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Electrochemiluminescence sensor for detecting insulin by quenching CdS hybridized TiO2 nanoribbon through gold-curcumin nanoparticles

A nanoparticle, curcumin technology, applied in the direction of material electrochemical variables, scientific instruments, instruments, etc., to achieve the effect of short response time, high sensitivity detection, and low dosage

Active Publication Date: 2020-03-24
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently based on gold-curcumin nanoparticles to quench CdS@TiO 2 The method of detecting insulin has not been reported

Method used

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  • Electrochemiluminescence sensor for detecting insulin by quenching CdS hybridized TiO2 nanoribbon through gold-curcumin nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of CdS@TiO 2 chitosan solution

[0037] Disperse 0.1 g of cadmium acetate in 30 mL of ultrapure water, add 25 mg of titanium dioxide nanobelts, and sonicate for 1 h. Then, add 5 mL of 5 M Na 2 S solution, stirred for 12 h, transferred the above solution to a 100 mL reactor, reacted at 160 °C for 16 h, centrifuged, washed, and dried to obtain CdS@TiO 2 nanobelt.

[0038] The chitosan solution with a mass fraction of 0.5% was prepared by adding 0.5 g of chitosan to 100 mL of acetic acid with a volume fraction of 1%, and stirring for 2 h.

Embodiment 2

[0039] Example 2 Preparation of CdS@TiO 2 chitosan solution

[0040] Disperse 0.1 g of cadmium acetate in 30 mL of ultrapure water, add 50 mg of titanium dioxide nanobelts, and sonicate for 1 h. Then, add 5 mL of 5 M Na 2 S solution, stirred for 12 h, transferred the above solution to a 100 mL reactor, reacted at 160 °C for 16 h, centrifuged, washed, and dried to obtain CdS@TiO 2 nanobelt.

[0041] The chitosan solution with a mass fraction of 0.5% was prepared by adding 0.5 g of chitosan to 100 mL of acetic acid with a volume fraction of 1%, and stirring for 2 h.

Embodiment 3

[0042] Example 3 Preparation of Secondary Antibody-Gold-Curcumin / ZIF-8 Solution

[0043] (1) Preparation of gold-curcumin nanoparticles

[0044] First, 8 mg of curcumin was dispersed into 5 mL of dimethyl sulfoxide to form a transparent and uniform solution. Then, 2 mL of curcumin-dimethyl sulfoxide solution was added to 45 mL of ultrapure water, and stirred under reflux at 100 °C. Add 100 μL 0.1 M NaOH, when the solution is heated to 100 °C, add 1 mL 2% HAuCl 4 The solution was stirred for 1 h, cooled to room temperature, and dialyzed with a 12 kDa dialysis bag to remove unreacted impurities and small particles to obtain a gold-curcumin solution.

[0045] (2) Preparation of gold-curcumin / ZIF-8 nanoflowers

[0046] Mix 1 mL of 2-methylimidazole with 2 mL of gold-curcumin solution and stir for 10 min. Afterwards, 1 mL of 20 mM zinc acetate solution and 0.05 g of bovine serum albumin were added, and stirred for 4 h. Finally, the obtained gold-curcumin / ZIF-8 nanoflowers were...

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Abstract

The invention relates to an electrochemiluminescence sensor for detecting insulin by quenching a CdS hybridized TiO2 nanoribbon through gold-curcumin nanoparticles. According to the present invention,the potassium persulfate and the hydrogen peroxide are used as the co-reactants together, so that the electrochemiluminescence property of the CdS hybridized TiO2 nanoribbon is enhanced. In order tosensitively detect the insulin, the invention designs a sandwich type quenching type electrochemiluminescence immunosensor, and the gold-curcumin composite ZIF-8 is adopted as a quenching agent, so that the electrochemiluminescence intensity of the CdS hybrid TiO2 nanoribbon is reduced. The quenching mechanism is mainly that the curcumin consumes the hydroxyl radicals generated in an electrochemical reaction process, and the luminous intensity of the luminescent material is further reduced through energy transfer, so that the dual quenching of an electrochemiluminescence signal is realized; the insulin with different concentrations can be combined with different amounts of secondary antibody marker gold-curcumin composite ZIF-8, so that the electrochemiluminescence intensity change of thesensor is different. The linear range of insulin detection is 0.3 pg / mL-20 ng / mL, and the detection limit is 0.09 pg / mL.

Description

technical field [0001] The invention relates to a gold-curcumin nanoparticle quenching CdS hybrid TiO 2 Nanoribbon-based electrochemiluminescence sensor for insulin detection. Specifically, CdS hybrid TiO 2 Nanobelts are used as luminescent materials, and gold-curcumin nanoparticles are used as quenchers. In order to enhance the quenching effect, gold-curcumin nanoparticles are combined with ZIF-8 (gold-curcumin / ZIF-8) to prepare a detection insulin The invention discloses a quenching type electrochemiluminescence sensor, which belongs to the technical field of electrochemiluminescence detection. Background technique [0002] Insulin is a polypeptide hormone that regulates fat and carbohydrate metabolism. Clinically, the detection of insulin is very meaningful, because the content of insulin is an important indicator for evaluating endocrine function, and provides a basis for detecting diabetes, insulinoma, insulin resistance syndrome, etc. Therefore, in the present inve...

Claims

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Application Information

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IPC IPC(8): G01N27/26G01N27/30G01N27/327
CPCG01N27/26G01N27/30G01N27/3278
Inventor 李小建杜宇任祥孙晓君魏琴杨兴龙王雪莹吴丹马洪敏王欢
Owner UNIV OF JINAN
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