Edible fungus polysaccharide extraction method, edible fungus polysaccharide and edible fungus beverage
A polysaccharide extraction and edible fungus technology is applied in the fields of edible fungus polysaccharide, edible fungus polysaccharide extraction method, and edible fungus beverages, which can solve the problem of low polysaccharide extraction rate, improve extraction efficiency and extraction quantity, prolong shelf life, and facilitate fermentation and reproduction. Effect
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[0119] The preparation process of the second agar medium includes: mixing the fifth edible fungus powder and water and boiling, filtering through at least 3 layers of gauze, adding agar powder and the fourth sugar source, heating to 75-85°C, supplementing the preparation In the process of losing water, put the inclined plane to obtain the second agar medium.
[0120] The fifth edible fungus powder has a mesh size ≥ 300, and the fifth edible fungus includes but is not limited to at least one of the following: shiitake mushroom, Hericium erinaceus, black fungus, ganoderma lucidum, Phellinus japonica, white fungus, Inonotus obliquus, Morels.
[0121] The fourth sugar source can be monosaccharide or polysaccharide, including but not limited to at least one of the following: glucose, brown sugar, and fructose.
[0122] Back inoculation culture In order to further purify the domesticated strains of solid culture, select the strains adapted to the purification. 23 to 25 degrees Cel...
Embodiment 1
[0155] This embodiment provides a method for extracting polysaccharides from edible fungi, including:
[0156] S1011, making the fruiting bodies of mushrooms into powder, and passing through a 300-mesh sieve to obtain mushroom powder;
[0157] S1021. Mix 15g of glucose, 1000mL of water and 20g of shiitake mushroom powder in S1011, and perform one stirring, and then carry out one sterilization treatment and one cooling in turn to obtain the first culture medium; The stirring time is 150r / min, and the stirring time is 8 minutes; in the first sterilization treatment, the sterilization pressure is 0.15Pa, the sterilization temperature is 125°C, and the sterilization time is 0.5h; in the first cooling, the cooling rate is 4 ℃ / min, cooling to a temperature ≤ 25 ℃;
[0158] S1031. Perform primary selection, solid medium acclimatization, backgraft culture, and liquid medium acclimatization of the mushroom strain in sequence to obtain the first strain;
[0159] The primaries are:
...
Embodiment 2
[0178] This embodiment provides a method for extracting polysaccharides from edible fungi, including:
[0179] S1011, making black fungus fruiting bodies into powder, passing through a 300-mesh sieve to obtain black fungus powder;
[0180] S1021, mixing 15g of glucose, 1000mL of water and 20g of black fungus powder in S1011, and stirring once, followed by a sterilization treatment and a cooling after the first stirring, to obtain the first culture medium; in the first stirring, the stirring speed is 150r / min, stirring time is 8 minutes; in the first sterilization treatment, the sterilization pressure is 0.15Pa, the sterilization temperature is 125°C, and the sterilization time is 0.5h; in the first cooling, the cooling rate is 4°C / min, cooling to a temperature ≤25°C;
[0181] S1031. Perform primary selection, solid medium acclimatization, backgraft culture, and liquid medium acclimatization of black fungus strains in sequence to obtain the first strain;
[0182] The primari...
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