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Endophytic fungus strain capable of enhancing activity of acid phosphatase of casuarina equisetifolia in low-phosphorus-content environment

A technology of endophytic fungi and casuarina, applied in the field of microorganisms to achieve the effect of enhancing acid phosphatase activity and promoting plant growth

Active Publication Date: 2020-05-01
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Endophytic fungi can enhance plant physiological characteristics under adversity stress, but there are few reports on endophytic fungi enhancing the stress resistance of woody host plants

Method used

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  • Endophytic fungus strain capable of enhancing activity of acid phosphatase of casuarina equisetifolia in low-phosphorus-content environment
  • Endophytic fungus strain capable of enhancing activity of acid phosphatase of casuarina equisetifolia in low-phosphorus-content environment
  • Endophytic fungus strain capable of enhancing activity of acid phosphatase of casuarina equisetifolia in low-phosphorus-content environment

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Isolation of endophytic fungi from casuarina

[0013] 1. Main equipment

[0014] Ultra-clean workbench SW-CJ-1FD, constant temperature incubator HH•B11-II, constant temperature culture oscillator zhwy-211B, ten-thousandth balance AR1140, automatic vertical sterilizer LMQ.C-4060, ultrapure water Machine P60-CW etc.

[0015] 2. Main Reagents and Media

[0016] Reagents: 15% sodium hypochlorite, 75% ethanol, primer PAGE 11-59bp OD 1-2, DNA electrophoresis loading buffer, GoodViewTM nucleic acid dye, 2×Tap PCR MasterMix, fungal DNA extraction kit, DNA purification and recovery kit.

[0017] Medium: (1) Modified Martin agar medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o 6 •H 2 O) 20.0g, Potassium dihydrogen phosphate (KH 2 PO 4 ) 1.0 g, magnesium sulfate (MgSO 4 •7H 2 O) 0.5g, agar 15.0g, ultrapure water 1000ml, pH 6.2~6.6.

[0018] (2) Improved Martin's liquid medium: peptone 5.0g, yeast extract powder 2.0g, glucose (C 6 h 12 o ...

Embodiment 2

[0039] Bacterial solution preparation: The screened endophytic fungi (G3) were cultured in potato dextrose agar medium at 28°C for one week, then inserted into 60 mL potato dextrose liquid medium, and cultured in a constant temperature shaker for 72 h (28°C, 160 r min -1 ), calculate the number of spores by hemocytometer method, and dilute the cultured bacterial solution with sterile water to 5.5×10 6 pcs L -1 . After that, it was diluted with 40 mL of sterile water, and this concentration and dosage were used for inoculation in subsequent experiments.

[0040] This experiment adopts the soil culture pot experiment, and selects the annual short-branch casuarina from the same mother tree to carry out the soil culture pot experiment of seedlings. The seedlings are provided by the Chihu Shelter Forest Farm in Huian County, Fujian Province. The potting soil is yellow heart soil and sandy soil 3:1 Proportional mixing (see Table 1 for soil nutrient content), formaldehyde disinfec...

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Abstract

The invention discloses endophytic fungus strain capable of enhancing activity of acid phosphatase of casuarina equisetifolia in low-phosphorus-content environment. The endophytic fungus strain is named Herpotrichia australis G3, and is registered and preserved in China General Microbiological Culture Collection Center since November 20, 2019 under the preservation number of CGMCC No. 18816. The endophytic fungus strain is separated and purified from the root of the casuarina equisetifolia, and is capable of significantly improving the activity of the acid phosphatase of the casuarina equisetifolia so as to enhance adaptability of seedlings in the low-phosphorus-content environment.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to an endophytic fungus capable of enhancing the activity of casuarina acid phosphatase in a low-phosphorus environment. Background technique [0002] Acid phosphatase is an induced enzyme and hydrolase widely distributed in different tissues of plants. It releases inorganic phosphorus by degrading organic phosphorus compounds, and promotes the absorption, activation and reuse of phosphorus in plants. Synthesis of phosphatase inside and outside plant cells in phosphorus-deficient environment. The phosphatase secreted outside the cell will promote the activation of organic acids and improve the availability of phosphorus in the soil. The acid phosphatase secreted by plant roots can hydrolyze phospholipids in the soil, making the soil insoluble Phosphorus is activated for uptake and utilization by plants. Therefore, the acid phosphatase synthesized and secreted by plant leav...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01C11/02A01N63/30A01P21/00
CPCA01G7/06A01C11/02
Inventor 梁安洁李键吴承祯洪滔林勇明
Owner FUJIAN AGRI & FORESTRY UNIV
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