Cultivation method for wild ganoderma lucidum karst
A cultivation method and technology of Ganoderma lucidum, applied in the field of asexual reproduction of wild Ganoderma lucidum, can solve the problems that the cultivation technology of Ganoderma lucidum cannot meet the needs of large-scale production, the survival rate of strain propagation is not high, and the quality of strains is poor, so as to achieve large-scale production, Low cost, facilitated formation effect
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Embodiment 1
[0026] (1) Acquisition of Ganoderma lucidum entities: In early August 2016, wild Ganoderma lucidum with good growth, no pests and diseases, and good color was collected as explants. Rinse 2-3 times, then wipe the Ganoderma lucidum with absorbent cotton dipped in a 1.5% volume concentration of detergent solution and set aside;
[0027] (2) Preparation of female species: with potato 100g, rice milk 10g, sugar 10g, agar 10g, water 50g as female seed culture medium, described rice milk is the product that rice and water beating obtain, and described rice and water weight ratio Put it into a test tube at a ratio of 1:2, put it into a test tube at a pressure of 103.4kPa and a temperature of 121.3°C, autoclave it for 15-20 minutes to prepare a potato agar test tube for use, and inoculate the collected wild Ganoderma lucidum fruiting bodies by tissue separation Put it on the slant of the potato agar test tube, and cultivate it at a constant temperature of 25°C for 13 days to grow snow...
Embodiment 2
[0032] (1) Acquisition of Ganoderma lucidum entities: In mid-August 2016, wild Ganoderma lucidum with good growth, no pests and diseases, and good color was collected as explants. Rinse with tap water 2-3 times, then wipe the ganoderma lucidum with absorbent cotton dipped in a 1.5% volume concentration of detergent solution, and set aside;
[0033] (2) Preparation of female species: with potato 200g, rice milk 20g, sugar 20g, agar 20g, water 100g as female seed culture medium, described rice milk is the product that rice and water beating obtain, and described rice and water weight ratio Put it into a test tube at a ratio of 1:3 and put it into a test tube at a pressure of 103.4kPa and a temperature of 121.3°C. After autoclaving for 18 minutes, a potato agar test tube was prepared for use. The collected wild Ganoderma lucidum fruiting bodies were inoculated into potatoes by tissue separation. The slant of the agar test tube can grow snow-white mycelium at a constant temperatur...
Embodiment 3
[0038] (1) Acquisition of Ganoderma lucidum entities: In late August 2016, wild Ganoderma lucidum with good growth, no pests and diseases, and good color was collected as explants. Rinse with tap water 2-3 times, then wipe the ganoderma lucidum with absorbent cotton dipped in a 1.5% volume concentration of detergent solution, and set aside;
[0039] (2) Preparation of female species: with potato 300g, rice milk 30g, sugar 30g, agar 30g, water 200g as female seed culture medium, described rice milk is the product that rice and water beating obtain, described rice and water weight ratio Put it into a test tube at a pressure of 103.4kPa and a temperature of 121.3°C at a ratio of 1:5, and autoclave for 20 minutes to prepare a potato agar test tube for use. The collected wild Ganoderma lucidum fruiting bodies are inoculated into potatoes by tissue separation. The slant of the agar test tube can grow snow-white mycelium at a constant temperature of 28°C for 18 days, and select high-...
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