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Beautifying and anti-aging composition and application thereof

A composition and anti-aging technology, applied in the directions of medical preparations containing active ingredients, cosmetic preparations, preparations for skin care, etc. Effect

Pending Publication Date: 2020-04-17
孙建军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This makes the application of some molecules with therapeutic value but no cell membrane penetration in the fields of cell biology and medicine greatly limited

Method used

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  • Beautifying and anti-aging composition and application thereof
  • Beautifying and anti-aging composition and application thereof
  • Beautifying and anti-aging composition and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0019] The preparation method of the nucleated cells is not particularly limited in the present invention, preferably using the kit and method in the Chinese patent CN200610106875.5 (an in vitro separation kit for nucleated cells and its application method) to carry out the nucleated cells in autologous blood Extraction of nuclear cells. Preferably, the nucleated cells in the present invention also include stem cells, platelets, cytokines and plasma proteins. The nucleated cells of the present invention are rich in TAT protein, which can carry macromolecular substances into the cells, and can mediate exogenous proteins, nucleic acids and other substances into the cells safely and effectively under the condition that the cell membrane is not damaged.

[0020] The volume ratio of water for injection in the present invention to nucleated cells is preferably 4:1, and the volume of nucleated cells in the present invention is preferably the volume of the solution obtained by direct ...

Embodiment 1

[0027] Blood collection: 25 mL of autologous peripheral blood was collected;

[0028] Separation and extraction: Use the nucleated cell in vitro separation kit to separate and extract the cell material, and purify the nucleated cells containing TAT protein;

[0029] Injection: Using microneedles, the purified autologous nucleated cells combined with medical sterile injection water are directly delivered to the part to be improved, each injection is 5mL, and the effect of calming neck lines is achieved after 4 times. The before and after comparison is as follows figure 1 shown.

Embodiment 2

[0031] According to the same method as in Example 1, the injection volume is 2.5mL / time, and the effect of calming crow's feet is achieved after 3 times. The comparison before and after is as follows figure 2 shown.

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Abstract

The invention, which relates to the technical field of beautifying products, provides a beautifying and anti-aging composition and application thereof. The separated and extracted karyocyte is rich inTAT protein belonging to cationoid type cell transmembrane peptide and foreign protein, nucleic acid and other substances can be mediated to safely and effectively enter the cell under the conditionof not destroying the cell membrane; the karyocyte is directly conveyed to the subcutaneous part, minimally invasive surfaces can be quantitatively, accurately and finely formed on epidermis, dermis and subcutaneous surfaces in a fixed-point and fixed-layer manner; the minimally invasive surfaces start self-healing and generate collagenous fibers and viscous protein, the collagenous fibers and theviscous protein serve as adhesives between cells, and the karyocyte is combined to activate cell regeneration functions at different levels, so that the effects of wrinkle smoothing, plumping, loosening, tightening and prolapse improvement can be realized. Meanwhile, the subcutaneous thickness can be continuously increased, wrinkles can be smoothened, sunken parts can be accurately filled, and the effect of skin plumpness is realized.

Description

technical field [0001] The invention belongs to the technical field of beauty products, and in particular relates to a beauty and anti-aging composition and its application. Background technique [0002] Human skin is just like the annual rings of a big tree. As time goes by, the annual rings of the tree will become wider and wider, and the distance between human wrinkles will become larger and larger, and the skin will no longer be firm. This is because the skin It is caused by the aging of cell tissue and the loss of collagen. The current treatment methods used by medical aesthetic institutions and doctors, such as injection micro-plastic surgery, photoelectric equipment, and thread carving, etc., are mostly physical lifting, which is superficial. [0003] Cell therapy and cytokine therapy are at the heart of regenerative medicine. Autologous stem cells in blood have powerful replication and repair functions. Autologous stem cells contain a high concentration of regener...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61Q19/08
CPCA61K8/99A61Q19/08A61K2800/91
Inventor 孙建军
Owner 孙建军
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