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Cell suspension for repairing striae gravidarum and preparation method of cell suspension for repairing striae gravidarum

A technology of suspension and stretch marks, applied in the field of cell culture, can solve the problems of inability to achieve the ideal treatment state, complicated postoperative care, irreversible damage, etc., to improve the skin defense ability, no immune rejection, and promote the growth of connective tissue. Effect

Inactive Publication Date: 2020-05-08
JINAN PANSHENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the medical aesthetics method has certain risks, and the postoperative care is also relatively complicated. If it is not handled properly, it may cause some irreversible damage, and the recovery time is relatively long; although external creams are more convenient and safe to use, their effects are limited and cannot achieve ideal treatment status

Method used

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  • Cell suspension for repairing striae gravidarum and preparation method of cell suspension for repairing striae gravidarum
  • Cell suspension for repairing striae gravidarum and preparation method of cell suspension for repairing striae gravidarum
  • Cell suspension for repairing striae gravidarum and preparation method of cell suspension for repairing striae gravidarum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of embodiment 1 cell suspension

[0032] 1. Experimental materials

[0033] Penicillin-streptomycin, type Ⅰ collagenase, dispase, trypsin, DMEM medium, F12 medium, dermal fibroblast medium, M254 medium (gibco company), PBS, fetal bovine serum FBS, tissue preservation solution , T75 cell culture flask, 6-well cell culture plate, 100μm cell strainer.

[0034] 2. Experimental method

[0035] (1) Obtaining tissue: fully communicate with patients, inform the purpose, method and expected treatment effect of this project, and inform them of possible risks and sign an informed consent. A 2cm*2cm piece of tissue is excised from the body without stretch marks, then placed in a container containing tissue preservation solution, and transported to the cell culture laboratory.

[0036] (2) Tissue treatment: cut off the fat and connective tissue in the subcutaneous tissue attached to the skin tissue, disinfect the collected fresh tissue samples with alcohol for 3 m...

Embodiment 2

[0047] Embodiment 2 cell suspension animal experiment treatment evaluation

[0048] 1. Animals and equipment

[0049] 1. Experimental animals: healthy 10-week-old nude mice, male, weighing about 20 g.

[0050] 2. Reagents and equipment

[0051] Skin Hydroxyproline Determination Kit, Suspension 1 (Suspension of epidermal cells and melanocytes: cell concentration is 5.0*10 6 / ml, generation is P3), suspension II (dermal fibroblast suspension: the number of dermal fibroblasts is 2.0*10 7 / ml, passage is P4), suspension three (cell suspension for injection: containing 5.0*10 6 / ml passage is P3 epidermal cells and melanocytes, containing 2.0*10 7 / ml generation is P4 dermal fibroblasts), suspension four (F12 medium), commercially available cream that claims stretch marks removal, spectrophotometer.

[0052] 2. Experimental method

[0053] (1) Treatment of experimental animals: 15 nude mice were selected and divided into five experimental groups, with 3 animals in each group....

Embodiment 3

[0063] Embodiment 3 cell suspension clinical treatment evaluation

[0064] 1. Experimental objects and reagents

[0065] 1. Subjects: 50 women aged 25-45 who have had stretch marks for more than a year and other physical indicators are healthy

[0066] 2. Reagents

[0067] Suspension 1: Suspension of autologous epidermal cells and melanocytes, the cell concentration is 5.0*10 6 / ml, the generation is P3

[0068] Suspension 2: dermal fibroblast suspension, the number of dermal fibroblasts is 2.0*10 7 / ml, the generation is P4

[0069] Suspension 3: cell suspension for injection, containing 5.0*10 6 / ml passage is P3 epidermal cells and melanocytes, containing 2.0*10 7 / ml Passage P4 dermal fibroblasts

[0070] Suspension four: F12 medium

[0071] Creams marketed that claim to remove stretch marks

[0072] 2. Experimental steps

[0073] 1. Experimental design:

[0074] 50 women aged 25-45 who had stretch marks for more than one year and other physical indicators were ...

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Abstract

The invention discloses a cell suspension for repairing striae gravidarum and a preparation method of the cell suspension for repairing striae gravidarum. By a cell culture method, a composite cell suspension containing epidermic cells capable of promoting epidermis regeneration, melanocyte capable of promoting skin color improvement, and dermal fibroblasts having effects of promoting collagen generation, supplementing fibronectin and promoting elastin synthesis, is prepared. According to the suspension, through synergistic interaction effects generated by the epidermic cells, the melanocyte and the dermal fibroblasts, inflammatory reactions are reduced, skin is compact, skin ageing is delayed, the defense capability of the skin is increased, and the striae gravidarum of the skin can be effectively repaired.

Description

technical field [0001] The invention relates to a cell suspension for repairing stretch marks and a preparation method thereof, belonging to the field of cell culture. Background technique [0002] Stretch marks are a type of stretch marks that occur during pregnancy as atrophic, streaky skin changes on the chest, abdomen, buttocks, and thighs. Early on, it appears as dark red or purple streaks, then depigmentation, atrophy, and finally a white or silvery skin lesion that stabilizes. The reason for the formation of stretch marks is that pregnancy is affected by hormones during pregnancy. Due to the swelling of the uterus and abdomen, the elastic fibers and collagen fibers of the skin are damaged or broken to varying degrees due to external force, and the skin becomes thinner and thinner. [0003] The skin can be divided into three layers from the outside to the inside, namely the epidermis, dermis and subcutaneous tissue. The dermis is located in the superficial layer of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077C12N5/071A61K8/99A61Q19/00A61Q19/08
CPCC12N5/0626C12N5/0656C12N5/0625A61K8/99A61Q19/00A61Q19/08C12N2502/00C12N2501/734A61K2800/91
Inventor 张平邢志青王红丽张甜甜李霄
Owner JINAN PANSHENG BIOTECH
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