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Undaria pinnatifida extract polysaccharides and applications thereof in preparation of alpha-glucosidase activity inhibition drugs

A technology of undaria pinnatifida and undaria pinnatifida, which is applied in the application field of preparing α-glucosidase activity inhibitory drugs, and achieves the effects of easy operation, strong inhibitory effect, and low process cost

Active Publication Date: 2020-05-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the application of sulfated polysaccharides derived from wakame in the preparation of hypoglycemic active drugs has not been reported.

Method used

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  • Undaria pinnatifida extract polysaccharides and applications thereof in preparation of alpha-glucosidase activity inhibition drugs
  • Undaria pinnatifida extract polysaccharides and applications thereof in preparation of alpha-glucosidase activity inhibition drugs
  • Undaria pinnatifida extract polysaccharides and applications thereof in preparation of alpha-glucosidase activity inhibition drugs

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Microwave-assisted water extraction of sulfated polysaccharides

[0037] 1. Polysaccharide extraction

[0038] (1) Pulverize undaria pinnatifida with a mass water content of 3%-4%, and pass through an 80-mesh sieve to obtain undaria pinnatifida powder. Then take wakame powder 130g, add in 5L round bottom flask, add 3.9L volume concentration 95% ethanol aqueous solution according to material-liquid ratio 1:30 (g / ml), after shaking and mixing, make it boil and condense and reflux for 3 hours , filtered after cooling, retained the filter residue, and dried the filter residue at 65° C. to obtain 110.4 g of defatted wakame powder, which was set aside.

[0039] (2) Then weigh 20g of degreased wakame powder, add it to a 1L microwave reaction bottle, add 0.6L of deionized water according to the ratio of solid to liquid 1:30 (g / ml), mix it and let it stand for 30min, and put it in the microwave Microwave extraction was carried out in a reaction extraction apparatus,...

Embodiment 2

[0070] Example 2: Inhibition of α-glucosidase activity by sulfated polysaccharide Up-3 and sulfated polysaccharide Up-4

[0071] 10μL, 0.15U / ml of α-glucosidase, 10μL of various concentrations (0.0001, 0.001, 0.01, 0.03, 0.05, 0.08, 0.1, 0.3, 0.5, 1.0, 2.0, 3.0, 5.0mg / ml) Polysaccharide or acarbose aqueous solution, 80 μL phosphate buffered saline solution (0.02mol / L, pH 6.8) and 10 μL 5 mM p-nitrophenyl-α-D-glucopyranoside (PNPG) aqueous solution were mixed in a 96-well plate The reaction was incubated at 37°C for 30 minutes, and the absorbance value was detected at 340 nm using a microplate reader, which was recorded as A2.

[0072] Separately add 10 μL 0.15U / ml α-glucosidase, 80 μL phosphate buffered saline (0.02mol / L, pH6.8) and 10 μL 5mM p-nitrophenyl-α-D-glucopyranoside (PNPG) The aqueous solution was mixed and reacted in a 96-well plate, incubated at 37°C for 30 minutes, and the absorbance value was detected at 340 nm using a microplate reader, which was recorded as A1...

Embodiment 3

[0081] Example 3: Hypoglycemic activity of sulfated polysaccharides Up-3 and Up-4 after meals

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Abstract

The invention discloses Undaria pinnatifida polysaccharides and applications of the Undaria pinnatifida polysaccharides in the preparation of alpha-glucosidase activity inhibition drugs. The characteristic molecular weights of the two polysaccharides Up-3 and Up-4 are 78-282 kDa and 41-314 kDa respectively; the Up-3 is mainly composed of glucuronic acid, mannose and fucose, and the Up-4 is mainlycomposed of rhamnose, glucuronic acid and fucose; and the IC50 values of the alpha-glucosidase inhibitory activity of the Up-3 and Up-4 are 113.40 + / -3.02 [mu]g / mL and 50.53 + / -3.47 [mu]g / mL respectively, and the Up-3 and Up-4 have the effect of remarkably inhibiting blood glucose rise caused by oral maltose. Therefore, the sulfated polysaccharides Up-3 and Up-4 can reduce the postprandial blood sugar level by inhibiting the activity of alpha-glucosidase in intestinal tracts so as to achieve the effect of reducing blood sugar.

Description

technical field [0001] The invention relates to two sulfated polysaccharides derived from wakame seaweed and the application thereof in the preparation of α-glucosidase activity inhibiting drugs. Background technique [0002] Diabetes is a chronic metabolic disorder with high blood sugar caused by insulin resistance or insufficient insulin secretion. In recent years, the number of people with diabetes in the world has increased year by year. The number of people with diabetes in the world has reached 425 million and this number is estimated to reach 462 million in 2040 (IDF Diabetes Atlas), of which WHO (2018) reported that about 90% of diabetic patients were diagnosed with type 2 diabetes. Type 2 diabetes is mainly characterized by defects in insulin secretion and insulin resistance. In the early stage of the onset, hyperglycemia is finally caused by increased hepatic glucose production and decreased peripheral glucose uptake. [0003] α-glucosidase is an important digesti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/737A61P3/10A23L33/125
CPCC08B37/0003C08B37/0063A61K31/737A61P3/10A23L33/125A23V2002/00A23V2200/328A23V2250/51A23V2250/21
Inventor 王鸿钟启武周涛顺邱文辉魏斌岳少鹏
Owner ZHEJIANG UNIV OF TECH
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