Nucleic acid group, kit and detection method for intrauterine microbiological detection of pregnant and lying-in women

Abstract

The invention discloses a nucleic acid group, a kit and a detection method for intrauterine microbiological detection of pregnant and lying-in women, and relates to the technical field of membrane chip detection. The nucleic acid group disclosed by the invention comprises at least one from a first primer pair, to a seventh primer pair; the sequences from the first primer pair to the seventh primerpair are shown as SEQ ID NO.3-16. The nucleic acid group can be used for simultaneously detecting pathogenic microorganisms such as group B streptococcus, gonococcus, herpes simplex virus II, human cytomegalovirus, chlamydia trachomatis, ureaplasma urealyticum, mycoplasma genitalium and the like infected in uterus of pregnant and lying-in women; when the nucleic acid group and the detection method provided by the invention are used for detection, the characteristics of high detection speed, high sensitivity, good specificity, high flux, convenience in operation and the like are achieved.

Description

technical field [0001] The invention relates to the technical field of membrane chip detection, in particular to a nucleic acid group, a kit and a detection method for detecting microorganisms in the uterus of pregnant women. Background technique [0002] According to domestic and foreign surveys, about 3%-8% of newborns are infected by maternal bacteria, viruses or protozoa every year, which is also the main cause of congenital defects or disabilities in newborns. A large number of clinical studies have pointed out that neonatal infection is closely related to intrauterine infection, and the main route of infection is the ascending reproductive tract and bloodstream infection, which can lead to pathological manifestations of various fetal organ systems or intrauterine growth restriction, fetal distress and even stillbirth , resulting in an increased incidence of neonatal disease, and is also a major risk factor for cerebral palsy in premature infants. Therefore, mastering ...

AI Technical Summary

Problems solved by technology

[0004] Microbial isolation and culture identification is the gold standard for bacteria and virus detection, but the whole process takes about 2-3 days or even longer, and the operation is cumbersome, the false negative rate is high, and the culture results are easily affected by the external environment

Serological methods are used to detect antibodies in serum, suitable for incubation period, asymptomatic patients and epidemiological investigations, including type-specific serum antibody detection, Western blotting, ELISA and other methods. This method is simple to operate, and the disadvantage is that there is no uniform standard

Qualitative PCR is used to detect target gene sequences in microorganisms. Generally, gel electrophoresis is used for analysis, which takes a short time, but has certain limitations. It cannot be detected when the yield of target fragments is low, and the sensitivity is low.

Real-time fluorescent quantitative PCR detects the amplification amount of PCR products in real time by detecting the strength of the fluorescent signal. It can quantitatively analyze the target genes in microorganisms. The detection results are accurate and repeatable, but only single or up to 3-4 multiples can be done. , cannot increase its flux

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