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Biochar-based immobilized polycyclic aromatic hydrocarbons high-efficiency degradation bacterial agent and its preparation method and application

A technology for efficiently degrading bacteria and polycyclic aromatic hydrocarbons, applied in biochemical equipment and methods, microorganism-based methods, immobilized on or in inorganic carriers, etc., can solve the problems of low degradation rate of polycyclic aromatic hydrocarbons, and achieve high degradation. ability, prolonging the survival time, the effect of high degradation rate

Active Publication Date: 2021-03-02
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] The purpose of the present invention is to provide a biochar-based immobilized polycyclic aromatic hydrocarbon high-efficiency degradation bacterial agent and its preparation method and application, so as to obtain a biochar-based fixed polycyclic aromatic hydrocarbon efficient degradation bacterial agent with good activity and good degradation effect, and then solve the problem of The problem of low degradation rate of polycyclic aromatic hydrocarbons in the prior art

Method used

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  • Biochar-based immobilized polycyclic aromatic hydrocarbons high-efficiency degradation bacterial agent and its preparation method and application
  • Biochar-based immobilized polycyclic aromatic hydrocarbons high-efficiency degradation bacterial agent and its preparation method and application
  • Biochar-based immobilized polycyclic aromatic hydrocarbons high-efficiency degradation bacterial agent and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0054] Preparation of biochar-based immobilized microbial agent

[0055] Use an inoculation loop to pick a strain from the LB plate of AD-3 (Martelella sp.AD-3) and inoculate it in 5mL of nutrient solution. The composition of nutrient solution (g / L) is: peptone 8.0- 12.0; yeast powder 4.0-6.0; NaCl 8-12, activate for 36 hours at 30°C in a shaking incubator with a rotation speed of 150r / min;

[0056] Take 5m of the activated bacterial liquid into 200mL of 3% LB to expand the culture, add 50mg / L of phenanthrene to provide a certain selection pressure of PAHs, at 30°C, rotate at 150r / min, cultivate for 48h, take out the Erlenmeyer flask, and wash the bacteria repeatedly Body 3 times.

[0057] According to the volume ratio of 10% inoculum, take the expanded culture and add it to the conical flask with 20mL 3% LB. Centrifuge on a high-speed centrifuge at 8000r / min, remove the supernatant, and obtain 1# bacterial agent. Scanning electron microscope images of cotton stalk biochar-...

Embodiment 2

[0061] Optimization of biochar-based immobilized microbial agent immobilized medium

[0062] According to the method of Example 1, the influence of different solidification medium on obtaining bacterial agent was investigated.

[0063] Among them, 1# bacterial agent means that it is not fixed on the biochar-based material, 2# bacterial agent means it is fixed on the cotton stalk biochar-based material, and 3# bacterial agent means it is fixed on the rice husk biochar-based material.

[0064] Select 1# solidified medium, 2# solidified medium, and 3# solidified medium to prepare cotton stalk and rice husk biochar-immobilized microbial bacterial agents, that is, weigh 1.0 g of cotton stalk biochar or rice husk biochar in Put the 100mL Erlenmeyer flask at 121°C for 20min under high temperature and high pressure sterilization, cool to room temperature, add 20mL of 1# solidified medium, 2# solidified medium or 3# solidified medium that has resuspended bacteria, and place at 30 ℃, f...

Embodiment 3

[0071] Optimization of curing time of biochar-based immobilized microbial agent

[0072] According to the method of Example 1, the influence of different curing times on obtaining bacterial agents was investigated.

[0073] Among them, 1# bacterial agent means that it is not fixed on the biochar-based material, 2# bacterial agent means it is fixed on the cotton stalk biochar-based material, and 3# bacterial agent means it is fixed on the rice husk biochar-based material.

[0074] The solidification time is 1d, 2d, and 3d respectively to prepare cotton stalk and rice husk biochar to solidify microbial agents, that is, weigh 1.0g of cotton stalk biochar or rice husk biochar and put it in a 100mL Erlenmeyer flask at a high temperature of 121°C Autoclave for 20 minutes, cool to room temperature, add 20mL of 2# solidified medium that has resuspended bacteria, place in a constant temperature shaking incubator at 30°C with a rotation speed of 100r / min, fix for 1d, 2d, and 3d respecti...

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Abstract

The invention relates to a biochar-based fixed polycyclic aromatic hydrocarbon efficient degradation bacterial agent, which includes a biochar-based material and a bacterial strain adsorbed on the biochar-based material. The biochar-solidified bacterial agent is used for degrading polycyclic aromatic hydrocarbons. The present invention prepares biochar-cured bacterial agents with the largest amount of attached microorganisms and the best removal effect by optimizing the solidification medium and curing time. The removal rates of these two bacterial agents for phenanthrene can reach 93.08% and 66.36% respectively in 16 hours; Since the free bacteria have basically lost their activity after 7 days at room temperature, the biochar-solidified bacterial agent still has a good removal effect, and the removal rates of phenanthrene can still reach 71.78% and 40.72%, respectively. It is obvious that biochar can maintain the activity of the bacteria , Prolong the survival time of microorganisms.

Description

technical field [0001] The invention belongs to the technical field of microbial bacterial agents, and in particular relates to a biochar-based fixed polycyclic aromatic hydrocarbon efficient degradation bacterial agent and a preparation method and application thereof. Background technique [0002] Because oil extraction will cause very serious polycyclic aromatic hydrocarbons (PAHs) pollution to the soil around the oil field, PAHs is a typical persistent organic pollutant in the soil, and its toxicity and carcinogenicity are extremely harmful to the environment, so it has been approved by the US Environmental Protection Agency and the European Union. listed as priority pollutants. Therefore, it has become one of the hot spots of environmental protection to explore how to efficiently and economically treat PAHs under high-salt conditions. [0003] At present, the use of microorganisms to degrade soil PAHs is considered to be the best way to bioremediate soil PAHs pollution....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N11/14B09C1/10C12R1/01
CPCB09C1/10C12N1/20C12N11/14
Inventor 崔长征任静沈佳敏张磊陈欣林匡飞刘勇弟
Owner EAST CHINA UNIV OF SCI & TECH
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