix it evenly, spread it flat in a shallow dish, and place it in a constant temperature room at 30°C for cultivation For 7 days, the humidity was controlled to be 40%. After the solid-state fermentation was completed, it was dried by filter bed drying. The fermented material is pulverized to 60 mesh with a pulverizer. The finished product of fermented soybean meal is routinely tested, and the testing methods and indicators are as follows:

[0052] Crude protein detection method: refer to GB / T 6432-2018 crude protein detection method in feed.

[0053] Crude fiber detection method: refer to GB / T 6434-2006 crude fiber detection method in feed.

[0054] Moisture detection method: refer to GB / T 6435-2014 Determination of moi...

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Abstract

The invention belongs to the field of biological feed, and relates to a monascus strain, biological feed fermented by the monascus strain and application of the red yeast rice biological feed in aquaculture. The monascus strain can generate multiple kinds of functional metabolites of monascus pigment, protease, amylase, lipase, ergosterol, statins, gamma-aminobutyric acid and the like, and fermentation products contain small peptides. By applying the biological feed to aquaculture, the survival rate and immune functions of aquatic animals can be significantly improved, and the growth performance is improved.

Description

technical field [0001] The invention belongs to the field of biological feed, and relates to a monascus strain and biological feed prepared by fermentation thereof, and the application of the red yeast biological feed in aquaculture. Background technique [0002] Monascus fungi (Eumycophyta), Ascomycetes (Ascomycetes), Euascomycetes (Euascomycetes), Monascus (Monascus). Monascus is a saprophytic fungus, the optimum pH for growth is 3.5-5, it can tolerate pH 3.5, and it is especially fond of lactic acid. The growth temperature is 26-42°C, the optimum temperature is 32°C-35°C, and it can tolerate 10% ethanol. Monascus has attracted attention for its ability to produce a large amount of natural red pigment. [0003] Monascus has a long history of production and use in my country, has high medicinal value, and can also be used for wine making and vinegar making. Monascus can produce a variety of enzymes, mainly including amylase, protease, esterase and cellulase. At present,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A23K10/12A23K10/18A23K10/37A23K50/80C12R1/645
CPCA23K10/12A23K10/18A23K10/37A23K50/80C12R2001/645C12N1/145Y02P60/87
Inventor 陈淑琼汪攀易敢峰孙云章王云爽李栋李振江贠桂玲李曼
Owner 福建大北农华有水产科技集团有限公司
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