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Biomimetic drug-loaded nanoparticles targeting brain tumor and preparation method and application thereof

A nanoparticle, drug-loaded nanotechnology, applied in the field of medicine, can solve the problems of lack of research and reports

Active Publication Date: 2020-03-06
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Human glioma U87 cells are often used as a model to study cell proliferation and apoptosis. However, there is still a lack of research and reports on the use of biomimetic nanoparticles coated with human glioma U87 cell membranes to deliver chemotherapy drugs.

Method used

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  • Biomimetic drug-loaded nanoparticles targeting brain tumor and preparation method and application thereof
  • Biomimetic drug-loaded nanoparticles targeting brain tumor and preparation method and application thereof
  • Biomimetic drug-loaded nanoparticles targeting brain tumor and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] The specific steps for preparing biomimetic drug-loaded nanoparticles targeting brain tumors are as follows:

[0047] (1) preparing 4mL concentration is 0.4mg / mL, the PBS solution of the polyglutamic acid (PGA) that pH is 5.5;

[0048] (2) Quickly add 1mL of EDC aqueous solution with a concentration of 5.2mg / mL and 1mL of NHS aqueous solution with a concentration of 3.2mg / mL dropwise into the above solution, and stir at room temperature for 15min;

[0049] (3) Place the above solution on ice, sonicate for 5 minutes, and the ultrasonic power is 32W, and at the same time, dropwise add 8 mL of DOX solution with a concentration of 0.25 mg / mL and 0.1 mL of DOX with a concentration of 1 mg / mL and a pH of 7.4. Cys in PBS;

[0050] (4) Load the above-mentioned nanoparticle solution into a dialysis bag with a molecular weight of 3500, and dialyze with water for 24 hours to obtain purified polyglutamic acid nanoparticles loaded with doxorubicin;

[0051] (5) The glioma cell U87...

Embodiment 2

[0053] The specific steps for preparing biomimetic drug-loaded nanoparticles targeting brain tumors are as follows:

[0054] (1) preparation of 3mL concentration is 0.1mg / mL, pH is the PBS solution of polyglutamic acid (PGA) of 4;

[0055] (2) Quickly add 0.1 mL of EDC aqueous solution with a concentration of 2 mg / mL and 0.1 mL of NHS aqueous solution with a concentration of 2 mg / mL dropwise into the above solution, and stir at room temperature for 10 min;

[0056] (3) Put the above solution on ice, sonicate for 1min, and the ultrasonic power is 10W. At the same time, dropwise add 5mL of DOX solution with a concentration of 0.1mg / mL and 0.05mL of DOX with a concentration of 0.1mg / mL and a pH of 6 Cys solution in PBS;

[0057] (4) Load the above-mentioned nanoparticle solution into a dialysis bag with a molecular weight of 3500, and dialyze with water for 24 hours to obtain purified polyglutamic acid nanoparticles loaded with doxorubicin;

[0058] (5) The glioma cell U87 was ...

Embodiment 3

[0060] The specific steps for preparing biomimetic drug-loaded nanoparticles targeting brain tumors are as follows:

[0061] (1) preparing 5mL concentration is 1mg / mL, the PBS solution of the polyglutamic acid (PGA) that pH is 7;

[0062] (2) Quickly add 2 mL of EDC aqueous solution with a concentration of 10 mg / mL and 2 mL of NHS aqueous solution with a concentration of 10 mg / mL dropwise into the above solution, and stir at room temperature for 60 min;

[0063] (3) Put the above solution on ice, sonicate for 15min, and the ultrasonic power is 60W, and at the same time, add 10mL of DOX solution with a concentration of 1mg / mL and 1mL of Cys with a concentration of 2mg / mL and a pH of 8 dropwise. PBS solution;

[0064] (4) Load the above-mentioned nanoparticle solution into a dialysis bag with a molecular weight of 3500, and dialyze with water for 24 hours to obtain purified polyglutamic acid nanoparticles loaded with doxorubicin;

[0065] (5) Dissolve glioma cell U87 with neut...

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Abstract

The invention relates to the technical field of medicines. The invention relates to the field of biomimetic drugs, in particular to biomimetic drug-loaded nanoparticles targeting brain tumor and a preparation method and application thereof, and particularly relates to adriamycin-loaded polyglutamic acid biomimetic nanoparticles which target brain tumor cells and is coated by human brain glioma U87cell membranes and a preparation method and application thereof in drugs for treating brain tumor diseases. The preparation method of the biomimetic drug-loaded nanoparticles targeting brain tumor comprises the following steps: preparing adriamycin-loaded polyglutamic acid nanoparticles, and coating the surfaces of the nanoparticles with the human brain glioma U87 cell membranes to form the stable biomimetic drug-loaded nanoparticles. Therefore, higher encapsulation efficiency and better slow release performance are realized, the targeting property of the nano drug delivery system is improved, the biomimetic drug delivery nanoparticles penetrating through the blood-brain barrier can be more effectively gathered in a brain tumor area, and the effect of treating brain tumors by chemotherapeutic drugs is improved.

Description

technical field [0001] The present invention relates to the field of pharmaceutical technology, in particular to bionic drug-loaded nanoparticles targeting brain tumors and their preparation methods and applications, in particular to a human glioma U87 cell membrane-coated nanoparticle targeting brain tumor cells. The doxorubicin-loaded polyglutamic acid biomimetic nanoparticle and its preparation method and its use in the treatment of brain tumor diseases. Background technique [0002] Drug delivery system is a multidisciplinary approach to effectively deliver drugs to the target site, thereby regulating drug metabolism, pharmacodynamics, toxicity, immunogenicity, and biological recognition. The current drug delivery systems include nano-drug delivery system, long-acting sustained-release microspheres, oral sustained-release preparations and dry powder inhalation preparations, among which the most commonly used and most promising is the nano-drug delivery system. The nano-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/46A61K47/34A61K31/704A61P35/00
Inventor 蔡林涛张升平梁锐晶陈华清刘兰兰何华美
Owner SHENZHEN INST OF ADVANCED TECH
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